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القاء واکنش دفاعی پرتقال به کپک آبی (Pencillium italicum) با استفاده از اسانس گیاه ککگریز (Francoeuria undulate (L.) Lack) | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 7، دوره 34، شماره 3 - شماره پیاپی 89، مرداد و شهریور 1397، صفحه 430-442 اصل مقاله (229.31 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2018.115025.2114 | ||
| نویسندگان | ||
| محبوبهالسادات حسینی1؛ صدیقه محمدی* 2؛ علی رضا افتخاریان جهرمی2 | ||
| 1دانشجوی کارشناسی ارشد، گروه باغبانی، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران | ||
| 2استادیار، گروه بیماریشناسی، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران | ||
| چکیده | ||
| پوسیدگی کپک آبی از مهمترین بیماریها پس از برداشت میوه مرکبات است که توسط قارچ Pencillium italicum ایجاد و سالیانه منجر به از بین رفتن مقادیر زیادی از محصولات مرکبات میشود. اسانس گیاه ککگریز (Francoeuria undulate (L.) Lack) دارای خاصیت ضدباکتریایی بالایی بر میکروارگانیسمها است. بدینمنظور در دو آزمایش جداگانه، تأثیر غلظتهای مختلف اسانس (چهار سطح: صفر، 400، 800 و 1600) گیاه مذکور در القاء مقاومت میوه پرتقال در برابر کپک آبی (دو سطح: بدون بیمارگر و بیمارگر) با بررسی آنزیمهای دفاعی انجام شد. این آزمایش بهصورت فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار انجام گردید. در آزمایش دوم اثر اسانس گیاهان بر کنترل بیمارگر در چهار غلظت 0، 400، 800 و 1600 در قالب طرح کاملاً تصادفی انجام شد. نتایج تجزیه واریانس نشان داد که اثر متقابل غلظت×بیمارگر در دو بازه زمانی 48 و 96 ساعت در سه صفت آنزیم کاتالاز، پراکسیداز و فنول معنیدار گردید. همچنین نتایج تجزیه واریانس نشان داد که اثر مستقل غلظت اسانس گیاه ککگریز بر درصد بازدارندگی از رشد بیمارگر بر روی میوه معنیدار گردید. تأثیر متقابل بیمارگر× نوع گیاه نشان داد که بیشترین میزان فنول، پراکسیداز و کاتالاز مربوط به تیمارهای سالم بود اما در تیمارهای بیمار همراه ککگریز میزان بالای فنول مشاهده شد. تأثیر متقابل وجود و عدم وجود بیمارگر× غلظت نشان داد که بیشترین میزان فنول در تیمارهای سالم در غلظت صفر و ppm800 بود. پراکسیداز نیز در تیمارهای سالم بیشتر بود اما میزان کاتالاز در تیمارهای مختلف تفاوتی نداشت. تأثیر متقابل نوع گیاه- غلظت اسانس نشان داد که اختلاف آماری بین تیمارهای مختلف در میزان فنول، پراکسیداز و کاتالاز نبود. | ||
| کلیدواژهها | ||
| مقاومت؛ ککگریز (Francoeuria undulate (L.) Lack)؛ اسانس؛ کپک آبی (Pencillium italicum) | ||
| عنوان مقاله [English] | ||
| Inducing resistance reaction of orange against blue mold Pencillium italicum using the essential oil of Francoeuria undulata (L.) Lack | ||
| نویسندگان [English] | ||
| M.S. Hosseini1؛ S. Mohammadi2؛ A.R. Eftekhariyan Jahromi2 | ||
| 1M.Sc. student, Department of Horticultural Science, Shiraz Branch, Science Islamic Azad University, Shiraz, Iran | ||
| 2Department of Plant Pathology, Shiraz Branch, Islamic Azad University, Shiraz, Iran | ||
| چکیده [English] | ||
| Mold decay is one of the most important diseases after harvesting citrus fruits, produced by Pencillium italicum fungi. It annually causes high amount of losses in citrus products. The essential oil of coca bush, Francoeuria undulata (L.) Lack, has high antibacterial properties on microorganisms. Therefore, in two separate experiments, the effect of various concentrations of essential oils (four levels including 0, 400, 800 and 1600) on inducing resistance of orange's fruit was evaluated against blue mold (two levels: without pathogen and with pathogen) via studying the defense enzymes. The present study was conducted as factorial based on completely randomized design with three replications. In second experiment, the effect of essential oil on pathogen control was studied at concentrations of 0, 400, 800, and 1600 in a completely randomized design. Results of analysis of variance showed that the interaction of concentration×pathogen at 48 and 96- hour time periods was significant on three enzymes including catalase, peroxidase and phenol. Also, the results of analysis of variance showed that the effect of coca bush essential oil concentration on inhibitory of pathogen growth on fruit was significant. The interaction of pathogen× plant type showed that the highest content of phenol, peroxidase and catalase was associated with control treatments, while high phenol content was observed in pathogenic treatments with coca bush. The interaction of presence and absence of pathogen×concentration showed that the highest phenol content was obserevd in control treatments at 0 and 800 ppm concentrations. Peroxidase content was high in healthy treatments, while there was no difference among various treatments in terms of catalase content. The interaction of plant type×essential oil concentration showed that there was no significant difference among various treatments in terms of phenol, peroxidase and catalase content. | ||
| کلیدواژهها [English] | ||
| resistance, coca bush (Francoeuria undulata (L.) Lack), essential oil, Pencillium italicum | ||
| مراجع | ||
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