- همه نشریات
- نشریات علمی سازمان تحقیقات، آموزش و ترویچ کشاورزی
- مجلات ترویجی سازمان تحقیقات، آموزش و ترویج کشاورزی
- سایر نشریات سازمان تحقیقات، آموزش و ترویج کشاورزی
- نشریات علمی انجمن ها
- علوم آب و خاک
- علوم اقتصاد و ترویج کشاورزی
- علوم جنگل, مرتع و آبخیزداری
- علوم دامی و شیلات
- علوم زراعی و باغی
- علوم گیاهپزشکی
- علوم مهندسی و مکانیزاسیون کشاورزی
- نشریات با زبان انگلیسی
- نشریات با زبان فارسی
- نشریات دانشگاه آزاد اسلامی
- نشریات علمی
پیوندهای مفید
اخبار و اعلانات
آمار
| تعداد نشریات | 283 |
| تعداد نشریات سازمان | 81 |
| تعداد شماره‌ها | 2,726 |
| تعداد مقالات | 31,039 |
| تعداد مشاهده مقاله | 27,661,378 |
| تعداد دریافت فایل اصل مقاله | 16,209,371 |
Immunogenicity of Concentrated and Purified Inactivated Avian Influenza Vaccine Formulation | ||
| Archives of Razi Institute | ||
| مقاله 8، دوره 73، شماره 4، اسفند 2018، صفحه 319-324 اصل مقاله (276.73 K) | ||
| نوع مقاله: Short Communication | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2017.111120.1135 | ||
| نویسندگان | ||
| A. Nazari* 1؛ M. Samianifard2؛ I. Khalili1 | ||
| 1Department of Biochemistry and Proteomics, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran | ||
| 2Department of Viral Quality Control, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran | ||
| چکیده | ||
| Avian influenza (AI) H9N2 is a low pathogenic virus subtype belonging to Orthomyxoviridae family. Given the prevalence of this subtype as an infectious agent in poultry industry, special attention has been always directed toward the development of vaccine production against this infection. The vaccine of this infection is produced by killing the virus and using a mixture of inactivated antigen and oil phase. Egg-based viral antigens have high levels of unwanted proteins that may adversely affect the vaccine formulation. In addition, it is required to raise the antigen concentration for the production of combination vaccines, especially in low doses. This underscores the need to the improvement of the downstream purification process and concentration of antigens. The optimization of downstream processing would decrease the cost of vaccine procurement and maintenance. Regarding this, the present study was conducted to evaluate a downstream procedure for the concentration and purification of avian influenza virus (H9N2) and investigate the immunogenicity of the vaccine containing these antigens. To this end, after harvesting and clarifying virus-containing allantoic fluid, it was concentrated and purified using ultrafiltration and chromatography, respectively. The concentrated and purified samples were checked for their ovalbumin level and emulsified with oil adjuvant to access their immunogenicity. The results showed that one dose of both formulated antigens (i.e., concentrated and purified) was effective in raising the immune response in the vaccinated chicks for a long time. The applied formulation had a one-year stability in the refrigerator. Furthermore, the concentrated antigen showed a high hemagglutination activity through a year when storing in the refrigerator. Based on the findings, the optimization of downstream process of influenza (H9N2) vaccine production and use of new technologies could be considered in the large-scale preparation of a sustainable vaccine without any unwanted risk factors. | ||
| کلیدواژه‌ها | ||
| Avian H9N2 Influenza Virus؛ vaccine؛ Immunogenicity؛ Ultrafiltration | ||
| مراجع | ||
|
Alexander, D.J., 2000. A review of avian influenza in different bird species. Vet Microbiol 74, 3-13.
Alizadeh, E., Kheiri, M., Bashar, R., Tabatabaeian, M., Hosseini, S.M., Mazaheri, V., 2009. Avian Influenza (H9N2) among poultry workers in Iran. Iran J Microbiol 1, 3-6.
Butt, A.M., Siddique, S., Idrees, M., Tong, Y., 2010. Avian influenza A (H9N2): computational molecular analysis and phylogenetic characterization of viral surface proteins isolated between 1997 and 2009 from the human population. Virol J 7, 319.
Butt, K.M., Smith, G.J., Chen, H., Zhang, L.J., Leung, Y.H., Xu, K.M., et al., 2005. Human infection with an avian H9N2 influenza A virus in Hong Kong in 2003. J Clin Microbiol 43, 5760-5767.
Choi, J.G., Lee, Y.J., Kim, Y.J., Lee, E.K., Jeong, O.M., Sung, H.W., et al., 2008. An inactivated vaccine to control the current H9N2 low pathogenic avian influenza in Korea. J Vet Sci 9, 67-74.
Fusaro, A., Monne, I., Salviato, A., Valastro, V., Schivo, A., Amarin, N.M., et al., 2011. Phylogeography and evolutionary history of reassortant H9N2 viruses with potential human health implications. J Virol 85, 8413-8421.
Ghaniei, A., Allymehr, M., Moradschendi, A., 2013. Seroprevalence of avian influenza (H9N2) in broiler chickens in Northwest of Iran. Asian Pac J Trop Biomed 3, 822.
He, C., Yang, Z., Tong, K., 2011. Downstream processing of Vero cell-derived human influenza A virus (H1N1) grown in serum-free medium. J Chromatogr A 1218, 5279-5285.
Hosseini, S.M., Anvar, E., Tavasoti Kheiri, M., Mazaheri, V., Fazaei, K., Shabani, M., et al., 2013. Serological survey of avian influenza (H9N2) among different occupational groups in Tehran and Qazvin provinces in IR Iran. Jundishapur J Microbiol 6, 1-4.
Hwang, S.D., Kim, H.S., Cho, S.W., Seo, S.H., 2011. Single dose of oil-adjuvanted inactivated vaccine protects chickens from lethal infections of highly pathogenic H5N1 influenza virus. Vaccine 29, 2178-2186.
Kalbfuss, B., Wolff, M., Morenweiser, R., Reichl, U., 2007. Purification of cell culture-derived human influenza A virus by size-exclusion and anion-exchange chromatography. Biotechnol Bioeng 96, 932-944. Khalili, I., Ghadimipour, R., Sadigh Eteghad, S., Fathi Najafi, M., Ebrahimi, M.M., Godsian, N., et al., 2015. Evaluation of Immune Response Against Inactivated Avian Influenza (H9N2) Vaccine, by Using Chitosan Nanoparticles. Jundishapur J Microbiol 8, e27035. Laemmli, U.K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
Lee, C.-W., Saif, Y.M., 2009. Avian influenza virus. Comp Immunol Microbiol Infect Dis 32, 301-310.
Lee, D.H., Kwon, J.S., Lee, H.J., Lee, Y.N., Hur, W., Hong, Y.H., et al., 2011. Inactivated H9N2 avian influenza virus vaccine with gel-primed and mineral oil-boosted regimen could produce improved immune response in broiler breeders. Poult Sci 90, 1020-1022.
Lee, Y.J., Shin, J.Y., Song, M.S., Lee, Y.M., Choi, J.G., Lee, E.K., et al., 2007. Continuing evolution of H9 influenza viruses in Korean poultry. Virology 359, 313-323.
Li, C., Yu, K., Tian, G., Yu, D., Liu, L., Jing, B., et al., 2005. Evolution of H9N2 influenza viruses from domestic poultry in Mainland China. Virology 340, 70-83.
Moghaddam Pour, M., Momayez, R., Akhavizadegan, M., 2006. The efficacy of inactivated oil-emulsion H9N2 avian influenza vaccine. Iran J Vet Res 7, 85-88.
Naeem, K., Ullah, A., Manvell, R.J., Alexander, D.J., 1999. Avian influenza A subtype H9N2 in poultry in Pakistan. Vet Rec 145, 560.
OIE, A.o.D.o.t., 2015. Avian Influenza. OIE, Paris, France.
Shirvan, A.N., Samianifard, M., Ghodsian, N., 2016. Purification of avian influenza virus (H9N2) from allantoic fluidby size-exclusion chromatography. Turk J Vet Anim Sci 40, 107-111.
Vasfi Marandi, M., Bozorgmehri Fard, M.H., 2002. Isolation of H9N2 subtype of avian influenza viruses during an outbreak in chickens in Iran. Iran Biomed J 6, 13-17. | ||
|
آمار تعداد مشاهده مقاله: 5,624 تعداد دریافت فایل اصل مقاله: 732 |
||
سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب