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تأثیر غلظتهای مختلف گلیسرول و لسیتین بر ویژگیهای اسپرم گاو نر هلشتاین ایرانی در شرایط انجمـاد و یـخگشـایی پیاپی | ||
| علوم دامی | ||
| مقاله 3، دوره 31، شماره 119، شهریور 1397، صفحه 31-44 اصل مقاله (1.12 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/asj.2017.109893.1419 | ||
| نویسندگان | ||
| مریم شیبانی1؛ مهرداد معمار* 2؛ سیما نصری3 | ||
| 1دانشجوی کارشناسی ارشد،گروه فیزیولوژی جانوری دانشگاه پیام نور تهران | ||
| 2استادیار گروه فیزیولوژی علوم دامی دانشگاه یاسوج | ||
| 3دانشیار گروه فیزیولوژی جانوری دانشگاه پیام نور تهران | ||
| چکیده | ||
| بهمنظور حفاظت اسپرم و کاهش صدمات ناشی از انجماد، ترکیباتی نظیر لسیتین سویا در انجماد اسپرم گونههای مختلف مورد استفاده قرار گرفته است. انجماد پیاپی اسپرم در دامها کمتر مورد توجه بوده است که در صورت نیاز برای اسپرم نژادهای کمیاب، این فرایند میتواند ارزشمند باشد. به منظور بررسی تاثیر لسیتین و گلیسرول بر کیفت اسپرم در انجماد و یخگشایی پیاپی، نمونههای منجمد اسپرم گاو پس از یخگشایی و حذف رقیقکنندهی قبلی، با رقیق کننده جدید بر پایه بافر تریس و با غلظتهای مختلف گلیسرول (3، 5 و 7 درصد) و لسیتین سویا (1 و 2 درصد) دوباره منجمد شدند. پس از دو هفته نمونههای اسپرم یخگشایی شده و ویژگیهای اسپرم بلافاصله پس از یخگشایی و تا دو ساعت پس از نگهداری در دمای 40 درجه سانتیگراد ارزیابی شد. نتایج نشان داد که تیمارهای حاوی 5 درصد گلیسرول و یک درصد لسیتین (G5L1) و 3 درصد گلیسرول و 2 درصد لسیتین (G3L2) به ترتیب دارای بیشترین و کمترین درصد جنبایی، زندهمانی و یکپارچگی غشا اسپرم بلافاصله بعد از یخگشایی و یک ساعت بعد از نگهداری در دمای 40 درجه سانتیگراد بودند. درصد اسپرمهای جنبا و زنده در تیمار G5L1 پس از 2 ساعت نگهداری در دمای 40 درجه سانتیگراد به طور معنیداری (P<0.05) بیشتر از شاهد بود اما از لحاظ یکپارچگی غشا اسپرم با شاهد تفاوت معنیداری نداشت (P>0.05). بهطورکلی نتایج پژوهش حاضر نشان داد که استفاده از 5 درصد گلیسرول و 1 درصد لسیتین میتواند باعث بهبود ویژگیهای اسپرم در انجماد و یخگشایی پیاپی شود . | ||
| کلیدواژهها | ||
| اسپرم گاو؛ انجماد و یخ گشایی پیاپی؛ لسیتین؛ گلیسرول | ||
| عنوان مقاله [English] | ||
| The effect of different concentrations of glycerol and lecithin on the characteristics of Iranian Holstein bull sperm in repeated freezing and thawing conditions | ||
| نویسندگان [English] | ||
| maryam sheybani1؛ M. Meamar2؛ sima nasri3 | ||
| 1student | ||
| 3professor | ||
| چکیده [English] | ||
| Different compounds such as soy lecithin have been used in the sperm freezing of different species. Repeated freezing was less considered in farm animals and doing this proccess could be valuable for the rare breeds. Inorder to investigate the effect of lecithin and glycerol on sperm quality in repeated freezing and thawing, frozen bull sperm straws were thawed and the previous diluent eleminated. Then, the sperms were diluted in Tris buffer freezing extender containing different concentrations of glycerol (3, 5 and 7%) and soya lecithin (1% and 2%) and were frozen again. After two weeks, the sperms were thawed and sperm characteristics were evaluated immediately after thawing and up to two hours incubation at 40 °C. The results indicated that treatments containing 5% glycerol with one percentage lecithin (G5L1) and 3% glycerol with 2% lecithin (G3L2) had the highest and the lowest percentage of motility, viability and membrane integrity of sperm, immediately after thawing and one hour incubation at 40 °C respectively. The percentage of motile and viable sperms in G5L1 treatment was significantly higher (P <0.05) than the control group after 2 hours of incubation at 40 °C but sperm membrane integrity did not have significant difference with the control group (P>0.05). In general, the results showed that using 5% glycerol with 1% can improve the sperm characteristics in repeated freezing and thawing. | ||
| کلیدواژهها [English] | ||
| Bull sperm, Repeated freezing and thawing, lecithin, Glycerol | ||
| مراجع | ||
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Abdussamad, A., Gauly, M. and Holtz, W. (2015). Temporary storage of bovine semen cryopreserved in liquid nitrogen on dry ice and refreezing of frozenthawed semen. Cryo Letters. 36(4):278-284. Al-Badry, K.I. (2012). Effect of various thawing times and temperatures on frozen semen quality of Friesian bulls in Iraq. International Journal of Animal and Veterinary Advances. 4(60:384-388. Amirat, L., Tainturier, D., Jeanneau, L., Thorin, C., Gerard, O., Courtens, J. L. and Anton, M. (2004). Bull semen in vitro fertility after cryopreservation using egg yolk LDL: a comparison with Optidyl®, a commercial egg yolk extender. Theriogenology. 61(5):895-907. Ansari, M.S., Rakha, B.A., Akhter, S. and Ashiq, M. (2016). OPTIXcell improves the post thaw quality and fertility of buffalo bull sperm. Theriogenology. 85(3):528-532 Asadpour, R., Jafari, R. and Tayefi Nasrabadi, H. (2011). Influence of added vitamin C and vitamin E on frozen-thawed bovine sperm cryopreserved in citrate and Tris-based extenders. Veterinary Research Forum.2(1):37-44. Alvarez, M., Tamayo-canul, J., Anel, E., Boixo, J. C., Mata-Campozano, M., Martinez-Pastor, F., Anel, L. and De Paz, L. (2012). Sperm concentration at freezing affects post-thaw quality and fertility of ram semen. Theriogenology. 77:1111-1118. Barbas, J.and Mascarenhas, R. (2009). Cryopreservation of domestic animal sperm cells. Cell and Tissue Banking. 10(1):49-62. Beccaglia, M., Anastasi, P., Chigioni, S. and Luvoni, G. C. (2009). Tris-lecithin extender supplemented with antioxidant catalase for chilling of canine semen. Reproduction in domestic animals. 44(2):345-349. Bergeron, A., Crete, M. H., Brindle, Y.and Manjunath, P. (2004). Low-density lipoprotein fraction from hen's egg yolk decreases the binding of the major proteins of bovine seminal plasma to sperm and prevents lipid efflux from the sperm membrane. Biology of Reproduction. 70(3):708-717. Bowden, R.M., Ewert, M.A., Lipar, J.L. and Nelson, C. E. (2001). Concentrations of steroid hormones in layers and biopsies of chelonian egg yolks. General and Comparative Endocrinology. 121(1):95-103. Bucak, M.N., Atessahin, A. and Yuce, A. (2008). Effect of anti-oxidants and oxidative stress parameters on ram semen after the freeze–thawing process. Small Ruminant Research. 75:128-134. Del Valle, I., Gomez-Duran, A., Holt, W.V., Muino-Blanco, T. and Cebrian-Perez, J. A. (2012). Soy lecithin interferes with mitochondrial function in frozen-thawed ram spermatozoa. Journal of Andrology. 33(4):717-725. Holt, W. (2000). Basic aspects of frozen storage of semen. Animal ReproductionScience. 62(1):3-22. Jiang, Z.L., Li, Q. W., Hu, J. H., Li, W.Y., Zhao, H.W. and Zhang, S. S. (2007). Improvement of the quality of boar cryopreservation semen by supplementing with low density lipoprotein in diluents. Cryobiology. 54(3):301-304. Johnson, L., Weitze K., Fiser, P. and Maxwell, W. (2000). Storage of boar semen. Animal ReproductionScience. 62(1):143-172. Leboeuf, B., Restall, B. and Salamon, S. (2000). Production and storage of goat semen for artificial insemination. Animal reproduction science. 62:113-141. Moussa, M., Martinet, V., Trimeche, A., Tainturier, D. and Anton, M. (2002). Low density lipoproteins extracted from hen egg yolk by an easy method: cryoprotective effect on frozen–thawed bull semen. Theriogenology. 57(6):1695-1706. Najafi, A., Zhandi, M., Towhidi, A., Sharafi, M., Sharif, A. A., Motlagh, M. K. and Martinez-Pastor, F. (2013). Trehalose and glycerol have a dose-dependent synergistic effect on the post-thawing quality of ram semen cryopreserved in a soybean lecithin-based extender. Cryobiology. 66(3):275-282. Papa, F., Felicio, G., Melo, C., Alvarenga, M., De Vita, B., Avanzi, B. and Dell’Aqua, J. (2010). Effect of substituting soybean lecithin for egg yolk in an extender used for the cryopreservation of stallion semen. Animal Reproduction Science. 121(1):171-172. Papa, P.M., Maziero, R.D., Guasti, P.N., Junqueira, C.R., Freitas-Dell'Aqua, C.P., Papa, F.O., Vianna, F.P., Alvarenga, M.A., Crespilho, A.M. and Dell'Aqua, J.A. (2015). Effect of glycerol on the viability and fertility of cooled bovine semen. Theriogenology. 83:107-113. Purdy, P. (2006). A review on goat sperm cryopreservation. Small ruminant research. 63(3):215-225. Rasul, Z., Ahmed, N. and Anzar, M. (2007). Antagonist effect of DMSO on the cryoprotection ability of glycerol during cryopreservation of buffalo sperm. Theriogenology. 68:813-819. Reed, M. L., Ezeh, P. C., Hamic, A., Thompson, D. J. and Caperton, C.L. 2009. Soy lecithin replaces egg yolk for cryopreservation of human sperm without adversely affecting postthaw motility, morphology, sperm DNA integrity, or sperm binding to hyaluronate. Fertility and Sterility. 92(5):1787-1790. Salamon, S. and Maxwell, W. (2000). Storage of ram semen. AnimalReproduction Science. 62(1):77-112. Salmani, H., Towhidi, A., Zhandi, M., Bahreini, M. and Sharafi, M. (2014). In vitro assessment of soybean lecithin and egg yolk based diluents for cryopreservation of goat semen. Cryobiology. 68(2):276-280. Sharafi, M., Forouzanfar, M., Hosseini, S.M., Hajian, M., Ostadhosseini, S., Hosseini, L., Abedi, P., Nili, N., Rahmani, H.R.and Javaheri, A. R. (2009). In vitro comparison of soybean lecithin based-extender with commercially available extender for ram semen cryopreservation. InternationalJournal of Fertility and Sterility. 3(3):149-1562. Sundararaman, M., Kalatharan, J. and Jawahar, K.T.P. (2012). Computer assisted semen analysis for quantification of motion characteristics of bull sperm during cryopreservation cycle. Veterinary World. 12(5):723-726. Thun, R., Hurtado, M. and Janett, F. (2002). Comparison of Biociphos-Plus® and TRIS-egg yolk extender for cryopreservation of bull semen. Theriogenology. 57:1087-1094. Ustuner, B., Alcay, S., Nur, Z., Sagirkaya, H.and Soylu, M. K. (2014). Effect of egg yolk and soybean lecithin on tris-based extender in post-thaw ram semen quality and in vitro fertility. Kafkas Univ Vet Fak Derg. 20(3): 393-398. Van Wagtendonk-de Leeuw, A., Haring, R., Kaal-Lansbergen, L. and Den Daas, J. (2000). Fertility results using bovine semen cryopreserved with extenders based on egg yolk and soy bean extract. Theriogenology. 54(1):57-67. Vera-Munoz, O., Amirat-Briand, L., Bencharif, D., Anton, M., Desherces, S., Shmitt, E., Thorin, C. and Tainturier, D. (2011). Effect of low-density lipoproteins, spermatozoa concentration and glycerol on functional and motility parameters of bull spermatozoa during storage at 4° C. Asian Journal of Andrology. 13(2): 281-286. Yoon, S. J., Kwon, W.-S., Rahman, M.S., Lee, J.S. and Pang, M. G. (2015). A Novel Approach to Identifying Physical Markers of Cryo-Damage in Bull Spermatozoa. Plos one. doi: 10.1371/journal.pone.0126232. eCollection. Zhang, S., Hu, J., Li, Q., Jiang, Z. and Zhang, X. (2009). The cryoprotective effects of soybean lecithin on boar spermatozoa quality. African Journal of Biotechnology. 22(8):6476-6480. zubair, M., Ludhi, L. A., Ahmad, E. and Muhammad, G.( 2013). Hypo osmotic swelling test as screening for evaluation of semen of bull. Journal of Entomology and Zoology Studies. 6(1): 124-128. | ||
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