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Genetic diversity and comparison of physicochemical characteristics of three Squirting cucumber (Ecballium elaterium (L.) Rich.) populations collected in Ardabil province, Iran | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| Article 12, Volume 34, Issue 4 - Serial Number 90, September and October 2018, Pages 672-683 PDF (261.45 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.22092/ijmapr.2018.116113.2181 | ||
| Authors | ||
| A.R. Ghanbari* 1; R. Moayed Amini2; A. Estaji3; S. Fahim4; H. Ghorbani Ghozhdi5 | ||
| 1Department of Horticultural Sciences, Faculty of Agriculture and Natural Resource, University of Mohaghegh Ardabili, Ardabil, Iran | ||
| 2M.Sc. student, Department of Horticultural Sciences, Faculty of Agriculture and Natural Resource, University of Mohaghegh Ardabili, Ardabil, Iran | ||
| 3Post Doctoral Fellowships, Department of Horticultural Sciences, Faculty of Agriculture and Natural Resource, University of Mohaghegh Ardabili, Ardabil, Iran | ||
| 4Ph.D student, Department of Horticultural Sciences, Faculty of Agriculture and Natural Resource, University of Mohaghegh Ardabili, Ardabil, Iran | ||
| 5Faculty of Agriculture, Gonabad University, Gonabad, Iran | ||
| Abstract | ||
| Squirting cucumber (Ecballium elaterium (L.) Rich.) is a wild species that is native to temperate regions of Asia, North Africa, and Europe. This plant is one of the most important medicinal plants in the world. In the present study, morphological and biochemical traits of squirting cucumber ecotypes were investigated from three natural habitats in Ardabil province including Germi, Bilesuar-Anjirlooin and Parsabad regions (10 genotypes per region). The results showed that Parsabad ecotypes had large fruits and higher chlorophyll and carotenoids content. Since the genetic diversity of this plant is not been thoroughly investigated, the genetic diversity of three squirting cucumber ecotypes were examined by using ISSR markers. To assess the genetic similarity between samples a cluster analysis was performed using Jacquard. Among primers, the primer ISSR-13 showed the most polymorphism (46%). The lowest genetic similarity was found between the two genotypes of Bilesuar and Germi population. Cluster analysis of molecular data was categorized into five groups. This preliminary study demonstrated that ISSR markers were an effective method to evaluate genetic variability among squirting cucumber plant genotypes. | ||
| Keywords | ||
| Ecballium elaterium (L.) Rich; Morphological; biochemical; molecular marker; ISSR markers | ||
| References | ||
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