Mahravani, H. (2019). Evaluation of Foot and Mouth Disease virus concentration by cross flow system. , 32(1), 17-23. doi: 10.22092/vj.2018.100717.1014
H. Mahravani. "Evaluation of Foot and Mouth Disease virus concentration by cross flow system". , 32, 1, 2019, 17-23. doi: 10.22092/vj.2018.100717.1014
Mahravani, H. (2019). 'Evaluation of Foot and Mouth Disease virus concentration by cross flow system', , 32(1), pp. 17-23. doi: 10.22092/vj.2018.100717.1014
Mahravani, H. Evaluation of Foot and Mouth Disease virus concentration by cross flow system. , 2019; 32(1): 17-23. doi: 10.22092/vj.2018.100717.1014

Evaluation of Foot and Mouth Disease virus concentration by cross flow system

تحقیقات دامپزشکی و فرآورده‌های بیولوژیک
Article 2, Volume 32, Issue 1 - Serial Number 122, January 2019, Pages 17-23    PDF (584.17 K)
Document Type: Full Research Paper
DOI: 10.22092/vj.2018.100717.1014
Author
H. Mahravani*
Mehravani H., Department of FMD Vaccine Research & Production, Razi Vaccine & Serum Research Institute, AREEO, Karaj, Iran.
Abstract
Foot and Mouth Disease (FMD) vaccine is one of the inactivated vaccines and its potency depends on the antigen quality, antigen concentration per dose, and adjuvant. In this study to introduce the better antigen concentration method, both active and inactive of three serotypes O, A05, and Asia of FMD virus were used. Each of the virus type was cultivated in BHK21 cell culture and half of each viral suspension inactivated by ethylene imine. The physical method; ultra filtration (cross flow system) and the chemical method; treatment with poly ethylen glycole (PEG6000) were used for virus concentration following safety assay. The quantity of virus recovery was evaluated by virus adsorption by aluminum hydroxide gel, complement fixation, ELISA, TCID50 titration, and sucrose concentration on the collected samples. The results indicate that the concentration rate did not affect virus adoption by aluminum hydroxide gel and the quantity of virus recovery using ultra filtration was higher than PEG precipitation.
Keywords
Foot and Mouth Disease virus; Concentration; Ultra filtration; Poly ethylen glycole
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