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واکنش فلورسانس کلروفیل در ارقام گلابیِ حساس و متحمل به بیماری آتشک در شرایط کلروپلاستی فعال و غیرفعال | ||
| به زراعی نهال و بذر | ||
| مقاله 5، دوره 34، شماره 1، خرداد 1397، صفحه 73-87 اصل مقاله (260.08 K) | ||
| شناسه دیجیتال (DOI): 10.22092/sppj.2018.118102 | ||
| نویسندگان | ||
| زینب صالحی1؛ حمید عبداللهی* 2؛ سیدمهدی میری1 | ||
| 1گروه علوم باغبانی، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران | ||
| 2پژوهشکده میوه‌های معتدله و سردسیری، مؤسسه تحقیقات علوم باغبانی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران | ||
| چکیده | ||
| بیماری آتشک در اثر متقابل با درختان میزبان، خسارت قابل‌توجهی را از طریق انفجار اکسیداتیو و در نهایت نکروز بافت‌ها ایجاد می‌کند. در این تحقیق به‌منظور بررسی نقش زنجیره انتقال الکترون کلروپلاستی در این اثر متقابل، به ارزیابی واکنش فلورسانس کلروفیل در ارقام گلابی حساس و متحمل به بیماری در شرایط کلروپلاست‌های فعال و غیرفعال پرداخته شد. به‌منظور مقایسه کلروپلاست‌های فعال و غیرفعال، آزمایش‌ها در شرایط درون شیشه در حضور سوکروز (زنجیره الکترونی کلروپلاستی غیرفعال) و عدم حضور سوکروز (زنجیره الکترونی کلروپلاستی فعال) انجام شد. نتایج نشان داد پیشرفت بیماری در رقم حساس‌تر و همچنین در حضور سوکروز سریع‌تر بود. همچنین شاخص‌های فلورسانس کلروفیل حداقل (Fo) و حداکثر (Fm) در حضور سوکروز در مقایسه با شرایط عدم حضور سوکروز کاهش یافت؛ بنابراین نقش منفی حضور سوکروز در فعالیت کلروپلاست‌ها تأیید شد. مقایسه تأثیر حمله پاتوژن بر شاخص‌های فلورسانس کلروفیل حداقل و حداکثر در شرایط حضور و عدم حضور سوکروز نشانگر تأثیر حداقلی حمله پاتوژن بر شاخص حداقل فلورسانس کلروفیل در حضور سوکروز بود، در حالی که در رقم حساس و در شرایط عدم حضور سوکروز این شاخص تغییرات زیادی را نشان داد. | ||
| کلیدواژه‌ها | ||
| گلابی؛ Erwinia amylovora؛ نکروز؛ زنجیره انتقال الکترون؛ سوکروز | ||
| عنوان مقاله [English] | ||
| Chlorophyll Fluorescence Response in Susceptible and Tolerant Pear Cultivars to Fire Blight in Active and Inactive Chloroplast Conditions | ||
| نویسندگان [English] | ||
| Z. Salehi1؛ H. Abdollahi2؛ S. M. Miri31 | ||
| 1Department of Horticultural Science, Karaj Branch, Islamic Azad University, Karaj, Iran | ||
| 2Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran | ||
| چکیده [English] | ||
| Fire blight disease in interaction with host trees causes considerable damages via oxidative burst and finally necrosis on the tissues. In this research, to investigate the role of chloroplast electron transfer chain (ETC) in this interaction, the chlorophyll fluorescence response of susceptible and tolerant in vitro pear cultivars were studied in both active and inactive chloroplast conditions. For comparison of active and inactive ETC of chloroplasts, the experiments were conducted in the sucrose presence (inactive ETC) and absence (active ETC) in the growth media. The results showed faster disease progression in susceptible cultivar, as well as in sucrose presence condition. Also, the minimum (Fo) and maximum (Fm) chlorophyll fluorescence indices were reduced in sucrose presence compared to the sucrose absence condition; Therefore, the negative role of sucrose presence in the activity of chloroplasts was confirmed. Comparing the effects of pathogen attack on Fo and Fm indices in sucrose presence and absence condition showed minimum effects of pathogen attack on Fo index in the sucrose presence condition, while this index demonstrated high variation in susceptible cultivar in sucrose absence condition. | ||
| کلیدواژه‌ها [English] | ||
| Pear, Erwinia amylovora, necrosis, electron transport chain, sucrose | ||
| مراجع | ||
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Abdollahi, H., Ghahremani, Z., Erfani Nia, K., and Mehrabi, R. 2015. Role of electron transport chain of chloroplasts in oxidative burst of interaction between Erwinia amylovora and host cells. Photosynthesis Research 124: 231-242.
Abdollahi, H., Muleo, R., and Rugini, E. 2005. Evaluation of different basic salts, growth regulators and pectin effects on micropropagation of pear (Pyrus communis L.) genotypes. Seed and Plant Improvement Journal 21: 373-384 (in Persian).
Abdollahi, H., Ruzzi, M., Rugini, E., and Muleo, R. 2004. In vitro system for studying the interaction between Erwinia amylovora and genotypes of pear. Plant Cell, Tissue and Organ Culture 79: 203-212.
Anonymous. 2004. Opti-Sciences OS-30p Chlorophyll Fluorometer Operation Manual. Opti-Sciences Company, Hudson, USA. 51pp.
Azarabadi, S. R. 2014. Assessment of resistance and survey on some tolerance mechanisms to fire blight (Erwinia amylovora) in some new pear cultivars and rootstocks. M. Sc. Thesis, Varamin-Pishva Islamic Azad University, Tehran, Iran. 153pp. (in Persian).
Bhattacharjee, S. 2011. Sites of generation and physicochemical basis of formation of reactive oxygen species in plant cell. Pp. 1-30.
In: Dutta Gupta, S. (ed.). Reactive Oxygen Species and Antioxidants in Higher Plants. CRC Press, Boca Raton, FL. Erfani, J., Abdollahi, H., Ebadi, A., Fatahi Moghadam, M. R., and Arzani, K. 2013. Evaluation of fire blight resistance and the related markers in some European and Asian pear cultivars. Seed and Plant Improvement Journal 29-1: 659-672 (in Persian).
Fernandez, R. T., Perry, R. L., and Flore, J. A. 1997. Drought response of young apple trees on three rootstocks. II. Gas exchange, chlorophyll fluorescence, water relations, and leaf abscisic acid. Journal of the American Society for Horticultural Science 122: 841-848.
Fuentes, G., Talavera, C., Oropeza, C., Desjardins, Y., and Santamaria, J. M. 2005. Exogenous sucrose can decrease in vitro photosynthesis but improve field survival and growth of coconut (Cocos nucifera L.) in vitro plantlets. In Vitro Cellular & Developmental Biology-Plant 41: 69–76.
Iglesias, D. J., Tadeo, F. R., Legaz, F., Primo-Mill,o E., and Talon, M. 2001. In vivo sucrose stimulation of colour change in citrus fruit epicarps: interactions between nutritional and hormonal signals. Physiolgia Plantarum 112: 244–250.
Kautsky, H., and Hirsch, A. 1931. Neue versuche zur kohlensäureassimilation. Naturwissenschaften 19: 964-964. Khanizadeh, Sh., and Deell, J. 2005. Chlorophyll fluorescence: a new technique to screen for tolerance of strawberry flowers to spring frost. Small Fruits Review 1: 61-67.
Khodaee Chegenee, F., Abdollahi, H., Ershadee, A., and Esna Ashari, M. 2011. Determination of micro-propagation protocol for OH×F333 and OH×F69 pear clonal rootstocks. Seed and Plant Improvement Journal 27-2: 297-312 (in Persian).
Leblay, C., Chevreau, E., and Robin, L. M. 1991. Adventitious shoot regeneration from in vitro leaves of several pear cultivars (Pyrus communis L.). Plant cell, Tissue and Organ Culture 25: 99-105.
Maroofi, A., and Mostafavi, M. 1996. Evaluation of the resistance of apple, pear and quince varieties to fire blight. Acta Horticulturae 411: 395-400.
Mazarei, M., Zakeri, Z., and Hassanzadeh, N. 1994. Fire blight situation on fruit trees in West Azerbaijan and Ghazvin provinces. Iranian Journal of Plant Pathology 30: 25-32 (in Persian).
Murashige, T., and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15: 473-497.
Nourmohammadi, N., Abdollahi, H., Moeini, A., and Roohalamin, E. 2015. Effects of growth media and Fe source on micropropagation and rooting of semi-dwarf pear rootstocks, Pyrodwarf and OH×F87. Seed and Plant Improvement Journal 31-1: 265-278 (in Persian).
Oh, C. S., and Beer, S. V. 2005. Molecular genetics of Erwinia amylovora involved in the development of fire blight. FEMS Microbiology Letters 253:185–192.
Oh, C. S., Carpenter, S. C. D., Hayes, M. L., and Beer, S. V. 2010. Secretion and translocation signals and DspB/F-binding domains in the type III effector DspA/E of Erwinia amylovora. Microbiology 156:1211–1220.
Percival, G., and Henderson, A. 2003. An assessment of the freezing tolerance of urban trees using chlorophyll fluorescence. Journal of Horticultural Science and Biotechnology 78: 225-260.
Quoirin, M., and Lepoivre, P. 1977. Etude de mileux adaptes aux cultures in vitro de Prunus. Acta Horticulture 78: 437-442.
Venisse, J. S., Guller G., and Brisset M. N. 2001. Evidence for the involvement of an oxidative stress in the initiation of infection of pear by Erwinia amylovora. Plant Physiology 125: 2164-2172.
Xie, Z., and Chen, Z. 2000. Harpin-induced hypersensitive cell death is associated with altered mitochondrial functions in tobacco cells. Molecular Plant-Microbe Interaction 13: 183-190.
Yabuta, Y., Mieda, T., Rapolu, M., Nakamura, A., and Motoki, T. 2007. Light regulation of ascorbate biosynthesis is dependent on the photosynthetic electron transport chain but independent of sugars in Arabidopsis. Journal of Experimental Botany 58: 2661–2671.
Zakeri, Z., and Sharif Nabi, B. 1991. Fire blight disease in Karaj. Proceedings of the 10th Iranian Plant Pathology Congress, Kerman, Iran. Pp. 157.
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