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Molecular and micro-morphological evidences of the genus Cuscuta in Iran | ||
| Rostaniha | ||
| مقاله 3، دوره 19، شماره 2 - شماره پیاپی 56، اسفند 2018، صفحه 113-129 اصل مقاله (714.58 K) | ||
| نوع مقاله: Systematics and Biodiversity of Plants | ||
| شناسه دیجیتال (DOI): 10.22092/botany.2019.123967.1123 | ||
| نویسندگان | ||
| Elham Amini1؛ Fatemeh Nasrollahi2؛ Ali Sattarian* 3؛ Soheila Kor4؛ Sohrab Boozarpour5 | ||
| 1PhD Graduate, Department of Biology, Faculty of Sciences, Gonbad Kavous University, Gonbad, Iran | ||
| 2PhD Graduate, Department of Biology, Faculty of Sciences, University of Qom, Qom, Iran | ||
| 3Associate Prof., Department of Biology, Faculty of Sciences, Gonbad Kavous University, Gonbad, Iran | ||
| 4MSc Graduate, Department of Biology, Faculty of Sciences, Gonbad Kavous University, Gonbad, Iran | ||
| 5Assistant Prof., Department of Biology, Faculty of Sciences, Gonbad Kavous University, Gonbad, Iran | ||
| چکیده | ||
| Cuscuta is the only parasitic genus in Convolvulaceae family. This genus is globally distributed, with most species in the tropics, subtropics, and some in the temperate regions. In this study, the micro-morphological features and molecular evidencesof 12 populations from three species of Cuscuta (C. australis, C. campestris,and C. chinensis) have been considered. In total, seven quantitative and two qualitative characters of pollen were selected and measured. The most important characters include: shape, ornamentation of tectum, exine thickness and colpus length of the pollen. Based on this study, the seed shape and surface support at least for separation of C. australis from other two species. Using nuclear (nrDNA ITS) marker, we reconstructed phylogenetic relationships within three species of Cuscuta. This data set was analyzed by phylogenetic methods including Bayesian, Maximum likelihood, and Maximum parsimony. In phylogenetic analyses, all members of three species formed a well-supported clade (PP=1, ML/BS=100/100) and divided into two major clades (A and B). Clade A is composed of specimens of C. australis. Two species of C. campestris and C. chinensis are nested in clade B. Neighbor-Net diagram demonstrated separation of the studied populations. The results showed that, micro-morphological and molecular data provide reliable evidence for separation of these species. | ||
| کلیدواژه‌ها | ||
| Convolvulaceae؛ Neighbor-Net؛ nrDNA ITS؛ pollen؛ seed | ||
| عنوان مقاله [English] | ||
| شواهد مولکولی و ریزریخت‌شناختی جنس سس در ایران | ||
| نویسندگان [English] | ||
| الهام امینی1؛ فاطمه نصر الهی2؛ علی ستاریان3؛ سهیلا کر4؛ سهراب بوذرپور5 | ||
| 1دانش آموخته دکتری، گروه زیست شناسی، دانشکده علوم پایه، دانشگاه گنبد کاووس، گنبد کاووس، ایران | ||
| 2دانش آموخته دکتری، گروه زیست شناسی، دانشکده علوم پایه، دانشگاه قم، قم، ایران | ||
| 3دانشیار گروه زیست شناسی، دانشکده علوم پایه، دانشگاه گنبد کاووس، گنبد کاووس، ایران | ||
| 4دانش موخته کارشناسی ارشد، گروه زیست شناسی، دانشکده علوم پایه، دانشگاه گنبد کاووس، گنبد کاووس، ایران | ||
| 5استادیار گروه زیست شناسی، دانشکده علوم پایه، دانشگاه گنبد کاووس، گنبد کاووس، ایران | ||
| چکیده [English] | ||
| سس (Cuscuta) تنها جنس انگلی در تیره پیچکیان است. این جنس پراکنش جهانی داشته و اکثر گونه‌های آن در مناطق گرمسیری، نیمه‌گرمسیری و بعضی در مناطق معتدل پراکندگی دارند. در این مطالعه، ویژگی‌های ریزریخت‌شناختی و مولکولی 12 جمعیت از سه گونه سس (C. australis، C. campestris و C. chinensis) مورد بررسی قرار گرفت تا ارزش تشخیصی آن‌ها ارزیابی شود. در کل، هفت ویژگی کمی و دو ویژگی کیفی در گرده انتخاب شده و مورد اندازه‌گیری قرار گرفتند. مهمترین ویژگی‌ها شامل شکل، تزیینات تکتوم، ضخامت اگزین و طول شیار گرده هستند. براساس این تحقیق، شکل و سطح دانه، جدایی C. australis را از دو گونه دیگر مورد تایید قرار داد. همچنین در این تحقیق، با استفاده از نشانگر هسته‌ای (nrDNA ITS)، روابط فیلوژنتیکی در سه گونه از این آرایه بازسازی گردید. داده‌ها توسط آنالیزهای فیلوژنتیکی شامل بیزین، بیشینه درست‌‌نمایی و بیشینه صرفه‌جویی مورد بررسی قرار گرفتند. در آنالیزهای فیلوژنتیکی همه اعضای این سه گونه یک شاخه با حمایت بسیار بالا (PP=1, ML/BS=100/100) تشکیل دادند و به دو گروه اصلی تفکیک شدند (A و B). شاخه A از نمونه‌های گونه C. australis تشکیل شده و دو گونه C. campestrisو C. chinensis در شاخه B قرار گرفتند. روش شبکه-همسایه، جدایی جمعیت‌های مورد مطالعه را ثابت کرد و نتایج نشان داد که داده‌های ریزریخت‌شناختی و مولکولی، جدایی این گونه‌ها را از هم تایید می‌کنند. | ||
| کلیدواژه‌ها [English] | ||
| پیچکیان, دانه, شبکه-همسایه, فاصله‌گذار رونویسی شونده درون هسته‌ای, گرده | ||
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