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Association of polymorphisms of FASN gene with productive and reproductive traits in Iranian Holstein cows of Kerman province | ||
| علوم دامی | ||
| Article 20, Volume 31, Issue 121, March 2019, Pages 253-262 PDF (1.65 M) | ||
| Document Type: Research Paper | ||
| DOI: 10.22092/asj.2018.120376.1622 | ||
| Authors | ||
| Hagar Rezakhani nejad1; Gholam Reza Dashab* 2; Mehdi Vafa Valleh3 | ||
| 1MS.c of Animal Breeding, Department of Animal Science, University of Zabol | ||
| 2Assistant Professor of University of Zabol | ||
| 3Assistant Professor, Animal Breeding and Genetics, University of Zabol | ||
| Abstract | ||
| Fatty acid synthase (FASN), a multifunctional protein that carries out the synthesis of fatty acids, is coded by FASN gene which is known as major candied gene and plays a central role in de novo lipogenesis in mammals. This study was conducted to detect single nucleotide polymorphisms (SNPs) of the FASN gene and explore their relationships with three economically important traits including milk production (M), calving interval (CI) and conception rate (CR) in Holstein cows of Kerman province. The whole blood samples corresponding to the 38 Holstein cows with high and low estimate breeding values were collected. Genomic DNA was extracted from blood samples of the cows using a genomic DNA kit according to the manufacturer’s instructions. The genetic material was used for polymerase chain reaction amplification of selected gene fragments (750bp length from exon 37 to 39). All of PCR products were subsequently used directly for sequencing. Analysis of the target sequence followed by multiple regression of average allele substitution effects on M, CI, and CR traits revealed 58 SNP markers, among which 8 SNPs were totally associated with productive and reproductive traits. (M), (CI) and (CR) were affected by three (g.16593A>G, g.16670C>A and g.16776C>T), three (g.16524G,C,T>A, g.16830G,C,T>A, g.16833A,T>G) and two (g.16594A,C>G and g.16811A,T>G) SNPs respectively (P<0.05). Association between polymorphism in FASN gene and traits could be used as molecular markers for determination of animal's genetic potential as well as breeding strategies in Holstein population. | ||
| Keywords | ||
| FASN gene; Sequencing; Polymorphism; Holstein cattle | ||
| References | ||
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