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Effect of gamma irradiation on microbial decontamination and crude nutrient content and mineral nutrient composition of laboratory animal diets | ||
| Archives of Razi Institute | ||
| مقالات آماده انتشار، پذیرفته شده ، انتشار آنلاین از تاریخ 26 اسفند 1397 | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2017.116153.1160 | ||
| نویسندگان | ||
Gholamreza Shahhosseini1؛ Abbas Karimi  2؛ Saeid Amanpour3؛ Mohammad Ali Mansouri4
| ||
| 1Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute, Karaj, Iran. | ||
| 2Department of Molecular Medicine, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran. | ||
| 3Cancer Biology Research Center, Tehran University of Medical Sciences, Tehran, Iran | ||
| 4Department of Research, Breeding and Production of Laboratory Animals, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran. | ||
| چکیده | ||
| Abstract Laboratory animals’ models are an important part of a test design. Certain conditions such as microbial contamination in diets of these models could affect the results of experiments. One of the most important routes predisposing contamination is generated through feeding of laboratory animals. This study aimed to show the effect of gamma irradiation in reducing bacteria concentrations and the crude nutrient content and the concentrations of some minerals and trace elements in laboratory animal diets. Large-sized pellets with 10-15 mm diameter (commonly used for rats and hamsters) and small-sized pellets with 3-5 mm diameter (used for rabbit and guinea pig) along with skimmed milk powder (SMP) as a food additive were exposed to gamma irradiation with different doses ranging from 3 to 30 kGy. The total microbial contamination and any possible changes in some mineral nutrient composition and the crude nutrient content were determined pre- and post-irradiation. Our data revealed that 25 kGy in pelleted diets and 18 kGy in SKM had superior effects in the reduction of bacterial contamination with some little change in crude nutrient content and minerals and trace elements in nutrient requirements of laboratory animals. According to the results, gamma irradiation had minimal effects on crude nutrient content and the concentrations of some minerals and trace elements of laboratory animal diets and it also eliminated bacterial and fungal contamination load. By using gamma irradiation, this method could yield a favorable outcome in controlling microbial contamination of animal diets. Keyword: Gamma Irradiation, laboratory animal, Diet, Bacteria, Pellet Abbreviations: kGy; KiloGray, SPF; specific pathogen-free | ||
| کلیدواژه‌ها | ||
| Food Irradiation؛ laboratory animal؛ Diet؛ Bacteria | ||
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آمار تعداد مشاهده مقاله: 2 |
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