A case of identity confirmation of Brucella abortus S99 by phage typing and PCR methods

alamian, saeed; Dadar, Maryam; Behrozikhah, Ali Mohammad; Soleimani, Sina; Etemadi, Afshar

doi:10.22092/ari.2019.123507.1255

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	A case of identity confirmation of Brucella abortus S99 by phage typing and PCR methods
Archives of Razi Institute
مقالات آماده انتشار، پذیرفته شده ، انتشار آنلاین از تاریخ 26 اسفند 1397
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2019.123507.1255
نویسندگان
saeed alamian ¹؛ Maryam Dadar ¹؛ Ali Mohammad Behrozikhah²؛ Sina Soleimani ³؛ Afshar Etemadi⁴
¹Assistant professor of Brucellosis Department, Razi Vaccine and Serum Research Institute (RVSRI); Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
²Brucellosis Department, Razi Vaccine and Serum Research Institute (RVSRI); Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
³Razi Vaccine and Serum Research Institute (RVSRI); Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
⁴Brucellosis Department, Razi Vaccine and Serum Research Institute (RVSRI); Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
چکیده
Brucellosis is a zoonotic infection that is associated with fever in humans and abortion in animals. The agent of this disease is facultative intracellular gram-negative coccobacillus called Brucella. There are six classic species, including B.abortus, B.melitensis, B. suis, B. canis, B. neotomae and B. ovis. In recent years, four new species have been reported, including Brucella ceti, B. microti, B. pinnipedialis and B. inopinata. Human disease causes hygienic and economic losses, including the inactivity of work forces in the community and the high cost of treatment. The disease also causes catastrophic losses in the livestock industry. There is no effective vaccine against human brucellosis. Hence, attempts to prevent human infection with this disease are focused on preventative measures, so control of infection in livestock can lead to a reduction in its incidence in humans. The common methods for diagnosis of this disease are serologic methods including Rose Bengal, Wright -2 ME agglutination and ring test. B. abortus strain S99 is used to produce these diagnostic antigens. The production of these antigens requires the presence of a well characterized seed with full identity. The aim of this work was the confirmation of identity of B. abortus S99 by phage typing, AMOS and Multiplex PCR techniques. Therefore, it is essential to carry out the identification of the strains used as seed for the production of the diagnostic brucellosis antigens. In this project, B. abortus strain 99 was supplied by the bacterial collection of Razi Vaccine and Serum Research Institute, Brucellosis department. Then, the main aim of the present study was the confirmation of the seed identity by doing the tests through the standard phage typing method, AMOS PCR and multiplex PCR (Brucladder) methods, were performed. Results were in support of the identity of the studied strain and the molecular methods could be also used as the sensitive approaches for validation of antigenic seed.
کلیدواژه‌ها
molecular test؛ antigenic seed؛ Brucella abortus S99؛ Validation


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A case of identity confirmation of Brucella abortus S99 by phage typing and PCR methods