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Jafari, Hedieh, Nemati, Mohammad, Haddad Molayan, Pedram, Khaleghi Rostamkolaie, Lida, Hamidi Nejat, Hossein. (1398). The effect of Mesobuthus epeus venom on the viability of hydatid cyst protoscoleces in vitro. سامانه مدیریت نشریات علمی, (), -. doi: 10.22092/ari.2018.121416.1213
Hedieh Jafari; Mohammad Nemati; Pedram Haddad Molayan; Lida Khaleghi Rostamkolaie; Hossein Hamidi Nejat. "The effect of Mesobuthus epeus venom on the viability of hydatid cyst protoscoleces in vitro". سامانه مدیریت نشریات علمی, , , 1398, -. doi: 10.22092/ari.2018.121416.1213
Jafari, Hedieh, Nemati, Mohammad, Haddad Molayan, Pedram, Khaleghi Rostamkolaie, Lida, Hamidi Nejat, Hossein. (1398). 'The effect of Mesobuthus epeus venom on the viability of hydatid cyst protoscoleces in vitro', سامانه مدیریت نشریات علمی, (), pp. -. doi: 10.22092/ari.2018.121416.1213
Jafari, Hedieh, Nemati, Mohammad, Haddad Molayan, Pedram, Khaleghi Rostamkolaie, Lida, Hamidi Nejat, Hossein. The effect of Mesobuthus epeus venom on the viability of hydatid cyst protoscoleces in vitro. سامانه مدیریت نشریات علمی, 1398; (): -. doi: 10.22092/ari.2018.121416.1213

The effect of Mesobuthus epeus venom on the viability of hydatid cyst protoscoleces in vitro

Archives of Razi Institute
مقالات آماده انتشار، پذیرفته شده ، انتشار آنلاین از تاریخ 15 اردیبهشت 1398  XML
نوع مقاله: Short Communication
شناسه دیجیتال (DOI): 10.22092/ari.2018.121416.1213
نویسندگان
Hedieh Jafari email orcid 1؛ Mohammad Nemati1؛ Pedram Haddad Molayan2؛ Lida Khaleghi Rostamkolaie2؛ Hossein Hamidi Nejat2
1Department of Venomous Animals and Toxins. Razi Vaccine & Serum Research Institute, Ahvaz, Agriculture Research, Education and Extension Organization (AREEO).
2Department of pathobiology, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
چکیده
Hydatidosis is an important zoonosis caused by parasitic tapeworm, Echinococcus granulosus. This infection is distributed worldwide and affects the health as well as economic loss in both humans and animals. In most of the cases disease needs chemotherapy with or without surgery. Conventional drugs have difficulties such as complications, side effects and also progressive resistance. According to importance of biological productions as alternative medicine, literature affirms that whole venom and many peptide ingredients of the scorpion venom have many different medical benefits including antimicrobial properties mainly by blocking of ion-gated channels mechanism. In this study the venom peptides of Mesobuthus eupeus scorpion were purified using gel filtration chromatography and subsequently with ion exchange chromatography and then molecular weights of the proteins were determined by SDS-PAGE procedure. After collecting the hydatid cysts fluids from the liver of infected sheep, protoscolices were derived, washed and encountered to whole venom and also 8 different fractions of toxin 30, 60, 120 and 240 minutes after exposure. After that, the viability of protoscolices was determined by eosin staining. Results revealed that a venom fraction under 10 kDa killed all protoscolices during 30 mins. Also results showed that scolocidal activity of fractions increases according to the time of exposure. We concluded that M. epeus venom peptides under its LD50 can properly and quickly destroy protoscolices of hydatid cysts at the level of applied concentrations and such these components are good alternatives to treat these organisms especially when a surgeon aim to prepare the patient before surgery
کلیدواژه‌ها
Hydatid cyst؛ Protoscolices؛ Mesobuthus eupeus؛ Venom؛ Treatment
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تعداد مشاهده مقاله: 5


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