Najarnezhad, V., Ownagh, A., Mirzakhani, N., Raeesi, H. (2019). Assessment on therapeutic effects of water extract of garlic on experimental cutaneous candidiasis in goats. , 32(3), 74-81. doi: 10.22092/vj.2019.123297.1496
Vahid Najarnezhad; Abdolghaffar Ownagh; Navideh Mirzakhani; HamidReza Raeesi. "Assessment on therapeutic effects of water extract of garlic on experimental cutaneous candidiasis in goats". , 32, 3, 2019, 74-81. doi: 10.22092/vj.2019.123297.1496
Najarnezhad, V., Ownagh, A., Mirzakhani, N., Raeesi, H. (2019). 'Assessment on therapeutic effects of water extract of garlic on experimental cutaneous candidiasis in goats', , 32(3), pp. 74-81. doi: 10.22092/vj.2019.123297.1496
Najarnezhad, V., Ownagh, A., Mirzakhani, N., Raeesi, H. Assessment on therapeutic effects of water extract of garlic on experimental cutaneous candidiasis in goats. , 2019; 32(3): 74-81. doi: 10.22092/vj.2019.123297.1496

Assessment on therapeutic effects of water extract of garlic on experimental cutaneous candidiasis in goats

تحقیقات دامپزشکی و فرآورده‌های بیولوژیک
Article 8, Volume 32, Issue 3, September 2019, Pages 74-81    PDF (2.62 M)
Document Type: Full Research Paper
DOI: 10.22092/vj.2019.123297.1496
Authors
Vahid Najarnezhad* 1; Abdolghaffar Ownagh2; Navideh Mirzakhani3; HamidReza Raeesi1
1Department of Internal Medicine, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
2Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
3Department of Clinical Pothology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Abstract
Many therapeutic effects have been known for garlic, so far. It has been used for health and treatment of various diseases in Iranian traditional medicine. This study was conducted to evaluate the therapeutic effects of water extract of garlic on experimental cutaneous candidiasis in goats. In this study four 8-month-old goats were used. After weakening the immune system, each animal were inoculated in four points with 0.1 ml of Candida albicans suspension (2.5×106 cells/ml) intracutaneously (A: Right side of the chest for treatment with garlic extract, B: Left side of the chest for nystatin treatment, C: Right flank for treatment with distilled water, and D: Left flank without treatment). Histopathological finding were identified as, cutaneous swelling, vesicles formation, redness and scaling of the skin, four days after Candida albicans inoculation. Clinical signs of cutaneous candidiasis were completely recovered, after the administration of garlic extract for 5 days and nystatin for 7 days. Cutaneous injuries created in non-treated and distilled water-treated groups opened to the skin surface and creamy secretions released. The results of this study showed that water extract of garlic can repair cutaneous candidiasis lesions in a shorter time period compared to nystatin in goat.
Keywords
Candida albicans; Cutaneous candidiasis; Water extract of Garlic; Goat
References

1. Aala, F., U. K. Yusuf, F. Jamal, and S. Rezaie. 2012. Antimicrobial effects of allicin and ketoconazole on trichophyton rubrum under in vitro condition. Brazilian Journal of Microbiology 43: 786-792.
2. Appel, E., A. Vallon-Eberhard, A. Rabinkov, O. Brenner, I. Shin, K. Sasson, Y. Shadkchan, N. Osherov, S. Jung, and D. Mirelman. 2010. Therapy of murine pulmonary aspergillosis with antibody-alliinase conjugates and alliin. Antimicrob Agents Chemother 54: 898-906.
3. Fani, M.M., and A. M. Araghizadeh. 2009. Anti fungal activities of fresh garlic extract on Candida albicans. Hormozgan Medical Journal 13: 143-148.
4. Ghazanfari, T., Z. Hassan, and A. Khamesipour. 2006. Enhancement of peritoneal macrophage phagocytic activity against Leishmania major by garlic (Allium Sativum) treatment. Journal of Ethnopharmacology 103: 333-337.
5. Gruenwald, J., T. Brendler, and C. Jaenicke. 2008. PDR for Herbal Medicines. Montvale: Medical Economics Company 327-328.
6. Jubb, K.V.F., P. C. Kennedy, and N. Palmer. 2016. Pathology of domestic animals, 6 ed. United States of America: Elsevier saunders
7. Khodavandi, A., F. Alizadeh, F. Aala, Z. Sekawi, and P. P. Chong. 2010. In vitro investigation of antifungal activity of allicin alone and in combination with azoles against Candida species. Mycopathologia 169: 287-295.
8. Khosravi, A., H. Shokri, and R. Yahyarayat. 2005. Veterinary mycology, 1 ed. Tehran, Iran: Jahaddaneshgahi Press
9. Khosravi, A.R., H. Mardjanmehr, H. Shokri, R. Naghshineh, M. Rostamibashman, and A. Naseri. 2009. Mycological and histopathological findings of experimental disseminated candidiasis in dogs. Iranian Journal of Veterinary Research 10: 228-234.
10. Lanzotti, V., E. Barile, V. Antignani, G. Bonanomi, and F. Scala. 2012. Antifungal saponins from bulbs of garlic, Allium sativum L. var. Voghiera. Phytochemistry 78: 126-134.
11. Ledezma, E., and R. Apitz-Castro. 2006. Ajoene the main active compound of garlic (Allium sativum): a new antifungal agent. Revista Iberoamericana Micologia 23: 75-80.
12. Lemar, K.M., M. A. Aon, S. Cortassa, B. O’Rourke, C. T. Müller, and D. Lloyd. 2007. Diallyl disulphide depletes glutathione in Candida albicans: oxidative stress-mediated cell death studied by two-photon microscopy. Yeast 24: 695-706.
13. MacCallum, D. 2011. Hosting Infection: ExperimentalModels to Assay Candida Virulence. International Journal of Microbiology 43: 1-12.
14. Mohammadzadeh, S., M. Shariatpanahi, M. Hamedi, R. Ahmadkhaniha, N. Samadi, and S. Ostad. 2007. Chemical composition, oral toxicity and antimicrobial activity of Iranian Propolis. Food Chemistry 103: 1097-1103.
15. Ogita, A., K. Fujita, M. Taniguchi, and T. Tanaka. 2006. Enhancement of the fungicidal activity of amphotericin B by allicin, an allyl-sulfur compound from garlic, against the yeast Saccharomyces cerevisiae as a model system. Planta Medica 72: 1247-1250.
16. Ogita, A., Y. Nagao, K. Fujita, and T. Tanaka. 2007. Amplification of vacuole-targeting fungicidal activity of antibacterial antibiotic polymyxin B by allicin, an allyl sulfur compound from garlic. The Journal of Antibiotics 169: 287-295.
17. Pedraz, J., Y. Delgado-Jime, S. Perez-Gala, S. Nam-Che, J. Fernandez-Herrena, and A. Garcia-Diez. 2009. Cutaneous expression of systemic candidiasis. Clinical and Experimental Dermatology 34: 106-110.
18. Pupim, A.C.E., T. G. Campois, E. J. Almeida Araújo, T. I. Estivalet Svidizinski, and I. Felipe. 2017. Infection and tissue repair of experimental cutaneous candidiasis in diabetic mice. Journal of Medical Microbiology 66: 808-815.
19. Rahman, D., M. Mistry, S. Thavaraj, and S. J. Challacombe. 2007. Murine model of concurrent oral and vaginal Candida albicans colonization to study epithelial host pathogen interactions. Microbes and Infection 9: 615-622.
20. Rhew, Z., and Y. Han. 2016. Synergic effect of combination of glycyrol and fluconazole against experimental cutaneous candidiasis due to Candida albicans. Archives of Pharmacal Research 39: 1482-1489.
21. Segal, E., and M. Frenkel. 2018. Experimental in vivo models of candidiasis. Journal of Fungi 21: 2-10.
22. Shams-Ghahfarokhi, M., M. R. Shokoohamiri, N. Amirrajab, B. Moghadasi, A. Ghajari, F. Zeini, G. Sadeghi, and M. Rezzaghi-Abyaneh. 2006. In vitro antifungal activities of Allium cepa, Allium sativum and ketoconazole against some pathogenic yeasts and dermatophytes. Fitoterapia 77: 321-323.
23. Sullivan, D., G. Moran, E. Pinjon, A. Al-Mosaid, C. Stokes, C. Vaughan, and D. C. Coleman. 2004. Comparison of the epidemiology, drug resistance mechanisms, and virulence of Candida dubliniensis and Candida albicans. FEMS Yeast Research 4: 369-376.
24. Yoshida, S., Sh. Kasuga, N. Hayashi, T. Ushiroguchi, H. Matsuura, and S. Nakagawa. 1987. Antifungal activity of ajoene derived from garlic. Applied and Environmental Microbiology 53: 615-617.
25. Yousuf, S., A. Ahmad, A. Khan, N. Manzoor, and L. A. Khan. 2010. Effect of diallyldisulphide on an antioxidant enzyme system in Candida species. Canadian Journal of Microbiology 56: 816-821.

Statistics
Article View: 740
PDF Download: 564


counter
Journal management system. designed by sinaweb