| تعداد نشریات | 284 |
| تعداد نشریات سازمان | 82 |
| تعداد شماره‌ها | 3,037 |
| تعداد مقالات | 33,984 |
| تعداد مشاهده مقاله | 45,795,011 |
| تعداد دریافت فایل اصل مقاله | 77,195,950 |
بهبود کارآیی بذرهای زوال یافته کدوی پوست کاغذی با استفاده از پیش‌تیمار نیترو پروساید سدیم تحت تنش خشکی | ||
| علوم و فناوری بذر ایران | ||
| مقاله 6، دوره 8، شماره 1 - شماره پیاپی 15، تیر 1398، صفحه 67-81 اصل مقاله (1.1 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22034/ijsst.2019.114505.1108 | ||
| نویسندگان | ||
| حسین رضا روحی* 1؛ علی مرادی2؛ مریم ثمن3؛ یاسین محمدی4؛ علی رضا شاهبداغلو5 | ||
| 1دانشگاه بوعلی سینا | ||
| 2استادیار گروه زراعت و اصلاح نباتات دانشگاه یاسوج | ||
| 3دانشگاه پیام نور | ||
| 4دانشجوی کارشناسی ارشد سابق علوم و تکنولوژی بذر | ||
| 5کارشناس پژوهشی | ||
| چکیده | ||
| به‌منظور بررسی اثر نیترو پروساید سدیم به عنوان ترکیب آزاد کتتده اکسید نیتروژن در بهبود کارآیی بذرهای زوال یافته کدوی پوست کاغذی تحت تنش خشکی، آزمایشی به‌صورت فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار در آزمایشگاه تکنولوژی بذر دانشکده کشاورزی دانشگاه بوعلی سینا همدان انجام شد. پیش‌تیمار غلظت‌های مختلف نیتروپروساید سدیم شامل صفر (پیش‌تیمار شده با آب)، 25، 50 و 75 میکرومولار در سطوح خشکی صفر، 2/0-، 4/0- و 6/0- مگاپاسکال مورد ارزیابی قرار گرفت. شاخص‌های درصد جوانه‌زنی، سرعت جوانه‌زنی، بنیه بذر، طول گیاهچه، هیدرات‌های کربن محلول، پروتئین‌های محلول، هدایت الکترولیتی غشاء، مالون دی‌آلدهید، فعالیت آنزیم های کاتالاز، سوپراکسید دیسموتاز و آسکوربات پراکسیداز بررسی شد. نتایج نشان داد پیش‌تیمار بذرها با غلظت‌های مختلف نیترو پروساید سدیم، از کاهش معنی‌دار شاخص‌های جوانه‌زنی بذرهای زوال یافته کدوی پوست کاغذی تحت تنش خشکی جلوگیری نمود. به‌طوری‌که در پتانسیل 6/0- مگاپاسکال، پیش‌تیمار بذرها با 75 میکرومولار نیترو پروساید سدیم سبب افزایش سرعت جوانه‌زنی، شاخص بنیه بذر، قندها و پروتئین‌های محلول به-ترتیب به‌میزان 25، 1/214، 8/90، 1/117 درصد و برای فعالیت آنزیم‌های کاتالاز، سوپراکسید دیسموتاز و آسکوربات پراکسیداز به‌ترتیب حدود 34، 5/14و 4/41 درصد نسبت به شاهد گردید. بنابراین پیش‌تیمار نیتروپروساید سدیم به‌ویژه با غلظت 75 میکرومولار به دلیل کاهش تنش اکسیداتیو ناشی از زوال و خشکی برای کدوی پوست کاغذی قابل توصیه است. | ||
| کلیدواژه‌ها | ||
| پیش‌تیمار؛ جوانه‌زنی؛ زوال بذر؛ کدوی پوست کاغذی؛ نیترو پروساید سدیم | ||
| عنوان مقاله [English] | ||
| Seed priming with SNP improves the performance of aged pumpkin (Cucurbita pepo L.) seeds under drought stress | ||
| نویسندگان [English] | ||
| Hossein Reza Rouhi1؛ َAli Moradi2؛ Maryam Saman3؛ Yasin Mohammadi4؛ Alireza Shahbodaghlo5 | ||
| 1گروه زراعت و اصلاح نباتات | ||
| 2Associate Professor, Department of Agronomy and Plant breeding, Faculty of Agriculture, University of Yasouj, Iran | ||
| 3گروه علوم کشاورزی، تهران | ||
| 4دانشکده علوم و مهندسی کشاورزی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، | ||
| 5گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه بوعلی سینا | ||
| چکیده [English] | ||
| In order to investigate the effect of sodium nitroprusside (NO donor) on improvement of aged pumpkin seeds under drought stress, a factorial experiment was carried out based on completely randomized design with three replications in Bu-Ali Sina University, Hamedan, Iran. Seed priming with different concentrations of sodium nitroprusside including 0 (hydropriming), 25, 50 and 75 µM in drought stress levels (0, -0.2, -0.46 and -0.6 MPa) was performed. Germination percentage, germination rate, seed vigor, seedling length, soluble carbohydrates, soluble proteins, memberane electrical conductivity, malondialdehyde content and antioxidant enzymes activity (catalase, ascorbate peroxidase and superoxide dismutase) were evaluated. The results showed that seed priming with different concentrations of sodium nitroprusside mitigated significant decrease of germination parameters of aged seeds under drought stress. At -0.6 MPa, seed priming with 75 µM of sodium nitroprusside increased germination rate, vigor index , soluble carbohydrates and proteins 25, 214.1, 90.8, 117.1%, as well as the activity of catalase, superoxide dismutase and ascorbate peroxidase increased by 34, 14.5, 41.4% in comparison to control, respectively. Therefore, seed priming with sodium nitroprusside specialy with 75 µM is suggested for aged pumpkin seeds by decreasing oxidative stress due to drought stress. | ||
| کلیدواژه‌ها [English] | ||
| germination, pumpkin, seed deterioration, seed priming, sodium nitroprusside | ||
| مراجع | ||
|
Abbasi, A.R., R. Sarvastani, B. Mohammadi, and A. Bagheri. 2014. Drought stress induced change at physiological and biochemical levels in some common vetch (Vicia sativa L.) genotypes. J. Agric. Sci. Technol. 505–516. Arc, E., J. Sechet, F. Corbineau, L. Rajjou, and A. Marionpoll. 2013. ABA crosstalk with ethylene and nitric oxide in seed dormancy and germination. Front. Plant Sci. 4: 1–19. Asadi-Sanam, S., M. Zavareh, and A. Hashempour. 2015. Protective effect of exogenous nitric oxide on alleviation of oxidative damage induced by high salinity in rice (Oryza sativa L.) seedlings. Iran Agric. Res. 34(1): 63–70. Besson-Bard, A., C. Courtois, A. Gauthier, J. Dahan, G. Dobrowolska, S. Jeandroz, and D. Wendehenne. 2008. Nitric oxide in plants: production and cross-talk with Ca 2+ signaling. Molecular Plant. 1(2): 218-228. Bradford, M.M. 1976. A dye binding assay for protein. Analyt Biochem.72: 248-254. Cakmak, I., and W. Horst. 1991. Effect of aluminium on lipid peroxidation, superoxide dismutase, catalase and peroxidase activities in root tip of soybean (Glycine max). Plant Physiol. 83: 463–468. Cavalcanti, F.R., J.T.A. Oliveira, A.S. Martins-Miranda, R.A. Viégas, and J.A.G. Silveira. 2004. Superoxide dismutase, catalase and peroxidase activities do not confer protection against oxidative damage in salt-stressed cowpeas leaves. New Phytol. 163: 563–571. Delouche, J.C., and C.C. Baskin. 1973. Accelerated ageing technique for predicting relative storability of seed lots. Seed Sci. Technol. 1: 427-452. Dong, L., Z. Hao, Z. Li, and Q. Wang. 2014.Enhancement of welsh onion (Allium fistulosum L.) seed vigor by KNO3 priming. J. Agric. Sci. Technol. 16: 1345–1353. Ellis, R.A., and E.H. Roberts. 1981.The quantification of ageing and survival in orthodox seeds. Seed Sci. Technol. 9: 373–409. Fu, Y.B., Z. Ahmed, and A. Diederichsen. 2015. Towards a better monitoring of seed ageing under ex situ seed conservation. Conserv. Physiol. 3: 1–16. Garcı́a-Mata, C., and L. Lamattina. 2001. Nitric oxide induces stomatal closure and enhances the adaptive plant responses against drought stress. Plant Physiol. 126(3): 1196-1204. Giannopolitis, C., and S. Ries. 1977. Superoxid desmutase. I: Occurence in higher plant. Plant Physiol. 59: 309–314. Hampton, J.G., and D.M. TeKrony. 1995. Handbook of Vigour Test Methods. The international Seed Testing Association, Zurikh. Hamzei, J., and M. Babaei, 2015.Effect of irrigation and nitrogen fertilizing on phenology, grain yield and oil of pumpkin (Cucurbita pepo L.) in Hamadan region. J. Agric. Sci. Sustain. Prod. 25(2.1): 1-13. Hayat, S., S. Yadav, M.N. Alyemeni, and A. Ahmad. 2014. Effect of sodium nitroprusside on the germination and antioxidant activities of tomato (Lycopersicon esculentum Mill.). Bulg. J. Agric. Sci. 20 (1): 140–144. He, J., Y. Ren, X. Chen, and H. Chen. 2014.Protective roles of nitric oxide on seed germination and seedling growth of rice (Oryza sativa L.) under cadmium stress. Ecotoxicol. Environ. Safety. 108: 114-119. Hou, L., W. Liu, Z. Li, C. Huang, X.L. Fang, Q. Wang, and X. Liu. 2014. Identification and Expression Analysis of Genes Responsive to Drought Stress in Peanut. Russ. J. Plant Physiol. 61(6): 842–852. Irigoyen, J.J., D.W. Emerich, and M. Sanchez-Diaz. 1992. Water stress induced changes in concentrations of proline and total soluble sugars in nodulated alfalfa (Medicago sativa) plants. Physiol. Plant. 84: 55-60. ISTA. 2007. International Rules for Seed Testing. Seed Sci. Technol. 13: 299–520. Jisha, K.C., K. Vijayakumari, and J.T. Puthur. 2013. Seed priming for abiotic stress tolerance: an overview. Acta Physiol. Plant. 35: 1381–1396. Jyoti, and C.P. Malik. 2013. Seed deterioration: a review. Int. J. Life Sci. Biotechnol. Pharma Res. 2(3): 374–385. Job, C., L. Rajjou Lovigny, M. Belghazi and D. Job. 2005. Patterns of protein oxidation in Arabidopsis seeds and during germination. Plant Physiol. 138: 790–802. Kapoor, R., A. Arya, M.A. Siddiqui, A. Amir, and H. Kumar. 2010. Seed Deterioration in Chickpea (Cicer arietinum L.) under Accelerated Ageing. Asian J. Plant Sci. 9(3): 158-162. Kibinza, S., J. Bazin, C. Bailly, J.M. Farrant, F. Corbineau, and H. El-Marrouf Bouteau. 2011. Catalase is a key enzyme in seed recovery from ageing during priming. Plant Sci. 181: 309-315. Li, X., H. Jiang, F. Liu, J. Cai, T. Dai, W. Cao, and D. Jinag. 2013. Induction of chilling tolerance in wheat during germination by pre-soaking seed with nitric oxide and gibberellin. Plant Growth Regul. 71: 31–40. Liu, X., L. Wang, L. Liu, Y. Guo, and H. Ren. 2013. Alleviating effect of exogenous nitric oxide in cucumber seedling against chilling stress. Afr. J. Biotechnol. 10(21): 4380-4386. Liu, H.Y., X. Yu, D. Cui, M. Sun, W. Sun, Z. Tang, S. Kwak, and W. Su. 2007. The role of water channel proteins and nitric oxide signaling in rice seed germination. Cell Res. 17: 638-649. Manai, J., H. Gouia, and F. J. Corpas. 2014. Redox and nitric oxide homeostasis are affected in tomato (Solanum lycopersicum L.) roots under salinity-induced oxidative stress. J. Plant Physiol. 171(12): 1028-1035. Michel, B.E., and M.R. Kaufmann. 1973. The osmotic potential of polyethylene glycol 6000. Plant Physiol. 51: 914-916. Miransari, M., and D.L. Smith. 2014. Plant hormones and seed germination. Environ. Exp. Bot. 99: 110– 121. Nakano, Y., and K. Asada. 1981. Hydrogen peroxide scavenged by ascrobate-specific peroxidase in spinach chloroplast. Plant Cell Physiol. 22: 867–880. Piterkova, J., L. Luhova, J. Hofman, V. Turec, O. Nova, M. Petrivalsky, and M. Fellner. 2012. Nitric oxide is involved in light-specific responses of tomato during germination under normal and osmotic stress conditions. Ann. Bot. 110: 767–776. Qiao, W., C. Li, and L. M. Fan. 2014. Cross-talk between nitric oxide and hydrogen peroxide in plant responses to abiotic stresses. Environ. Exp. Bot. 100: 84-93. Rahnama-Ghahfarokhi, A., and R. Tavakkol-Afshari. 2007. Methods for dormancy breaking and germination of galbanum seeds (Ferula gummosa). Asian J. Plant Sci. 6: 611-616. Rehman, H., H. Iqbal, S.M.A. Basra, I. Afzal, M. Farooq, A. Wakeel, and W. Ning. 2015. Seed priming improves early seedling vigor, growth and productivity of spring maize. J. Integ. Agric. 14(9): 1745–1754. Sarath, G., G. Hou, L.M. Baird, and R.B. Mitchell. 2007. Reactive oxygen species, ABA and nitric oxide interactions on germination of warm-season C4-grasses. Planta. 226: 697–708. Sedghi, M., A. Gholipour, and R. Seyyed Sharifi. 2008.γ-tocopherol accumulation and floral differentiation of medicinal pumpkin (Cucurbıta pepo L.) in response to plant growth regulators. Not. Bot. Hortic. Agrobot. 36 (1): 80-84. Sheokand, S., V. Bhankar, and V. Sawhney. 2010.Ameliorative effect of exogenous nitric oxide on oxidative metabolism in NaCl treated chickpea plants.Brazilian J. Plant Physiol. 22(2):81-90. Sirova, J., M. Sedlaova, J. Piterkova, L. Luhova, and M. Petrivalsky. 2011. The role of nitric oxide in the germination of plant seeds and pollen. Plant Sci.181:560–572. Tabatabaei, S.A. 2013. The Effect of priming on germination and enzyme activity of sesame (Sesamum indicum L.) seeds after accelerated aging. J. Physiol. Biochem. 9 (4): 132-138. Tanou, G., C. Job, L. Rajjou, E. Arc, M. Belghazi, G. Diamantidis, A. Molassiotis, and D. Job. 2009. Proteomics reveals the overlapping roles of hydrogen peroxide and nitric oxide in the acclimation of citrus plants to salinity. Plant J. 60:795–804. Tian, Q. Y., D. H. Sun, M. G. Zhao, and W. H. Zhang. 2007. Inhibition of nitric oxide synthase (NOS) underline alaminium-induced inhibition of root elongation in Hibiscus moscheutos. New Physiol. 174: 322-331 Varier, A., A.K., Vari, and M. Dadlani. 2010. The subcellular basis of seed priming. Current Sci. 99(4): 450-456. Wu, X., W. Zhu, H. Zhang, H. Ding, and H.J. Zhang. 2011. Exogenous nitric oxide protects against salt induced oxidative stress in the leaves from two genotypes of tomato (Lycopersicon esculentum Mill.). Acta Physiol. Planta. 33: 1199–1209. Xia, F., X.Wang, M. Li, and P. Mao. 2015. Mitochondrial structural and antioxidant system responses to aging in oat (Avena sativa L.) seeds with different moisture contents. Plant Physiol. Biochem. 94: 122-129. Yan, M. 2015. Hydropriming promotes germination of aged napa cabbage seeds. Seed Sci. Technol. 43(2): 303-307. Yin, X., D. He, R. Gupta, and P. Yang. 2015. Physiological and proteomic analyses on artificially aged Brassica napus seeds. Front. Plant Sci. 6: 1-11. Zanardo, D. I., F. M. Zanardo, M. de Lourdes Ferrarese, J. R. Magalhaes, and O. Ferrarese-Filho. 2005. Nitric oxide affecting seed germination and peroxidase activity in canola (Brassica napus L.). Physiol. Mol. Biol. Plants. 11(1): 81. Zhang, L., Y. Zhao, Y. Zhai, M. Gao, X. Zhang, K. Wang, W. Nan, and J. Liu. 2012. Effects of exogenous nitric oxide on glycinebetaine metabolism in maize (Zea mays L.) seedlings under drought stress. Pak. J. Bot. 44(6): 1837-1844. Zheng, C., D. Jiang, F. Liu, T. Dai, W. Liu, Q. Jing, and W. Cao. 2009. Exogenous nitric oxide improves seed germination in wheat against mitochondrial oxidative damage induced by high salinity. Environm. Exp. Bot. 67(1): 222-227. | ||
|
آمار تعداد مشاهده مقاله: 659 تعداد دریافت فایل اصل مقاله: 501 |
||