| تعداد نشریات | 284 |
| تعداد نشریات سازمان | 82 |
| تعداد شماره‌ها | 3,081 |
| تعداد مقالات | 34,377 |
| تعداد مشاهده مقاله | 48,034,600 |
| تعداد دریافت فایل اصل مقاله | 78,939,829 |
Restriction Fragment Length Polymorphism Typing of Staphylococcus aureus Strains Isolated from Bovine Mastitis and Dairy Products in Ahvaz, Iran, Using of Digested Coagulase Gene | ||
| Archives of Razi Institute | ||
| مقاله 11، دوره 74، شماره 3، آذر 2019، صفحه 303-311 اصل مقاله (206.66 K) | ||
| نوع مقاله: Short Communication | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2017.115900.1159 | ||
| نویسندگان | ||
| D. Gharibi1؛ R. Ghadimipour* 1، 2؛ M. Ghorbanpoor1؛ A. Fazlara3 | ||
| 1Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran | ||
| 2Department of Research and Development, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization , Ahvaz, Iran | ||
| 3Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran | ||
| چکیده | ||
| Staphylococcus aureus is a major pathogen in the transmission of diseases from animals to humans and vice-versa.Various infections, such as mastitis in cattle, sheep and goats, as well as gastroenteritis due to food poisoning in humans are the most frequent problems caused by S. aureus. The bacteria also lead to severe economic losses in dairy industry. A major virulence factor for the organism is encoded by the coagulase (coa) gene. This study aimed to assess the polymorphisms of the coa gene in S. aureus strains isolated from bovine mastitis and dairy product samples in Ahvaz, Iran. The results showed that out of 91 S. aureus, 80 (87.91%) isolates were positive for coa gene(s). In total, nine different polymerase chain reaction (PCR) products were obtained for coa-positive isolates. A single band was detected in coa PCR with a size ranges from 370 to 830 bp in most isolates (n=77, 96.25%). For three isolates (3.75%), two amplification products were obtained. A PCR product of an estimated size of 590 bp was most frequent, as obtained for 48 (60.00%) isolates. Whereas, 370 and 830 bp PCR products were the least presented, for two (2.50%) and one (1.25%) isolate, respectively. Subsequently, for restriction fragment length polymorphism (RFLP), typing of coa gene and AluI restriction enzyme were used for the digestion of the products. AluI for most of PCR products generated a unique pattern; however, four PCR products (the sizes ranged 750, 670, 590, and 510 bp) generated three or more patterns. Based on AluI RFLP of coa gene, the isolates were classified into 23 groups. Two groups of isolates were dominant, making 45% of the total. According to the findings, one or two types of coa RFLP were dominant among samples that were infected with more S. aureus isolates belonging to different coa RFLP types. | ||
| کلیدواژه‌ها | ||
| Staphylococcus aureus؛ Coagulase Gene؛ Polymerase Chain Reaction؛ Restriction Fragment Length Polymorphism؛ Bovine Mastitis؛ Dairy Products؛ Iran | ||
| عنوان مقاله [English] | ||
| تایپینگ سویه‌های استافیلوکوکوس اورئوس جداشده از موارد ورم پستان گاوی و محصولات لبنی شهر اهواز با بررسی پلی‌موفیسم طول قطعات حاصل از هضم ژن کواگولاز | ||
| چکیده [English] | ||
| استافیلوکوکوس اورئوس یکی از عوامل بیماری‌زای شناخته‌شده حیوانات و انسان به شمار می‌رود. عفونت‌های مختلفی مانند ورم پستان در گاو، گوسفند و بز، و نیز مسمومیت غذایی و گاستروانتریت در انسان از مشکلات شایعی هستند که توسط این باکتری ایجاد می‌شوند. استافیلوکوکوس اورئوس همچنین منجر به خسارت‌های شدید اقتصادی در صنایع لبنی می‌شود. ژن کواگولاز (coa) یکی از مهمترین فاکتورهای حدت این ارگانیسم محسوب می‌شود. مطالعه حاضر با هدف بررسی پلی‌مورفیسم این ژن در جدایه‌های استافیلوکوکوس اورئوس حاصل از موارد ورم پستان گاوی و محصولات لبنی شهر اهواز در جنوب غرب ایران طراحی گردید. آزمایش مولکولی 91 جدایه تحت مطالعه نشان داد که 80 جدایه (91/87%) واجد ژن کواگولاز میباشند. در مجموع نه الگوی واکنش زنجیرهای پلیمراز از تکثیر ژن کواگولاز این جدایه‌ها به دست آمد که الگوی اکثر جدایه‌ها(n=77, 96.25%) شامل یک باند 830-370 جفت بازی (bp) بود، در حالی که تعداد کمی از آن‌ها (n=3, 3.75%) محصول واکنش زنجیرهای پلیمراز دو باندی را نشان دادند. یافته‌های ما همچنین مشخص نمود که 48 جدایه (00/60%) باندی با اندازه تقریبی 590 جفت باز را تولید کردند، ولی محصولات 370 و 830 جفت بازی به ترتیب فقط برای دو (50/2%) و یک جدایه (25/1%) تشخیص داده شدند. در ادامه بررسی‌ها از آنزیم محدود‌الاثر AluI برای هضم محصولات واکنش زنجیرهای پلیمراز تکثیر ژن کواگولاز استفاده گردید. بر اساس این آزمایش‌ها برخی از محصولات واکنش زنجیرهای پلیمراز فقط یک پروفایل تولید کردند، در صورتی که چهار محصول 750، 670، 590 و 510 جفت بازی منجر به ایجاد سه و یا تعداد بیشتری پروفایل گردیدند. بطور کلی با ارزیابی پلی‌مورفیسم طول قطعات حاصل از هضم (RFLP)، تنوع بالایی در ژن مورد مطالعه مشاهده شده و جدایه‌ها در 23 پروفایل مختلف طبقه‌بندی گردیدند، با این همه در مجموع 45% آن‌ها متعلق به دو پروفایل بودند. بر اساس یافته‌های این مطالعه اگر چه نمونه‌های مورد آزمایش آلوده به سویه‌هایی از استافیلوکوکوس اورئوس بودند که از نظر پلی‌مورفیسم ژن کواگولاز به ژنوتیپ‌های متعددی تعلق داشتند ولی اغلب این جدایه‌ها به یک یا دو ژنوتیپ غالب مربوط می‌شدند. | ||
| مراجع | ||
|
Aarestrup, F.M., Dangler, C.A., Sordillo, L.M., 1995. Prevalence of coagulase gene polymorphism in Staphylococcus aureus isolates causing bovine mastitis. Can J Vet Res 59, 124-128.
Abdulghany, H.M., Khairy, R.M., 2014. The Frequency of methicillin-resistant Staphylococcus aureus and coagulase gene polymorphism in Egypt. Int J Bacteriol 2014.
Coelho, S.M., Reinoso, E., Pereira, I.A., Soares, L.C., Demo, M., Bogni, C., et al., 2009. Virulence factors and antimicrobial resistance of Staphylococcus aureus isolated from bovine mastitis in Rio de Janeiro. Pesqui Vet Bras 29, 369-374.
Da Silva, E., Boechat, J., Da Silva, N., 2006. Coagulase gene polymorphism of Staphylococcus aureus isolated from goat mastitis in Brazilian dairy herds. Lett Appl Microbiol 42, 30-34.
Dallal, M.M.S., Khoramizadeh, M.R., Amiri, S.A., Yaraghi, A.A.S., Fard, R.M.N., 2016. Coagulase gene polymorphism of Staphylococcus aureus isolates: A study on dairy food products and other foods in Tehran, Iran. Food Sci Hum Wellness 5, 186-190.
Goh, S.H., Byrne, S.K., Zhang, J.L., Chow, A.W., 1992. Molecular typing of Staphylococcus aureus on the basis of coagulase gene polymorphisms. J Clin Microbiol 30, 1642-1645.
Gould, I.M., 2009. Antibiotics, skin and soft tissue infection and meticillin-resistant Staphylococcus aureus: cause and effect. Int J Antimicrob Agents 34, S8-S11.
Güler, L., Ok, Ü., Gündüz, K., Gülcü, Y., Hadimli, H., 2005. Antimicrobial susceptibility and coagulase gene typing of Staphylococcus aureus isolated from bovine clinical mastitis cases in Turkey. J Dairy Sci 88, 3149-3154.
Hasman, H., Moodley, A., Guardabassi, L., Stegger, M., Skov, R., Aarestrup, F.M., 2010. Spa type distribution in Staphylococcus aureus originating from pigs, cattle and poultry. Vet Microbiol 141, 326-331.
Hennekinne, J.-A., De Buyser, M.-L., Dragacci, S., 2012. Staphylococcus aureus and its food poisoning toxins: characterization and outbreak investigation. FEMS Microbiol Rev 36, 815-836.
Himabindu, M., Muthamilselvan, D.S., Bishi, D.K., Verma, R.S., 2009. Molecular analysis of coagulase gene polymorphism in clinical isolates of methicillin resistant Staphylococcus aureus by restriction fragment length polymorphism based genotyping. Am J Infect Dis 5, 170-176.
Hookey, J.V., Richardson, J.F., Cookson, B.D., 1998. Molecular typing of Staphylococcus aureus based on PCR restriction fragment length polymorphism and DNA sequence analysis of the coagulase gene. J Clin Microbiol 36, 1083-1089.
Janwithayanuchit, I., Ngam-Ululert, S., Paungmoung, P., Rangsipanuratn, W., 2006. Epidemiologic study of methicillin-resistant Staphylococcus aureus by coagulase gene polymorphism. Sci Asia 32, 127-132.
Karahan, M., Cetinkaya, B., 2007. Coagulase gene polymorphisms detected by PCR in Staphylococcus aureus isolated from subclinical bovine mastitis in Turkey. Vet J 174, 428-431.
Khichar, V., Kataria, A.K., Sharma, R., 2014. Characterization of Staphylococcus aureus of cattle mastitis origin for two virulence-associated genes (coa and spa). Comp Clin Path 23, 603-611.
Markey, B., Leonard, F., Archambault, M., Cullinane, A., Maguire, D., 2013. Clin Vet Microbiol E-Book, Elsevier Health Sciences.
Mehndiratta, P., Bhalla, P., Ahmed, A., Sharma, Y., 2009. Molecular typing of methicillin-resistant Staphylococcus aureus strains by PCR-RFLP of SPA gene: a reference laboratory perspective. Indian J Med Microbiol 27, 116.
Momtaz, H., Tajbakhsh, E., Rahimi, E., Momeni, M., 2011. Coagulase gene polymorphism of Staphylococcus aureus isolated from clinical and sub-clinical bovine mastitis in Isfahan and Chaharmahal va Bakhtiari provinces of Iran. Comp Clin Pathol 20, 519-522.
Moon, J.S., Lee, A.R., Jaw, S.H., Kang, H.M., Joo, Y.S., Park, Y.H., et al., 2007. Comparison of antibiogram, staphylococcal enterotoxin productivity, and coagulase genotypes among Staphylococcus aureus isolated from animal and vegetable sources in Korea. J Food Protec 70, 2541-2548.
Morandi, S., Brasca, M., Lodi, R., Brusetti, L., Andrighetto, C., Lombardi, A., 2010. Biochemical profiles, restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD) and multilocus variable number tandem repeat analysis (MLVA) for typing Staphylococcus aureus isolated from dairy products. Res Vet Sci 88, 427-435.
Mullarky, I., Su, C., Frieze, N., Park, Y., Sordillo, L., 2001. Staphylococcus aureus agr genotypes with enterotoxin production capabilities can resist neutrophil bactericidal activity. Infect Immun 69, 45-51.
Okolie, C.E., Wooldridge, K.G., Turner, D.P., Cockayne, A., James, R., 2015. Development of a new pentaplex real-time PCR assay for the identification of poly-microbial specimens containing Staphylococcus aureus and other staphylococci, with simultaneous detection of staphylococcal virulence and methicillin resistance markers. Mol Cell Probes 29, 144-150.
Rabello, R.F., Moreira, B.M., Lopes, R.M.M., Teixeira, L.M., Riley, L.W., Castro, A.C.D., 2007. Multilocus sequence typing of Staphylococcus aureus isolates recovered from cows with mastitis in Brazilian dairy herds. J Med Microbiol 56, 1505-1511.
Raimundo, O., Deighton, M., Capstick, J., Gerraty, N., 1999. Molecular typing of Staphylococcus aureus of bovine origin by polymorphisms of the coagulase gene. Vet Microbiol 66, 275-284.
Rodrigues da Silva, E., da Silva, N., 2005. Coagulase gene typing of Staphylococcus aureus isolated from cows with mastitis in southeastern Brazil. Can J Vet Res 69, 260-264.
Saei, H.D., Ahmadi, M., 2012. Discrimination of Staphylococcus aureus isolates on the basis of gene coding protein A using PCR-restriction enzyme analysis. Comparative Clin Pathol 21, 645-652.
Saei, H.D., Ahmadi, M., Mardani, K., Batavani, R.A., 2009. Molecular typing of Staphylococcus aureus isolated from bovine mastitis based on polymorphism of the coagulase gene in the north west of Iran. Vet Microbiol 137, 202-206.
Shin, E., Hong, H., Park, J., Oh, Y., Jung, J., Lee, Y., 2016. Characterization of Staphylococcus aureus faecal isolates associated with food-borne disease in Korea. Journal of Applied Microbiology 121, 277-286.
Shopsin, B., Kreiswirth, B.N., 2001. Molecular epidemiology of methicillin-resistant Staphylococcus aureus. Emerg Infect Dis 7, 323-326.
Su, C., Herbelin, C., Frieze, N., Skardova, O., Sordillo, L.M., 1999. Coagulase gene polymorphism of Staphylococcus aureus isolates from dairy cattle in different geographical areas. Epidemiol Infect 122, 329-336.
Suleiman, A., Kwaga, J., Umoh, V., Okolocha, E., Muhammed, M., Lammler, C., et al., 2012. Macro-restriction analysis of Staphylococcus aureus isolated from subclinical bovine mastitis in Nigeria. Afr J Microbiol Res 6, 6270-6274.
Tsegmed, U., Normanno, G., Pringle, M., Krovacek, K., 2007. Occurrence of enterotoxic Staphylococcus aureus in raw milk from yaks and cattle in Mongolia. J Food Prot 70, 1726-1729.
Umesha, S., Manukumar, H.M., 2018. Advanced molecular diagnostic techniques for detection of food-borne pathogens: Current applications and future challenges. Crit Rev Food Sci Nutr 58, 84-104.
Zadoks, R., Van Leeuwen, W., Kreft, D., Fox, L., Barkema, H., Schukken, Y., et al., 2002. Comparison of Staphylococcus aureus isolates from bovine and human skin, milking equipment, and bovine milk by phage typing, pulsed-field gel electrophoresis, and binary typing. J Clin Microbiol 40, 3894-3902. | ||
|
آمار تعداد مشاهده مقاله: 1,459 تعداد دریافت فایل اصل مقاله: 2,556 |
||