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Determination of the Effective Dose of Curcumin alone and in Combination with Antimicrobial Peptide CM11 on Promastigote Forms of Iranian Strain of L. major (MRHO / IR / 75 / ER) | ||
| Archives of Razi Institute | ||
| مقاله 11، دوره 74، شماره 4، اسفند 2019، صفحه 413-422 اصل مقاله (851.86 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2018.122300.1222 | ||
| نویسندگان | ||
| G. Aqeele1، 2؛ P. Shayan* 1، 2، 3؛ E. Ebrahimzadeh1، 4؛ M. Mohebali5؛ S. Khalili1 | ||
| 1Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran | ||
| 2Department of Microbiology, Faculty of Medicine, University of Wasit, Iraq | ||
| 3Institute Molecular Biological System Transfer (MBST) | ||
| 4Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran | ||
| 5Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran | ||
| چکیده | ||
| Zoonotic cutaneous leishmaniasis t caused by Leishmania major is spread in focal areas of more than 90 countries in the tropics, subtropics, and southern Europe. In the absence of any effective vaccine, the only means to treat and control leishmaniasis is conventional medication. Glucantime is the first choice of anti-leishmanialdrug, has serious side effects like high toxicity, exorbitant cost, problems with the administration and development of resistance. Curcumin is the active component from the rhizome of herb Curcuma longa, possessing many pharmacological and biological activities with antiprotozoal and anti-proliferative effects which make it a good alternative to existing therapy. Antimicrobial peptides like CM11, a small peptide consisting of 11 amino acids, are also novel potential drugs against at least wide spectrum of microbial organisms. The aim of this study was to evaluate the effect of curcumin alone and in combination with CM11 on promastigote form of L. major (MRHO / IR / 75 / ER) for 12h and 24h in vitro. The results of Giemsa staining showed that the morphology of the flagellum and cell shape increased changed with increasing concentration of curcumin (5 µM, 10 μM, 20 μM, 40 μM and 80 μM). MTT and Trypan blue results demonstrated that the promastigotes were susceptible against curcumin in dose and time dependent manner, while CM11 alone at concentration of 8 µM as well as in combination with 10 and 20 µM curcumin had no significant effect on promastigotes. Our results revealed that curcumin can provide a new curative candidate against cutaneous leishmaniasis. | ||
| کلیدواژه‌ها | ||
| Curcumin؛ Antimicrobial Peptide CM11؛ Promastigote؛ Leishmania. major؛ Glucantime | ||
| عنوان مقاله [English] | ||
| تعیین تاثیر کورکومین به تنهایی و به همراه پپتید ضد باکتریایی CM11 بر روی پروماستیگوت لیشمانیا ماژور سویه ایران (MRHO/IR/75/ER) | ||
| چکیده [English] | ||
| ابتلا به لیشمانیوز جلدی در بیشتر از 90 کشور گرم سیر، نیمه گرمسیر و اروپای جنوبی قابل مشاهده میباشد. در غیاب واکسن موثر، تنها راه مبارزه و کنترل لیشمانیوز از طریق معالجه معمول دارویی میباشد. داروی گلوکانتیم که به عنوان داروی انتخاب اول بر ضد لیشمانیوز مورد استفاده قرار میگیرد، از معایب و آثار جانبی زیادی از قبیل سمی بودن و ایجا د مقاومت دارویی برخوردار است. کورکومین جزء فعال زردچوبه میباشد و دارای فعالیت های فارماکولوژیک و بیولوژیک از قبیل آنتی میکروبیال و ضد تکثیر سلولی میباشد، و این خصوصیات آن را به عنوان آلنترناتیو خوبی برای مبارزه با میکروبها قرار میدهد. پپتیدهای ضد باکتریایی از قبیل CM11 که یک پپتید کوچک با 11 اسید آمینه میباشد، همچنین میتوانند به عنوان داروهایی بر علیه طیف وسیعی از میکرواورگانیسمها مورد استفاده قرار گیرند. هدف از مطالعه حاضر ارزیابی تاثیر کورکومین به تنهایی و به همراه پپتید CM11 بر روی شکل پروماستیگوت انگل لیشمانیا بود. در رنگ آمیزی گیمسا نشان داده شد که کورکومین وابسته به غلظت (5 µM, 10 μM, 20 μM, 40 μM and 80 μM) بر روی موفولوژی تاژک و شکل انگل تاثیر میگذارد. نتایج MTT و رنگ آمیزی Trypan blue نشان دادند که فرم پروماستیگوت انگل در مقابل کوکومین وابسته به غلظت حساس بود در حالی که حساسیت ملموسی در مقابل پپتید CM11 به تنهایی در غلظت 8 میکرومولار و یا به همرا کورکومین در غلظتهای 10 و 20 میکرومولار نشان نداد. نتایج این مطاله نشان میدهند که کورکومین میتواند به عنوان کاندید بر علیه لیشمانیای جلدی مورد ارزیابی قرار گیرد. | ||
| کلیدواژه‌ها [English] | ||
| کورکومین, آنتی باکتریال پپتید CM1, پروماستیگوت, لیشمانیا ماژور, گلوکانتیم | ||
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