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مطالعه الگوی بیان MicroRNAهای دخیل در تحمل تنش خشکی در اجداد و خویشاوندان وحشی و اهلی گندم | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| مقاله 2، دوره 27، شماره 1 - شماره پیاپی 53، شهریور 1398، صفحه 1-14 اصل مقاله (1.01 M) | ||
| نوع مقاله: مقاله علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2019.120248 | ||
| نویسندگان | ||
| بتول شهیدی1؛ جعفر احمدی2؛ صدیقه فابریکی اورنگ* 3 | ||
| 1دانشجوی کارشناسی ارشد ژنتیک و به‌نژادی گیاهی، دانشگاه بین‌المللی امام خمینی (ره)، قزوین، ایران | ||
| 2استاد، هیئت علمی گروه ژنتیک و به‌نژادی گیاهی، دانشگاه بین‌المللی امام خمینی (ره)، قزوین، ایران | ||
| 3استادیار، هیات علمی دانشگاه بین‌المللی امام خمینی (ره)، قزوین، ایران | ||
| چکیده | ||
| DOR: 98.1000/1735-0891.1398.27.1.53.1.1605.1606 miRNA ها، مولکول‌های کوچک غیرکدکننده هستند که بیان ژن‌ها را از طریق هضم mRNAیا ممانعت از ترجمه تنظیم می‌کنند. این مولکول‌ها با تنظیم دقیق بیان ژن‌ها، امکان پاسخ مناسب گیاه را به تغییرات مراحل رشدی یا شرایط محیطی فراهم می‌کنند. در این تحقیق با استفاده از تکنیک Real Time-PCR الگوی بیان miRNA159، miRNA160 و miRNA398 به همراه ژن هدف miRNA398 (CSD) در شش گونه متعلق به جنسهای تریتیکوم و آژیلوپس در کنار دو رقم گندم نان شاهد حساس (دریا) و متحمل به خشکی (سیروان)، در شرایط عدم تنش (FC=100%) و تنش خشکی (FC=25%) مورد بررسی قرار گرفتند. تجزیه qRT-PCR نشان داد که تحت تنش خشکی هر سه miRNA افزایش بیان معنی داری (P≤0.01) نسبت به شرایط عدم تنش داشتند. در بین گونه‌های مورد بررسی بیشترین میزان بیان miRNA159 در گونه Ae.tauschii، miRNA160 در گونه T.urartu، miRNA398 در گونه T.durum و ژن CSD در گونه T.durum مشاهده شد. در مقایسه میانگین اثر متقابل گونه و تنش بیشترین درصد افزایش بیان برای هر سه miRNA در شرایط تنش خشکی در سه گونه T.durum، T.urartu و Ae.tauschii مشاهده شد. همچنین بیشترین میزان افزایش بیان CSD در گونه‌های T.urartu، T.durum و Ae.tauschii به‌ترتیب با 62/6، 21/6 و 038/6 برابر نسبت به شرایط عدم تنش مشاهده گردید. در جمع‌بندی مشخص شد که سه گونه T.urartu، T.durum و Ae.tauschii به دلیل برتری از لحاظ miRNAهای عامل ایجاد تحمل میتوانند کاندیدهای مناسبی برای غنی سازی ژرم‌پلاسم گندم نان و اصلاح برای تحمل تنش خشکی باشند. | ||
| کلیدواژه‌ها | ||
| گندم وحشی؛ تنش خشکی؛ miRNA؛ PCR در زمان واقعی | ||
| عنوان مقاله [English] | ||
| The Study of microRNAs expression pattern involved in drought stress tolerance in ancestors and wild-domestic relatives of wheat | ||
| نویسندگان [English] | ||
| B. Shahidi1؛ J. Ahmadi2؛ Sedigheh Fabriki-Ourang3 | ||
| 1M.Sc. Student of Genetics and Plant Breeding, Imam Khomeini International University, Qazvin, Iran. | ||
| 2Professor, Department of Genetics and Plant Breeding, Imam Khomeini International University, Qazvin, Iran | ||
| 3Assistant Professor, Department of Production and Plant Breeding, Imam Khomeini International University, Qazvin, Iran | ||
| چکیده [English] | ||
| DOR: 98.1000/1735-0891.1398.27.1.53.1.1605.1606 MicroRNAs are small non-coding molecules that regulate the expression of genes through mRNA digestion and/or translation inhibition. By accurately regulation the expression of the genes, these molecules allow the plant to respond appropriately to the changes in growth stages or environmental conditions. In this study, the miRNA159, miRNA160, miRNA398, and the miRNA398 targeted gene (CSD) were investigated in six species belonging to the Triticum and Aegilops species, along with the susceptible (DARYA) and tolerant (SIRVAN) check cultivars under drought (FC=25%) and non-stress (FC = 100%) conditions using real-time PCR. The qRT-PCR analysis showed that all three miRNAs had a significant (P≤0.01) increase in expression under drought stress. The highest expression of miRNA159, miRNA160, miRNA398, and CSD gene was observed in the species Ae.tauschii, T.urartu, T.durum, and T.durum, respectively. By the mean comparing of species × stress interaction, the highest expressions were observed in the three species T.durum, T.urartu and Ae.tauschii for all three miRNAs under drought stress. Also, the highest increase in CSD expression under drought stress was observed in T.urartu, T.durum and Ae.tauschii equal to 6.62, 6. 21 and 0.63 fold in compared to the non-stress condition, respectively. It was concluded that the species T.urartu, T.durum and Ae.tauschii due to their superiority in terms of containing miRNAs causer tolerance, could be suitable candidates for bread wheat germplasm enrichment and breeding to drought stress tolerance. | ||
| کلیدواژه‌ها [English] | ||
| Drought Stress, MiRNA, Real-time PCR, Wild Wheat | ||
| مراجع | ||
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