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بررسی اثر تابش دزهای پایین پرتوی گاما به تنهایی و یا همراه با اسید سالیسیلیک و حرارت غیرمستقیم بر آلودگی بذرزاد ویروس موزاییک معمولی لوبیا در گیاه لوبیا | ||
| آفات و بیماری های گیاهی | ||
| مقاله 8، دوره 87، شماره 1 - شماره پیاپی 108، شهریور 1398، صفحه 87-105 اصل مقاله (1.13 M) | ||
| نوع مقاله: بیماری‌شناسی گیاهی | ||
| شناسه دیجیتال (DOI): 10.22092/jaep.2019.124059.1251 | ||
| نویسندگان | ||
| فرشاد رخشنده رو* 1؛ حمیده مردانی مهرآباد2؛ سمیرا شهبازی3؛ نوح شهرآئین4 | ||
| 1عضو هیئت علمی و مدیر گروه تخصصی، گروه گیاهپزشکی، دانشکده علوم کشاورزی وصنایع غذایی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران | ||
| 2گروه گیاهپزشکی، دانشکده علوم کشاورزی وصنایع غذایی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران | ||
| 3گروه گیاهپزشکی، دانشکده تحقیقات کشاورزی هسته‌ای، موسسه تحقیقات علوم و فناوری هسته‌ای (NSTRI)، سازمان انرژی اتمی ایران (AEOI)، البرز، ایران. | ||
| 4بخش تحقیقات ویروس شناسی، موسسه تحقیقات گیاهپزشکی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران | ||
| چکیده | ||
| در این پژوهش تأثیر دزهای پایین پرتوی گاما (10، 20 و 30 گری) به‌تنهایی و همچنین همراه با اسید سالیسیلیک و حرارت غیرمستقیم بر ویروس بذرزاد موزاییک معمولی لوبیا (BCMV)، بررسی شد. بذور لوبیای رقم تجاری "صدری" دارای آلودگی بذرزاد به ویروس BCMV کشت شدند. گیاهچههای آلوده و عاری از ویروس بر اساس آزمون الایزای غیرمستقیم (PTA-ELISA) تفکیک و از آنها بذرگیری شد. نتایج نشان داد که شاخصهای رشدی در گیاهچه های آلوده و عاری از ویروس (شاهد) تیمار شده با تابش گاما به تنهایی و نیز همراه با اسید سالیسیلیک و یا حرارت غیرمستقیم در مقایسه با گیاهچههایی که پرتوتابی نشدهاند در سطح احتمال 5 درصد افزایش معنیداری یافته است. پرتوتابی گاما موجب القاء فعالیت اختصاصی آنزیم های آنتیاکسیدان (PPO، POX، CAT و PAL) و همچنین افزایش چگالی پروتئین در گیاهچههای آلوده و سالم و کاهش میزان غلظت پروتئینهای BCMV در گیاهچههای آلوده تیمار شده با پرتو گاما به تنهایی و نیز همراه با اسید سالیسیلیک و یا حرارت غیرمستقیم در مقایسه با گیاهچه های پرتو ندیده شد. نتایج این پژوهش بیانگر القاء مقاومت نسبی در گیاهچه های دارای آلودگی بذرزاد به BCMV تیمار شده با دزهای 20 و 30 گری پرتوی گاما به تنهایی و نیز همراه با اسید سالیسیلیک و حرارت غیرمستقیم بود. | ||
| کلیدواژه‌ها | ||
| اسید سالیسیلیک؛ الگوی پروتئینی؛ پرتوتابی گاما؛ فعالیت آنزیم های آنتی اکسیدان؛ BCMV | ||
| عنوان مقاله [English] | ||
| Study on the effect of low doses of gamma irradiation alone or in associated with the salicylic acid and indirect temperature on seed borne infection of Bean common mosaic virus in bean plant | ||
| نویسندگان [English] | ||
| Farshad Rakhshandehroo1؛ Hamideh Mardani-Mehrabad2؛ samira shahbazi3؛ Nooh Shahraeen4 | ||
| 1College of Agricultural Sciences and Food Technologies , Science and Research Branch, Islamic Azad university, Tehran, Iran | ||
| 2Department of Plant Protection, College of Agricultural Sciences and Food Technologies , Science and Research Branch, Islamic Azad university, Tehran, Iran | ||
| 32. Plant Protection Department, Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute (NSTRI), Atomic Energy Organization of Iran (AEOI), Alborz, Iran. | ||
| 4Research Department of Plant Virology, Iranian Research Institute of Plant Protection (IRIPP), Tehran, Iran | ||
| چکیده [English] | ||
| In this study, the effect of the low doses of gamma irradiation (10, 20, and 30 Gy) alone and in associated with the salicylic acid and indirect temperature on the seed borne infection of Bean common mosaic virus (BCMV) was assessed. Commercial bean seeds called “Sadri” with seed borne infection to BCMV were sowed. The seedlings were tested for the infection of BCMV by indirect plate-trapped antigen (PTA- ELISA) test and seeded. Results indicated that the growth factors in the irradiated seeds alone and in associated with the salicylic acid or indirect temperature significantly increased (P≤0.05) in healthy and infected seedlings compared to the non-irradiated seedlings. Gamma irradiation induced the specific activity of various antioxidant enzymes (PPO, POX, CAT and PAL) as well as the total protein density in treated healthy and infected beans, and decreased the concentration of BCMV proteins in the infected and irradiated seeds alone and so in associated with the salicylic acid or indirect temperature compared to the non-irradiated seedlings. Results of this research indicated to the induction of the relative resistance against BCMV in infected bean seedlings treated with the 20 and 30 Gy doses of gamma irradiation alone or in associated with the salicylic acid and indirect temperature. | ||
| کلیدواژه‌ها [English] | ||
| Keywords: Antioxidant enzymes activity, BCMV, gamma irradiation, protein pattern, salicylic acid | ||
| مراجع | ||
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