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شناسایی مولکولی ویروس پیسک توتفرنگی (Strawberry mottle virus) از مزارع توتفرنگی استان کردستان بر اساس منطقه 3΄NCR | ||
| آفات و بیماری های گیاهی | ||
| مقاله 9، دوره 87، شماره 1 - شماره پیاپی 108، شهریور 1398، صفحه 107-116 اصل مقاله (697.3 K) | ||
| نوع مقاله: بیماریشناسی گیاهی | ||
| شناسه دیجیتال (DOI): 10.22092/jaep.2019.125077.1266 | ||
| نویسندگان | ||
| نسرین قادری زندان1؛ محمد حاجی زاده* 2 | ||
| 1گروه گیاهپزشکی دانشگاه کردستان، سنندج، ایران | ||
| 2هیات علمی دانشگاه کردستان | ||
| چکیده | ||
| در تابستان 1397، علائم ویروسی شامل پیسک، بدشکلی و سوختگی حاشیه برگها از مزارع توتفرنگی استان کردستان مشاهده و تعداد 48 نمونه برگی جمعآوری شد. ابتدا برخی نمونهها به گیاه محک Fragaria vesca با استفاده از شته توتفرنگی (Chaetosiphon fragaefolii) تلقیح و علایم مطابق با Strawberry mottle virus (SMoV) ظاهر شد. سپس، آر ان ا کل از نمونهها با روش سیلیکا استخراج و دی ان ا مکمل آنها با آغازگرهای تصادفی ششتایی ساخته شد. PCR با آغازگرهای اختصاصی SMoV- Sm2ncr1a و SMoV-Smdetncr4a انجام و قطعهای به طول 461 جفت باز در 28 نمونه از 48 نمونه فوق تکثیر گردید. بهمنظور اطمینان از نتیجه RT-PCR و بررسی مشخصات مولکولی، پنج جدایه براساس منطقه جغرافیایی، رقم میزبان و نوع علایم انتخاب و پس از الحاق به پلاسمید pTG-19 در باکتری Escherichia coliانتقال و پلاسمیدهای نوترکیب همسانه شده تعیین توالی شدند. میانگین فاصله ژنتیکی در بین جدایههای در این تحقیق در سطح نوکلئوتیدی 002/0 ± 005/0 و با سایر جدایههای موجود در بانک ژن 008/0 ± 084/0 بود. مقایسه دوبه دوی توالیهای نوکلئوتیدی نشان داد که بیشترین و کمترین فاصله ژنتیکی بهترتیب میان جدایه K3 با جدایه NSper51 از کانادا (015/0 ± 093/0) و جدایه SQA یا AG با جدایه NB926 از کانادا (007/0 ± 019/0) وجود داشت.این اولین گزارش از شناسایی این ویروس در مزارع توتفرنگی استان کردستان میباشد. | ||
| کلیدواژهها | ||
| آلودگی ویروسی؛ تبارزایی؛ توت فرنگی؛ فاصله ژنتیکی | ||
| عنوان مقاله [English] | ||
| Molecular identification of Strawberry mottle virus in strawberry fields of Kurdistan Province based on the 3΄NCR | ||
| نویسندگان [English] | ||
| Nasrin Ghaderi Zandan1؛ Mohammad Hajizadeh2 | ||
| 1Plant Protection Department, University of Kurdistan, Sanandaj, Iran | ||
| چکیده [English] | ||
| In the summer of 2018, viral symptoms corresponded to Strawberry mottle virus (SMoV) in some strawberry fields were observed and 48 leaf samples/plants were collected from different fields from Kurdistan Province. In the preliminary test, some samples were inoculated to Fragaria vesca by strawberry aphid (Chaetosiphon fragaefolii) and SMoV-symptoms were observed after about two weeks. Then, total RNA of samples was extracted by silica-capture method and subjected to cDNA synthesize with random hexamer primers. RT-PCR was done by specific SMoV pair primer and amplified fragments were observed on 1.2% agarose gels. RT-PCR results were showed that 28 out of 48 samples were infected by SMoV. Based on the geographical origin, cultivar and symptoms, five isolates were selected, ligated to pTG-19 and transformed to Escherichia coli. Recombinant plasmids were sequenced and analyzed for molecular characterization and phylogenetic studies on SMoV 3΄NCR. Mean genetic distance between the isolates from Iran was 0.005 ± 0.002 but between these isolates and the other isolates available in GenBank was 0.084 ± 0.008. The highest and lowest genetic distance was found between K3 with NSper51 (0.093 ± 0.008) and SQA/AG with NB926 (0.019 ± 0.017) from Canada, respectively. This is the first report on occurrence of SMoV on strawberry in Kurdistan Province. | ||
| کلیدواژهها [English] | ||
| Genetic distance, phylogeny, strawberry, viral infectio | ||
| مراجع | ||
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