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بررسی تغییرات فیزیولوژیکی و بیوشیمیایی بذر ذرت(Zea mays L. )در شرایط زوال طبیعی و مصنوعی | ||
| علوم و فناوری بذر ایران | ||
| مقاله 6، دوره 8، شماره 2 - شماره پیاپی 16، بهمن 1398، صفحه 73-85 اصل مقاله (798.27 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22034/ijsst.2019.110591.1090 | ||
| نویسندگان | ||
| بیتا اسکویی* 1؛ آیدین حمیدی2؛ سامان شیدائی3؛ مریم دیوسالار4؛ حسین صادقی5 | ||
| 1موسسه تحقیقات ثبت و گواهی بذر و نهال | ||
| 2دانشیارپژوهش /موسسه تحقیقات ثبت و گواهی بذر و نهال، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران | ||
| 3موسسه تحقیقات ثبت و گواهی بذر و نهال، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران | ||
| 4کارشناس/ موسسه تحقیقات ثبت و گواهی بذر و نهال، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران | ||
| 5موسسه تحقیقات ثبت و گواهی بذر و نهال، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران. | ||
| چکیده | ||
| این آزمایش، سال 1394در آزمایشگاه مؤسسه‌تحقیقات‌ثبت‌و گواهی‌بذر‌و نهال کرج انجام‌گرفت. تیمارهای‌آزمایشی شامل محل-تکثیر‌‌بذر ذرت در دو شرایط اقلیمی کرج و مغان، چگونگی‌شرایط نگهداری‌‌بذر‌ذرت در دو انبارکنترل‌شده و شرایط‌هوای‌آزاد‌منطقه-مغان و نحوه ضدعفونی‌بذر در دو‌سطح با تامین شرایط‌ضدعفونی و بدون‌ضدعفونی بودند. آزمایش به‌صورت فاکتوریل‌در‌‌قالب‌طرح-کاملاً‌تصادفی در سه‌تکرار انجام‌‌شد بذرهای‌تکثیری در‌شرایط‌اقلیمی‌کرج قبل از آزمون‌‌پیری‌تسریع شده دارای 92%جوانه‌زنی و پس‌از-144ساعت در شرایط‌پیری‌‌تسریع‌‌شده، در انبار با شرایط‌کنترل‌‌شده حدود3%، وقتی در شرایط‌آب‌و‌هوایی‌مغان نگهداری‌شدند حدود6 % افت درصدجوانه‌زنی‌استاندارد نشان‌دادند. در‌حالی‌که شرایط‌مشابه در بذرهای‌تولیدی‌مغان به‌ترتیب منجر‌به‌کاهش18و 28درصدی‌شد. زمانی‌که بذرها در انبار‌کنترل‌شده‌نگهداری‌شدند حدود17 %محتوی‌پروتئین بیش‌تری نسبت به انبارداری در شرایط‌آب‌و‌هوایی‌مغان نشان‌دادند. میزان‌فعالیت‌کاتالاز در بذرهای تولیدی‌کرج از بذرهای تولیدی‌مغان بیش‌تر(14%) بود. ضدعفونی‌بذر بر میزان فعالیت‌آنزیم‌پراکسیداز(p | ||
| کلیدواژه‌ها | ||
| تغییرات آنزیمی؛ جوانه زنی؛ زوال بذر؛ ذرت | ||
| عنوان مقاله [English] | ||
| Study of physiological and biochemical changes of maize seed(Zea mays l.) in natural and artificial aging conditions | ||
| نویسندگان [English] | ||
| Bita Oskouei1؛ Aidin Hamidi2؛ Saman Sheidaei3؛ maryam divsalar4؛ hossein sadeghi5 | ||
| 1seed and plant certification and registration institute of Iran | ||
| 2seed and plant certification and registration institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran | ||
| 3seed and plant certification and registration institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran | ||
| 4seed and plant certification and registration institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran | ||
| 5seed and plant certification and registration institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran | ||
| چکیده [English] | ||
| This experiment was done in seed and plant certification and registration institute laboratory in 2015. The treatments were corn seed produced in Karaj and Moghan , seed storing condition in two controlled and Free weather conditions stores also Disinfection and non- Disinfection. The experiment was conducted as factorial based on completely randomized design in three replications. Germination percent of Karaj seeds before accelerated aging test was 92 percent and after 144 hours aging, it reduced 3 percent in controlled storage and it dropped 6 percent in Moghan conditions storage. While similar condition led to a decrease of 18 and 28 percent in Moghan seeds produced. When seeds were stored in controlled storage, indicated 17 percent higher protein content compared to storage in Moghan conditions. Catalase activity of Karaj seeds was higher than Moghan seeds (14%). Seed treatment had significant effect on peroxidase and MDA content, as peroxidase activity in treated seeds was 11 percent higher and MDA content was 8 percent lower than untreated seeds. Karaj seeds in controlled storage had the highest peroxidase activity and the lowest MDA content. So production seed conditions in Moghan was more critical in terms of temperature and humidity than Karaj so the biochemical properties of seeds have been affected. So that Moghan seeds had less storability after storing. On the other hand, seeds in Moghan store subjected to more critical temperature and humidity than controlled condition so more deteriorated. | ||
| کلیدواژه‌ها [English] | ||
| corn, enzymatic changes, germination, seed deterioration | ||
| مراجع | ||
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