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نقش باکتری‌های محرک رشد گیاهی بر برخی صفات جوانه‌زنی و ظهور گیاهچه در آزمایشگاه، صفات مورفولوژیکی و میزان رنگدانه‌های یونجه اکوتیپ همدانی(Medicago sativa L.) در شرایط گلخانه | ||
| علوم و فناوری بذر ایران | ||
| مقاله 13، دوره 8، شماره 2 - شماره پیاپی 16، بهمن 1398، صفحه 173-187 اصل مقاله (691.42 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22034/ijsst.2019.122512.1214 | ||
| نویسندگان | ||
| مرضیه سلطانی الکویی1؛ علی عباسی سورکی* 2؛ محسن مبینی دهکردی3؛ شهرام کیانی4 | ||
| 1دانشکده کشاورزی دانشگاه شهرکرد | ||
| 2دانشکده کشاورزی، دانشگاه شهرکرد | ||
| 3دانشکده علوم پایه- دانشگاه شهرکرد | ||
| 4دانشکده کشاورزی - دانشگاه شهرکرد | ||
| چکیده | ||
| به منظور مقایسه تاثیر باکتری‌های محرک رشد بر جوانه‌زنی بذر یونجه همدانی آزمایشی در قالب طرح بلوک کامل تصادفی با 4 تکرار و 8 تیمار تلقیح باکتریایی (شامل عدم تلقیح، کاربرد باکتریAcinetrobacter calcoaceticus PTCC 1318،Bacillus megaterium PTCC 1250، Enterobacter aerogenes PTCC 1221 بصورت منفرد و کاربرد توام دوگانه و سه گانه آن‌ها) انجام گرفت. آزمایش دیگری در گلخانه بصورت فاکتوریل در قالب طرح کاملا تصادفی با 3 تکرار انجام شد. تیمارهای باکتریایی ذکر شده بعنوان عامل اول و تزریق و عدم تزریق باکتری به خاک گلدان‌ها بعنوان عامل دوم در نظر گرفته شدند. نتایج نشان داد کاربرد باکتری شاخص وزنی بنیه گیاهچه، درصد و سرعت جوانه‌زنی را افزایش داد اما بر دیگر صفات یونجه در آزمایشگاه اثری نداشت. تلقیح بذرها با باکتری در گلخانه اثرات مثبت بر رشد گیاه یونجه گذاشت و درصد و سرعت سبز شدن، وزن خشک گیاه، حجم و طول ریشه، میزان کلروفیل a، b و کلروفیل کل برگ را افزایش داد. تزریق باکتری پای گیاهچه نیز برخی صفات را تحت تاثیر قرار داد که می‌تواند به دلیل استقرار بهتر و جمعیت بیشتر باکتری در ریزوسفر باشد. در مجموع کاربرد منفرد باکتری نتایج بهتری نسبت به کاربرد دو و سه باکتری با هم داشت لذا به نظر می‌رسد اثرات ضدیت باکتری‌ها بر یکدیگر ممکن است اثرات مثبت آن‌ها را کاهش دهد. | ||
| کلیدواژه‌ها | ||
| آسینتروباکتر؛ باسیلوس؛ سرعت سبز شدن؛ کلروفیل؛ یونجه | ||
| عنوان مقاله [English] | ||
| Effect of plant growth promoting bacteria on Seed germination and seedling emergence of alfalfa (Medicago sativa L.) hamedani ecotype in the laboratory, morphological characteristics and pigment contents in greenhouse conditions | ||
| نویسندگان [English] | ||
| marzie soltanialikooyi1؛ ali abbasi sourki2؛ mohsen mobini dehkordy3؛ shahram kiani4 | ||
| 1, Faculty of Agriculture, the University of Shahrekord | ||
| 2, Faculty of Agriculture, the University of Shahrekord | ||
| 3Faculty of Basic Sciences, Shahrekord | ||
| 4Faculty of Agriculture, Shahrekord University | ||
| چکیده [English] | ||
| In order to comparison the effects of growth promoting bacteria on germination of hamadani alfalfa seeds, a randomized complete block design was conducted with 4 replications and 8 levels of bacterial inoculation (include non-inoculation, application of Acinetrobacter calcoaceticus PTCC 1318, Bacillus megaterium PTCC 1250, Enterobacter aerogenes PTCC 1221 lonely and their pairwise and triple combination of them(. Another CRD factorial experiment was also conducted with 3 replications in greenhouse conditions. Mentioned bacterial treatments were as the first factor and injection of bacteria into the soil of pots as the second factor. The results showed that application of bacteria increased the vigor index of seedling, percentage and germination rate, but did not affect other alfalfa traits in the laboratory. Inoculation of seeds with bacteria in the pots had positive effects on alfalfa growth, percentage and rate of emergence, dry weight, root volume and length, chlorophyll a, b and total chlorophyll content. Injection of bacteria to the soil also affected some traits, which could be due to better placement and/or a larger population of bacteria in the rhizosphere. Application of bacteria lonely had better results than their combinations. It may be due to antagonistic effects of bacteria on each other, which may reduce positive effects. | ||
| کلیدواژه‌ها [English] | ||
| Acinetrobacter, Bacillus, Emergence rate, Chlorophyll, Medicago | ||
| مراجع | ||
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