| تعداد نشریات | 285 |
| تعداد نشریات سازمان | 83 |
| تعداد شمارهها | 3,110 |
| تعداد مقالات | 34,666 |
| تعداد مشاهده مقاله | 49,828,331 |
| تعداد دریافت فایل اصل مقاله | 80,172,870 |
اثرات سطوح مختلف اسید آمینه والین بر عملکرد، خصوصیات لاشه، کیفیت گوشت و بیان ژن های IGF-1 و انسولین در جوجههای گوشتی | ||
| علوم دامی | ||
| مقاله 7، دوره 32، شماره 125، اسفند 1398، صفحه 89-108 اصل مقاله (1.5 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/asj.2019.123483.1778 | ||
| نویسندگان | ||
| سیدسعید صادق زاده1؛ محسن دانشیار* 2؛ پرویز فرهومند3؛ محمد رضا یزدیان4؛ سید محمد هاشمی5 | ||
| 1دانشجوی دکتری گروه علوم دامی دانشکده کشاورزی دانشگاه ارومیه | ||
| 2دانشیار گروه علوم دامی دانشگاه ارومیه | ||
| 3استاد گروه علوم دامی دانشگاه ارومیه | ||
| 4استادیار گروه علوم پزشکی دانشگاه آزاد اسلامی واحد قم | ||
| 5استادیار گروه علوم دامی مرکز تحقیقات و کشاورزی استان قم | ||
| چکیده | ||
| این آزمایش به منظور بررسی اثرات سطوح متفاوت والین بر عملکرد، خصوصیات و کیفیت لاشه و بیان ژنهای فاکتور رشد شبه انسولین (IGF-1) و انسولین در جوجههای گوشتی انجام گرفت. آزمایش با 250 قطعه جوجه گوشتی نر یکروزه سویه راس 308 در قالب طرح کاملاً تصادفی، در 5 تیمار و 5 تکرار (در هر تکرار 10 پرنده) با 5 سطح ال-والین (100، 105، 110، 115 و 120 درصد نیازهای سویه راس) انجام شد. در 42 روزگی از هر تکرار یک قطعه جوجه جهت بررسی خصوصیات لاشه، کیفیت لاشه، IGF-1 سرم و بیان ژنهای IGF-1 و انسولین انتخاب شدند. بهبود افزایش وزن بدن و کاهش ضریب تبدیل با مصرف سطح 110 درصد والین در تمام دورههای آغازین، رشد و پایانی مشاهده گردید. مصرف 110 درصد والین موجب افزایش درصد لاشه، سینه، ران و کاهش چربی محوطه بطنی در سن 42 روزگی گردید. بیان ژنهای IGF-1و انسولین در عضله سینه و بافت کبدی در 42 روزگی با مصرف 115 درصد والین افزایش نشان داد. سطح 110 درصد والین موجب بهبود پروتئین، ماده خشک، چربی و خاکستر گوشت گردید. مصرف سطح 115 درصد والین در 42 روزگی موجب افزایش غلظتIGF-1 سرم شد. به طور کلی، مصرف 10 درصد ال-والین بالاتر از نیازهای سویه راس از راه فعال کردن mTOR در نتیجه فسفوریله شدن S6k1 منجر به آغاز ترجمه mRNA و سنتز پروتئین و در نتیجه موجب افزایش بیان ژنهای IGF-1 و انسولین، عملکرد و کیفیت لاشه و کاهش درصد چربی محوطه بطنی در جوجههای گوشتی نر سویه راس 308 میشود. | ||
| کلیدواژهها | ||
| انسولین؛ بیان ژن؛ جوجههای گوشتی؛ والین؛ IGF-1 | ||
| عنوان مقاله [English] | ||
| Effects of different levels of valine amino acid on performance, carcas traits, meat quality and insulin like growth factor-1 and insulin genes expression in male Ross 308 broiler chickens | ||
| نویسندگان [English] | ||
| saeid sadeghzadeh1؛ M. Daneshyar2؛ Parviz Farhoomand3؛ M. R. Yazdian4؛ S. M. Hashemi5 | ||
| 1PhD Student, Department of Animal Science, Faculty of Agriculture, University of Urmia I r a n | ||
| 3Department of Animal Science, Urmia University | ||
| چکیده [English] | ||
| The aim of this experimentwas to investiagatethe effects of different levels of valine on performance, carcass traits and quality and insulin like growth factor-1(IGF-1) and insulin genes expression in male Ross 308 broiler ckickens. Present study performed using 250 one day male broiler chicks Ross 308 in completiy randomized design including 25 pen, 5 treatments and 5 replicates each (10 chicks in each replicate) with 5 levels of valine (100, 105, 110, 115, 120 percent of Ross starin requirments). At day 42 of age, one chick from each replicate slaughtered to determine the carcass traits, meat quality, serum IGF-1 and insulin and IGF-1 genes expressions. The improved body weight gain and decreased feed conversion ratio during starter, grower and finisher periods were observed by consumption of 110% valine level. Consumption of 110% valine caused the increased carcass, breast and thigh and lowered abdominal fat at 42 days of age. The IGF-1 and insulin genes expression of breast muscle and liver increased by 115% valine. At day 42 of age, consumption of 110% valine improved the protein, dry matter, crude fat and ash content of meat. In conclusion, consumption of 10% higher than Ross valine requirements increases the IGF-I and Insuline genes expressions through up-reguletion of mTOR and promotion of S6k1 phosphorylation signaling mRNA translettion and protein synthesis and hence improves the performance and carcass quality and decreases the abdominal fat in Ross 308 broiler chickens. | ||
| کلیدواژهها [English] | ||
| Broiler Chicks, Gene Expression, IGF-1, Insulin, Valine | ||
| مراجع | ||
|
AOAC. (2000). Official Methods of Analysis AOAC Int. 17th ed., Arlington, VA. Baker, D.H. (2005). Tolerance for branched-chain amino acids in experimental animals and humans. The Journal of nutrition 135, 1585S-1590S. Block, K. P. and Harper, A. E. (1984). Valine metabolism in vivo:effects of high dietary levels of leucine and isoleucine. Metab-olism. 33:559–566. Butler, A.A., LeRoith, D., Minireview. (2001). tissue-specific versus generalized gene targeting of the igf1 and igf1r genes and their roles in insulin-like growth factor. physiology. Endocrinology.142:1685-1688. Corzo, A., Dozier III, W., Loar, R., Kidd, M. and Tillman, P. (2010). Dietary limitation of isoleucine and valine in diets based on maize, soybean meal, and meat and bone meal for broiler chickens. British poultry science 51, 558-563. Corzo, A., Dozier, W. A., Kidd, M. T., Mejia, L., Zumwalt, C.D., and Tillman, P. B. (2011). Nutritional feasibility of l-valine inclusion in commercial broiler diets. Poult. Sci. 20 :284–290. Corzo, A., Kidd, M., Dozier III, W. and Vieira, S. (2007). Marginality and needs of dietary valine for broilers fed certain all-vegetable diets. Journal of Applied Poultry Research 16, 546-554. Corzo, A., Loar, R.E., and Kidd, M.T. (2009). Limitations of isoleucine and valine in broiler chick diets. Poult. Sci. 88:1934-1938. Corzo, A., Moran Jr, E. and Hoehler, D. (2004). Valine needs of male broilers from 42 to 56 days of age. Poultry science, 83(6): 946-951. Cota, D., Proulx, K., Smith, K.A., Kozma, S.C., Thomas, G., Woods, S.C., Seeley, R.J.(2006). Hypothalamic mTOR signaling regulates food intake. Science. 312:927–930. D’Mello, J.P.F., and lewis, D. (1970). Amino acid interactions in chick nutrition. 2. Interrelationships between leucine, isoleucine and valine. Br. Poult. Sci. 11: 313-323. Duclos, M.J., Chevalier. B., Le Marchand-Brustel, Y., Tanti, J.F., Goddard, C. and Simon, J. (1993). Insulin-like growth factor-I-stimulated glucose transport in myotubes derived from chicken muscle satellite cells. J .Endocrinol.137:465-72. Everaert, N., Swennen, Q., Coustard, S.M., Willemsen, H., Careghi, C., Buyse, J., Bruggeman, V., Decuypere, E. and Tesseraud, S. (2010). The effect of the protein level in a pre-starter diet on the post-hatch performance and activation of ribosomal protein S6 kinase in muscle of neonatal broilers. British Journal of Nutrition 103, 206-211. Farran, M. and Thomas, O. (1992). Valine deficiency. 1. The effect of feeding a valine-deficient diet during the starter period on performance and feather structure of male broiler chicks. Poultry science, 71(11): 1879-1884. Fernandez, S.R., Aoyagi, S., Han, Y., Parsons, C.M. and Baker, D.H. (1994). Limiting order of amino acids in corn and soybean meal for growth of the chick. Poultry Science, 73(12): 1887-1896. Gasparino, E.O.A., Liveira Neto, R., Del Vesco1T, A.P., 1TPires, A.V., Batista, E., Voltolini, DM. and Souza krs, T. (2012). 1T Expression of growth genes in response to glycerol use in Japanese quail diets. 0TGenetics and Molecular Research 11 (3): 3063-3068. Harper, A., Miller, R. and Block, K. (1984). Branched-chain amino acid metabolism. Annual review of nutrition 4, 409-454. Kadlec, J., Hosnedlova, B., Rehout,V., Citek, J. and Vecerek, L.(2011). Insulin-like growth factor-I gene polymorphism and its association with growth and slaughter characteristics in broiler chickens. Journal of Agrobiology, 28(2),157-163. Kainulainen, H., Hulmi, J.J. and Kujala, U.M. (2013). Potential role of branched-chain amino acid catabolism in regulating fat oxidation. Exercise and sport sciences reviews, 41(4): 194-200. Kenneth, J., Livak., D and Thomas, D. (2001). Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 22DDCT Method. METHODS 25, 402–408. Kimball, S.R., and Jefferson, L.S. (2006). signaling pathways and molecular mechanisms through which branched-chain amino acids mediate translational control of protein synthesis. J. Nutr. 136:227-231. Lei, M., Nie, Q., Peng, X., Zhang, D. and Zhang, X. (2005). Single nucleotide polymorphisms of the chicken insulin-like factor binding protein 2 gene associated with chicken growth and carcass traits. Poultry science 84, 1191-1198. Lynch CJ, Hutson SM J, Patson B, Vaval A, Vary TC (2002a) Tissue specific effects of chronic dietary leucine and norleucine supplementation on protein synthesis in rats. Am J Physiol Endocrinol Metab 283:E824–E835. McMurtry, JP., Francis, GL. and Upton, Z. (1997). Insulin-Like Growth Factors in Poultry. Domestic Animal Endocrinology 14(4): 199-229. Meijer, A.J. and Dubbelhuis, P.F. (2004). Amino acid signalling and the integration of metabolism. Biochemical and biophysical research communications 313, 397-403. Naranjo, WM., Yakar, S., Sanchez-Gomez, M., Perez, AU. and Setser, J. (2002). Protein calorie restriction affects nonhepatic IGF-I production and the lymphoid system: studies using the liver-specific IGF-I gene-deleted mouse model. Endocrinology;143:2233-2241. Oudin, A., Chevalier, B., Simon, J. and Duclos, M.J. (1998). Muscle insulin-like growth factor-I (IGF-I) receptors in chickens with high or low body weight: effects of age and muscle fibre type. Growth Horm IGF .Res.8:243-50. Penz, J.r., Clifford, A., Rogers, A. and Kratzer, F. (1984). Failure of dietary leucine to influence the tryptophan-niacin pathway in the chicken. The Journal of nutrition, 114(1): 33-41. Rogero, M. M., and Tirapegui, J. (2008). Aspectos atuais sobre aminoácidos de cadeia ramificada e exercício físico. Rev. Bras. Cien. Farm., v.44, p.536-575. Rosselot, G., McMurtry, J.P., Vasilatos-Younken, R. and Czerwinski, S. (1995). Effect of exogenous chicken growth hormone (cGH) administration on insulin-like growth factor-I (IGF-I) gene expression in domestic fowl. Mol Cell Endocrinol;114:157-66. Rossi, L. and Tirapegui, J. (2005). Aminoácidos de cadeia ramificada e atividade física. Tirapegui, J. Nutrição, metabolismo e suplementação na atividade física. São Paulo: Editora Atheneu, 153-161. Selvarasu, K., Amutha, R., Edwin, S., Natarajan, A. and Mani, K. (2016). Dietary Valine Supplementation on Meat Quality Characteristics of Broilers. Shimomura, Y., Yamamoto, Y., Bajotto, G., Sato, J., Murakami, T. and Shimomura, N. (2006). Nutraceutical Effects of Branched-Chain Amino Acids on Skeletal Muscle. Journal of Nutrition. 529s-532s. Tremblay, F., Lavigne, C., Jacques, H. and Marette, A. (2007). Role of dietary proteins and amino acids in the pathogenesis of insulin resistance. Annu. Rev. Nutr. 27, 293-310. Tuttle, W.L. and Balloun, S.L. (1976). Leucine, isoleucine and valine interactions in turkey poults. Poultry science 55: 1737-1743. Van Vught, A.J., Nieuwenhuizen, A.G., Brummer, R.J., and Westerterp-Plantenga, M.S. (2008). Effects of oral ingestion of amino acids and proteins on the somatotropic axis. J. Clin. Endocrinol. Metab. 93: 584-590. Velloso, C.P. (2008). Regulation of muscle mass by growth hormone and IGF-I. British Journal of Pharmacology. 154: 557–56. Wu, G., Bazer, F., Burghardt, R., Johnson, G., Kim, S., Li, X., Satterfield, M. and Spencer, T. (2010). Impacts of amino acid nutrition on pregnancy outcome in pigs: mechanisms and implications for swine production. Journal of animal science 88, E195-E204. Xiayu, R., Xuelin, H., Zhicheng, Z. and Xin, L. (2013). An improvement of the 2ˆ(–delta delta CT) method for quantitative real-time polymerase chain reaction data analysis. Biostat Bioinforma Biomath. 3(3): 71–85. Yin, Y., Yao, K., Liu, Z., Gong, M., Ruan, Z., Deng, D., Tan, B., Liu, Z. and Wu, G. (2010a). Supplementing L-leucine to a low-protein diet increases tissue protein synthesis in weanling pigs Amino Acids .39 1477–1486. Zhang, S., Qiao, S., Ren, M., Zeng, X., Ma, X., Wu, Z., Thacker, P. and Wu, G. (2013). "Supplementation with branched-chain amino acids to a low-protein diet regulates intestinal expression of amino acid and peptide transporters in weanling pigs." Amino acids 45(5): 1191-1205. Zhang, Y., Guo, K., LeBlanc, R.E., Loh, D., Schwartz, G.J. and Yu, Y.H. (2007). Increasing dietary leucine intake reduces diet-induced obesity and improves glucose and cholesterol metabolism in mice via multimechanisms. Diabetes 56:1647–1654. Zhou, H., Mitchell, A., McMurtry, J., Ashwell, C. and Lamont, S.J. (2005). Insulin-like growth factor-I gene polymorphism associations with growth, body composition, skeleton integrity, and metabolic traits in chickens. Poultry science 84, 212-219. Zhou, H., Mitchell, A.D., McMurtry, J.P., Ashwell, C.M. and Lamont, S.J. (2005). Insulin-like growth factor-I gene polymorphism associations with growth, body composition, skeleton integrity, and metabolic traits in chickens. Poultry .Science.84,212-219. | ||
|
آمار تعداد مشاهده مقاله: 963 تعداد دریافت فایل اصل مقاله: 564 |
||