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بررسی شاخص های مهم مرتبط با بیماری ویروسی وای سیب زمینی (Potato virus Y)در مزارع توتون استان گلستان | ||
| آفات و بیماری های گیاهی | ||
| مقاله 7، دوره 87، شماره 2 - شماره پیاپی 109، اسفند 1398، صفحه 227-240 اصل مقاله (947.48 K) | ||
| نوع مقاله: بیماریشناسی گیاهی | ||
| شناسه دیجیتال (DOI): 10.22092/jaep.2019.127197.1297 | ||
| نویسندگان | ||
| پریسا شریفی نظام آباد1؛ سعید نصراله نژاد* 2؛ محمدعلی آقاجانی3؛ اکبر دیزجی4؛ احمد ندیمی5 | ||
| 1گروه گیاه پزشکی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان | ||
| 2دانشیار گروه گیاهپزشکی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان ایران | ||
| 3بخش تحقیقات گیاه پزشکی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان گلستان، گرگان. | ||
| 4عضو هیات علمی گروه گیاهپزشکی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران | ||
| 5گروه گیاهپزشکی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران؛ | ||
| چکیده | ||
| ویروس وای سیبزمینی (Potato virus Y, PVY)، گونۀ تیپ جنس Potyvirus از تیرة Potyviridae، از جمله ویروسهای مخرب توتون در دنیا میباشد. به منظور بررسی وضعیت بیماری ناشی از این ویروس در مزارع توتون استان گلستان، طی فصل زراعی 1396، تعداد 15 مزرعه توتون در سه شهرستان گرگان، علیآباد و مینودشت انتخاب شده و در فواصل زمانی 15 روزه مورد بازدید قرار گرفت. در هر مزرعه 50 بوته انتخاب و شاخصهای میزان وقوع بیماری (DI) و فراوانی دو شته ناقل سبز هلو (Myzus persicae, MP) و قرمز توتون (Myzus persicae ssp. nicotiana, MPN) در آنها ارزیابی گردید. به منظور ردیابی و ارزیابی غلظت نسبی ویروس (OD) از روش DAS-ELISA با استفاده از آنتیسرم چند همسانهای اختصاصی PVY استفاده شد. درصد آلودگی PVY در بین ارقام مختلف توتون متفاوت بوده و بیشینه و کمینه درصد آلودگی به تریتب در ارقام PVH03 (6/33 %) و بارلی (2/6 %) ارزیابی شد. بر اساس نتایج حاصل از تحلیل آماری دادهها، بین ارقام توتون در تمام شاخصهای مورد ارزیابی تفاوت معنیدار بوده و ارقام باسما و بارلی نسبت به ارقام گرمخانهای (PVH03، NC100 و K326) دارای حساسیت کمتری به PVY بودند. بیشترین جمعیت هر دو شته روی رقم K326 مشاهده گردید. برای شاخص-های DI و OD ارتباط همبستگی با دما غیرمستقیم و رطوبت نسبی و میزان بارندگی مستقیم ارزیابی شد و DI بیشترین میزان همبستگی را با تغییرات بارندگی (r= 0.98 و P= 0.004) داشت. | ||
| کلیدواژهها | ||
| توتون؛ شته سبز هلو؛ PVH03؛ Disease incidence؛ DAS-ELISA | ||
| عنوان مقاله [English] | ||
| Investigating the Potato virus Y main disease indicators in tobacco fields of Golestan province | ||
| نویسندگان [English] | ||
| parisa sharifi-nezamabad1؛ saeed nasrollanejad2؛ Mohammad Ali Aghajani3؛ Akbar Dizadji4؛ Ahmad Nadimi5 | ||
| 1department of plant pathology, faculty of plant production, Gorgan university of Agricultural sciences and Natural resource | ||
| 2Associate Prof, Dept of Plant Protection, Gorgan University of Agricultural Sciences and Natural Resources | ||
| 3Department of Plant Protection, Agricultural and Natural Resources Research Center of Golestan province, Gorgan. | ||
| 4Department of Plant Protection, college of Agriculture and Natural Resources, University of Tehran | ||
| 5Department of Plant Protection, Faculty of Plant Production, University of Agricultural Sciences and Natural Resources, Gorgan, Iran | ||
| چکیده [English] | ||
| Potato virus Y, is the type member of the genus Potyvirus, family Potyviridae is of the most destructive viruses of tobacco worldwide. In order to detect PVY and its distribution in Golestan province, several surveys were conducted during July to October 2017 at 15 days intervals in tobacco fields of Gorgan, Aliabad and Minoodasht. 50 plant was selected for each farm and various disease indicators and the virus vector (MP and MPN( were evaluated. Double antibody sandwich-ELISA (DAS-ELISA) using PVY-specific polyclonal antibodies was used to estimate changes in the relative concentrations of PVY. The rate of PVY infection was different among tobacco cultivars and the maximum and minimum infection rates were belonged to PVH03 (33.6%) and Barley (6.2%) cultivars respectively. Based on the statistical analysis of data, there was a significant difference for disease indicators and the virus vector on different tobacco cultivars, as Basma and Barley were less susceptible to PVY than Flue-cured cultivars (PVH03, NC100 and K326). Also, the K326 cultivar has the highest Myzus persicae ssp. nicotiana and Myzus persicae populations. There was a direct correlation between DI and OD and relative humidity and rainfall changes so that DI with rainfall changes had the highest Correlation (r = 0.98 and P = 0.004). | ||
| کلیدواژهها [English] | ||
| Tobacco, Disease incidence, DAS-ELISA, Myzus persicae, PVH03 | ||
| مراجع | ||
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