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Evaluation of Apoptosis Induction by Newcastle Disease Virus LaSota Strain in Human Breast Carcinoma Cells | ||
| Archives of Razi Institute | ||
| مقاله 8، دوره 75، شماره 3، آذر 2020، صفحه 367-376 اصل مقاله (393.52 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2019.125824.1322 | ||
| نویسندگان | ||
| A. Kalantari1؛ S. Farashi Bonab* 2؛ H. Keyvanfar3؛ P. Mortazavi4 | ||
| 1Department of Microbiology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 2Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran | ||
| 3Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran | ||
| 4Department of Pathology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| چکیده | ||
| The innovation of therapeutic modalities with better clinical efficacy is necessary for the treatment of patients with advanced cancers. Newcastle disease virus (NDV), an avian pathogenic virus, is one of the most promising oncolytic viruses that can replicate selectively in human cancer cells. In humans, NDV can cause transient conjunctivitis and mild flu-like symptoms. However, this virus poses no hazard to human health. The elucidation of the mechanisms of cancer cell killing by NDV is helpful for the clinical application of NDV in cancer patients. Regarding this, the present study was performed to evaluate apoptosis induction by NDV LaSota strain vaccine in human breast carcinoma cells. To this end, MCF-7 cells, a human breast adenocarcinoma cell line, were infected with NDV in vitro. Tumor cell cytotoxicity, apoptosis induction, and expression levels of apoptosis-related genes were examined in NDV-infected breast carcinoma cells. Tumor cell cytotoxicity was measured by 3-[4,5-dimethylthiazol-2yl]2,5-diphenyl-tetrazoliumbromide (MTT) assay. The induction of apoptosis was assessed by annexin V/propidium iodide staining. In addition, the expression levels of apoptosis-related genes were evaluated using real-time reverse transcription polymerase chain reaction (PCR) technique. The NDV showed cytotoxic effects on MCF-7 cells and induced apoptosis in the infected carcinoma cells. The gene expression levels of BAX, caspase-9, and caspase-3, but not BAK-1, were increased in NDV-infected cancer cells, compared to the gene expression levels in the non-infected cancer cells. These results suggest that the induction of the intrinsic pathway of apoptosis is one of the mechanisms that can contribute to cancer cell killing by NDV. Additional studies are required to investigate other probable mechanisms involved in NDV-mediated cancer cell killing. | ||
| کلیدواژه‌ها | ||
| Cancer؛ Newcastle disease virus؛ Oncolytic virus؛ Apoptosis؛ Breast adenocarcinoma cell | ||
| عنوان مقاله [English] | ||
| ارزیابی آپوپتوز القاء شده توسط ویروس بیماری نیوکاسل سویه لاسوتا در سلولهای سرطانی پستان انسان | ||
| چکیده [English] | ||
| ابداع روش های درمانی با کارایی بهتر برای درمان بیماران مبتلا به سرطانهای پیشرفته ضروری میباشد. ویروس بیماری نیوکاسل (Newcastle disease virus/NDV) که یک ویروس بیماریزای پرندگان میباشد از جمله ویروسهای انکولیتیک میباشد که میتواند به صورت انتخابی در سلولهای سرطانی انسان تکثیر یابد.NDVدر انسان التهاب ملتحمه موقتی و علایم شبیه به آنفلوانزای خفیف ایجاد می کند. با وجود این، NDV خطری برای سلامتی انسان ایجاد نمیکند. پی بردن به مکانیسمهایی که توسط آنهاNDVسلولهای سرطانی را نابود میکند به کاربرد بالینیNDVدر درمان بیماران سرطانی کمک میکند. هدف از مطالعه حاضر ارزیابی آپوپتوز القاء شده در سلولهای سرطانی پستان انسان توسطNDVبود. سلولهای 7MCF- (رده سلولی سرطان پستان انسان) باNDVدر محیط کشت آلوده شدند. سیتوتوکسی سیتی سلولهای توموری، القاء آپوپتوز و سطح بیان ژنهای مرتبط با آپوپتوز در سلولهای توموری آلوده بهNDV اندازه گیری شد. سیتوتوکسی سیتی ویروس برای سلولهای سرطانی با روش MTT (3- (4،۵-دی متیل 2-تیازول) 2، 5-دی فنیل تترازولیوم بروماید) ارزیابی شد. القاء آپوپتوز با رنگ آمیزی انکسین V/پروپیدیوم یدید و بیان ژنهای مرتبط با آپوپتوز باReal-time RT-PCR ارزیابی شد.NDVنسبت به سلولهای سرطانی پستان اثرات سیتوتوکسیک نشان داد و در سلولهای سرطانی باعث القاء آپوپتوز شد. سطح بیان ژنهای BAX، کاسپاز-9 و کاسپاز-3 در سلولهای سرطانی آلوده بهNDVافزایش یافت ولی بیان ژن ۱-BAK افزایش پیدا نکرد. این نتایج نشان میدهد القاء مسیر داخلی آپوپتوز میتواند یکی از مکانیسمهای تخریب سرطان توسطNDVباشد. مطالعات بیشتری برای بررسی سایر مکانیسمهای احتمالی تخریب سلول سرطانی توسطNDVلازم است. | ||
| کلیدواژه‌ها [English] | ||
| سرطان, ویروس بیماری نیوکاسل, ویروس انکولیتیک, آپوپتوز, سلول سرطانی پستان | ||
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| مراجع | ||
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