Ebrahimi, M., Grigorian, S., Fallah Mehrabadi, M., Shoushtari, A. (2021). Serological survey of H9N2 influenza viruses in rural chicken of Northern provinces, Iran. , 34(1), 15-25. doi: 10.22092/vj.2019.127568.1613
M. Ebrahimi; S. Grigorian; M. H. Fallah Mehrabadi; A. Shoushtari. "Serological survey of H9N2 influenza viruses in rural chicken of Northern provinces, Iran". , 34, 1, 2021, 15-25. doi: 10.22092/vj.2019.127568.1613
Ebrahimi, M., Grigorian, S., Fallah Mehrabadi, M., Shoushtari, A. (2021). 'Serological survey of H9N2 influenza viruses in rural chicken of Northern provinces, Iran', , 34(1), pp. 15-25. doi: 10.22092/vj.2019.127568.1613
Ebrahimi, M., Grigorian, S., Fallah Mehrabadi, M., Shoushtari, A. Serological survey of H9N2 influenza viruses in rural chicken of Northern provinces, Iran. , 2021; 34(1): 15-25. doi: 10.22092/vj.2019.127568.1613

Serological survey of H9N2 influenza viruses in rural chicken of Northern provinces, Iran

تحقیقات دامپزشکی و فرآورده‌های بیولوژیک
Article 3, Volume 34, Issue 1, March 2021, Pages 15-25    PDF (1.71 M)
Document Type: Full Research Paper
DOI: 10.22092/vj.2019.127568.1613
Authors
M. Ebrahimi1; S. Grigorian2; M. H. Fallah Mehrabadi3; A. Shoushtari* 1
1Razi Vaccine and Serum Research Institute, Agricultural Research,Education and Extension Organization (AREEO), Tehran, Iran.
2Department of Virology & Microbiology, College of Veterinary Medicine, Armenian State University, Yerevan, Armenia.
3Razi Vaccine and Serum Research Institute, Agricultural Research,Education and Extension Organization (AREEO), Tehran, Iran
Abstract
Avian Influenza is an acute and contagious disease of humans and animals, an agent of Orthomyxoviridae family. H9N2 subtype of avian influenza virus (AIVs) is one of the main problems of poultry industry in Iran. The aim of study was to investigate the status of domestic poultry contamination and identifying the hemagglutinin gene of H9N2 isolates in northern providences of Iran.This cross-sectional study was carried out in rural population of Gilan, Mazandaran and Golestan provinces from 1393 to 1394.The sera and cloacal swabs were sampled and tested, using a haemagglutination inhibition test and reverse transcriptase polymerase chain reaction respectively. The RNA was extracted, HA and NA genes was proliferated, using; two pairs of full-open-reading frame universal primer pairs, Titan one step tube RT-PCR system (Roche). A total of 70 villages and 993 birds; (Mazandaran province, 20 villages with 276 birds , Gilan province, 20 villages, with 347 birds and Golestan province, 30 villages with , 370 birds) were sampled. The sera sample of 67 villages (96%) out of 70 and 557 birds (56%) out of 993 were positive. The only H9N2 subtype was isolated of swab sample named A / chicken / Iran / RZ18 / 2014.Both HA and NA genes were common close to Iran / Markazi / 2014 and Iran / MMV9- 2013 isolate. The results reveal a high level of circulation of H9N2 influenza viruses in domestic poultry in the northern provinces of Iran.
Keywords
Influenza; H9N2; Northern provinces; backyard poultry; Sero-servey
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