Dirandeh, E., Ansari-pirsaraei, Z. (2021). The relationship between heat shock proteins and pregnancy loss in Holstein dairy cows. , 34(2), 109-104. doi: 10.22092/vj.2020.341931.1692
E. Dirandeh; Z. Ansari-pirsaraei. "The relationship between heat shock proteins and pregnancy loss in Holstein dairy cows". , 34, 2, 2021, 109-104. doi: 10.22092/vj.2020.341931.1692
Dirandeh, E., Ansari-pirsaraei, Z. (2021). 'The relationship between heat shock proteins and pregnancy loss in Holstein dairy cows', , 34(2), pp. 109-104. doi: 10.22092/vj.2020.341931.1692
Dirandeh, E., Ansari-pirsaraei, Z. The relationship between heat shock proteins and pregnancy loss in Holstein dairy cows. , 2021; 34(2): 109-104. doi: 10.22092/vj.2020.341931.1692

The relationship between heat shock proteins and pregnancy loss in Holstein dairy cows

تحقیقات دامپزشکی و فرآورده‌های بیولوژیک
Article 12, Volume 34, Issue 2, June 2021, Pages 109-104    PDF (543.68 K)
Document Type: Full Research Paper
DOI: 10.22092/vj.2020.341931.1692
Authors
E. Dirandeh* ; Z. Ansari-pirsaraei
Department of Animal Science, Sari Agricultural Sciences and Natural Resources University, Sari, Mazandaran, Iran
Abstract
The objective of this was to consider relationship between heat shock proteins (HSP) with early embryo loss and pregnancy loss in Holstein dairy cows during summer season. Thirsty-two Holstein cows (with milk production> 30 Kg/d and 3.3±1.7 in parity) were selected. All cows were synchronized by G7G heat synchronization protocol. Blood samples were collected from all cows at d 16 after timed artificial insemination (TAI) to measure ISG15 gene expression. According to ISG15 results, 16 cows showed early embryo loss, then HSP gene family (HSP27, HSP40, HSP60, HSP70 and HSP 90) were compared between healthy cows and those with early embryo loss using t-test. Beta-actin gene was used as a housekeeping gene to compare results. Results showed ISG15 gene expression were greater in pregnant cows compared to early embryo loss (6.6 fold). Among HSP family gene considered in present study, HSP27 and HSP60 gene expression were similar between groups (p < 0.05). HSP40, HSP70 and HSP 90 genes expression were lower in pregnant cows compared to early embryo loss (4.7, 3.6 and 5.2 fold, respectively). In conclusion present study results showed among HSP family, there was an association among HSP40, HSP70 and HSP 90 genes expression and early embryo loss.
Keywords
Gene expression; heat shock protein; dairy cow; early embryo loss
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