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Biological investigation of Lilium ledebourii in Iran | ||
| Rostaniha | ||
| دوره 21، شماره 2 - شماره پیاپی 60، اسفند 2020، صفحه 141-153 اصل مقاله (559.7 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22092/botany.2020.341949.1190 | ||
| نویسندگان | ||
| Salman Maleki Choreh1؛ Fatemeh Zarinkamar* 2؛ Mehrdad Nazari3 | ||
| 1MSc Student of Cellular and Developmental Biology, Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran | ||
| 2Associate Prof., Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran | ||
| 3PhD in Plant Physiology, Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran | ||
| چکیده | ||
| Lilium ledebourii is a rare Asian species and is an endemic plant of Iran which grows in Gilan province in Damash highlands. In this study, the structural features of flower including the study of sections such as carpel, stamen, and tepals were performed. This plant had a full flower that consisted of six tepals (three petals and three petal-like sepals), six free stamens and a 3-lobed gynoecium. In the cross-section of tepals, the stomata as well as cells containing anthocyanins were observed. In stamens, anther (epidermis, endothecium, middle layers and an innermost tapetum), and filament were observed. In the gynoecium, ovary (ovary wall, locule, funiculus, anatropous ovule, different arrangements of vascular bundles, transmitting tissue and stomata), style (stylar canal and transmitting tissue), and dry stigma with elongated papillae tissue were observed. Besides, the presence of alkaloids in the bulb was tested using Wagner and Dragendorff reagents and the total alkaloid content in three types of methanol, ethanol, and butanol extraction was investigated. The ethanol extract had a significant difference with other extractions. The methanol extract was chosen for further investigation by gas chromatography-mass spectrometry (GC-MS) and two compounds of Pterin-6-carboxylic acid, and Pyrrolizin-1,7-dione-6-carboxylic acid, methyl (ester) were detected. | ||
| کلیدواژهها | ||
| Alkaloid؛ anatropous ovule؛ Liliaceae؛ papillae؛ petal-like sepal؛ tepal | ||
| عنوان مقاله [English] | ||
| بررسی زیستشناسی سوسن چلچراغ در ایران | ||
| نویسندگان [English] | ||
| سلمان ملکی چره1؛ فاطمه زرین کمر2؛ مهرداد نظری3 | ||
| 1دانشجوی کارشناسی ارشد زیستشناسی سلولی و تکوینی، گروه علوم گیاهی، دانشکده علوم زیستی، دانشگاه تربیت مدرس، تهران، ایران | ||
| 2دانشیار گروه علوم گیاهی، دانشکده علوم زیستی، دانشگاه تربیت مدرس، تهران، ایران | ||
| 3دکترای فیزیولوژی گیاهی، گروه علوم گیاهی، دانشکده علوم زیستی، دانشگاه تربیت مدرس، تهران، ایران | ||
| چکیده [English] | ||
| سوسن چلچراغ (Lilium ledebourii) یک گونه نادر آسیایی از تیره سوسنیان است که بومی ایران بوده و در استان گیلان، در ارتفاعات منطقه داماش رشد میکند. در این مطالعه، ویژگیهای ساختاری گل مشتمل بر مطالعه بخشهایی از قبیل برچه، پرچم و گلپارها انجام شد. این گیاه دارای یک گل کامل متشکل از شش گلپار (سه گلبرگ و سه کاسبرگ گلبرگآسا)، شش پرچم آزاد و تخمدان سه برچهای است. در سطح مقطع گلپارها، روزنهها و همچنین سلولهای حاوی آنتوسیانینها مشاهده شدند. در پرچمها، بساک (اپیدرم، لایه مکانیکی لایههای میانی و سلولهای لایه پرستار میانی و میله مشاهده شد. در مادگی، تخمدان (دیواره، حفره، بند تخمک، تخمک آناتروپ، گروههای مختلف دستههای آوندی، بافت انتقال و روزنهها)، خامه (کانال خامه و بافت انتقال دهنده) و کلاله خشک با بافت پاپیلای کشیده مشاهده شد. علاوه بر این، حضور آلکالوییدها در پیاز گل با استفاده از معرفهای واگنر و دراژندورف مورد آزمایش قرار گرفت و محتوای کل آلکالوییدها در سه نوع عصاره متانولی، اتانولی و بوتانولی بررسی شد. عصاره اتانولی با سایر عصارهها تفاوت معنیداری داشت. عصاره متانولی برای بررسی بیشتر با استفاده از کروماتوگرافی گازی-طیفسنجی جرمی (GC-MS) انتخاب شد و دو ترکیب Pterin-6-carboxylic acid و Pyrrolizin-1,7-dione-6-carboxylic acid, methyl (ester) شناسایی گردیدند. | ||
| کلیدواژهها [English] | ||
| آلکالویید, تخمک آناتروپ, پاپیله, سوسنیان, کاسبرگ شبیه گلبرگ, گلپار | ||
| مراجع | ||
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