Rahimizadeh, K., Hashemitabar, G., Basami, M., Haghparast, A., Sankian, M., Veedu, R. (2021). Development of novel oligonucleotides targeting erythrocyte type B markers for drug delivery applications. , 34(4), 45-53. doi: 10.22092/vj.2020.343428.1732
K Rahimizadeh; Gh. Hashemitabar; M Basami; A Haghparast; M Sankian; R. N. Veedu. "Development of novel oligonucleotides targeting erythrocyte type B markers for drug delivery applications". , 34, 4, 2021, 45-53. doi: 10.22092/vj.2020.343428.1732
Rahimizadeh, K., Hashemitabar, G., Basami, M., Haghparast, A., Sankian, M., Veedu, R. (2021). 'Development of novel oligonucleotides targeting erythrocyte type B markers for drug delivery applications', , 34(4), pp. 45-53. doi: 10.22092/vj.2020.343428.1732
Rahimizadeh, K., Hashemitabar, G., Basami, M., Haghparast, A., Sankian, M., Veedu, R. Development of novel oligonucleotides targeting erythrocyte type B markers for drug delivery applications. , 2021; 34(4): 45-53. doi: 10.22092/vj.2020.343428.1732

Development of novel oligonucleotides targeting erythrocyte type B markers for drug delivery applications

تحقیقات دامپزشکی و فرآورده‌های بیولوژیک
Article 6, Volume 34, Issue 4, January 2022, Pages 45-53    PDF (687.91 K)
Document Type: Full Research Paper
DOI: 10.22092/vj.2020.343428.1732
Authors
K Rahimizadeh1; Gh. Hashemitabar* 1; M Basami2; A Haghparast1; M Sankian3; R. N. Veedu4
1Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
2Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran
3Immunobiochemistry laboratory, Immunology Research Center, Bu-Ali Research Institute, Mashhad, Iran
4Center for Comparative Genomics, Murdoch University, Perth 6150, Australia
Abstract
Nucleic acid aptamers have been emerged as potential molecules for target-specific drug delivery because of their ability to bind targets with very high affinity and specificity. Rapid renal clearance is one of the major issues associated with the vascular delivery of nucleic acid-based drugs. To circumvent this problem, this study was designed to envision the development of nucleic acid aptamers specific to human erythrocyte as erythrocytes travel through the cardiovascular system. Erythrocytes have special properties which make them peculiar and specific as a physiological carrier for drug delivery For this purpose, we have performed Cell-SELEX procedure using human erythrocytes containing 4-O-((2-O-(β-D-fucopyranosyl)-3-O-(α-D-galactopyranosyl))-β-galactopyranosyl)-2-acetamido-2-deoxy-α-D-glucopyranoside on the surface as a model target. After completing 15 rounds of selection, sequencing of the product identified RNV543 and RNV544 as potential oligonucleotides specific to the target human erythrocyte. The binding affinity of both RNV543 and RNV544 was confirmed by flowcytometry. This study showed there is Aptamers which can have specific binding to erythrocyte type B.
Keywords
Functional nucleic acids; Cell-SELEX; oligonucleotides; erythrocyte; drug deliver
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