| تعداد نشریات | 284 |
| تعداد نشریات سازمان | 82 |
| تعداد شماره‌ها | 3,039 |
| تعداد مقالات | 34,000 |
| تعداد مشاهده مقاله | 46,061,150 |
| تعداد دریافت فایل اصل مقاله | 77,291,978 |
مطالعه اثر پیری تسریع شده و اسید جیبرلیک بر خصوصیات جوانه زنی، پر اکسیداسیون لیپیدها، پروتئین محلول، فنل کل و هدایت الکتریکی بذر گندم (رقم پیشتاز) (Triticum aestivum L.) | ||
| علوم و فناوری بذر ایران | ||
| مقاله 4، دوره 9، شماره 2 - شماره پیاپی 18، شهریور 1399، صفحه 49-60 اصل مقاله (1.26 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22034/ijsst.2020.115718.1136 | ||
| نویسندگان | ||
| زهرا محدث اردبیلی1؛ حسین عباسپور2؛ رضا توکل افشاری* 3؛ سید محسن نبوی کلات4 | ||
| 1گروه زیست شناسی، واحددامغان، دانشگاه آزاد اسلامی، دامغان، ایران | ||
| 2گروه زیست شناسی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران | ||
| 3استادگروه زراعت، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران | ||
| 4گروه زراعت ، ,واحدمشهد، دانشگاه ازاداسلامی، مشهد،ایران | ||
| چکیده | ||
| به‌منظور مطالعه اثر پیری تسریع شده و پرایمینگ بذر با اسید جیبرلیک بر خصوصیات جوانه‌زنی، پر اکسیداسیون لیپیدها، پروتئین محلول، فنل کل و هدایت الکتریکی بذر گندم (رقم پیشتاز) یک آزمایش فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار در آزمایشگاه تکنولوژی بذر گروه زراعت دانشکده کشاورزی دانشگاه فردوسی مشهد در سال 1395 انجام شد. عوامل آزمایش شامل پیری بذرتحت شرایط دمای 40 درجه سانتی گرادو 100% رطوبت در سه سطح [زوال کم (4 روز)، زوال متوسط (6 روز) و زوال زیاد (7 روز)] و پرایمینگ بذر با اسید جیبرلیک در 4 سطح (0، 25، 50 و 100 پی‌پی-ام) بود. نتایج تجزیه واریانس نشان داد که اثر پیری بذر، پرایمینگ با اسید جیبرلیک و اثر متقابل دو عامل بر تمامی صفات اندازه‌گیری شده معنی‌دار بود. بر اساس نتایج مقایسه میانگین صفات با افزایش مدت‌زمان پیری درصد و سرعت جوانه‌زنی، پروتئین محلول و فنل کل کاهش یافت؛ اما میزان مالون‌دی‌آلدئید و هدایت الکتریکی به علت آسیب وارده به غشای سلولی با افزایش سطوح پیری افزایش یافت. بررسی اثرات متقابل دو عامل نشان داد که پرایمینگ با اسید جیبرلیک اثرات منفی پیری بذر بر تمامی صفات اندازه‌گیری شده را کاهش می‌دهد. | ||
| کلیدواژه‌ها | ||
| جیبرلیک اسید؛ درصد جوانه‌زنی؛ زوال؛ سرعت جوانه‌زنی؛ مالون دی آلدئید | ||
| عنوان مقاله [English] | ||
| The study on effect of accelerated aging and gibberellic acid on germination characteristics, lipids peroxidation, soluble protein, total phenol and electrical conductivity of wheat seed (Pishtaz Cultivar) (Triticum aestivum L.) | ||
| نویسندگان [English] | ||
| zahra Mohaddes Ardebili1؛ Hossein Abbaspour2؛ Reza Tavakkol afshari3؛ Seid Mohsen Nabavi Kalat44 | ||
| 1Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran. | ||
| 2Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran | ||
| 3Department of Agrotechnology, Faculty of Agriculture, Ferdowsi University of Mashhad, P.O. Box 91779-48974, Mashhad, Iran | ||
| 4Department of Agronomy, Mashhad Branch, Islamic Azad University, Mashhad, Iran. | ||
| چکیده [English] | ||
| In order to study the effect of accelerated aging and priming with gibberellic acid on germination characteristics, lipids peroxidation, soluble protein, total phenol and electrical conductivity of wheat seed (Pishtaz Cultivar) an experiment in factorial laid out completely randomized design with three replications was conducted at Seed Technology Laboratory, Department of Agronomy, Faculty of Agriculture, Ferdowsi University, Mashhad, Iran, in 2016. Factors were included of seed aging(100% relative humidity at 40°C) in three levels [low deterioration (4 days), medium deterioration (6 days) and high deterioration (7 days)] and priming with gibberellic acid in four levels (0, 25, 50 and 100 ppm). Analysis of variance showed that the effect of seed aging, priming with gibberellic acid and interaction between two factors on all traits were significant. Based on the means comparison of the traits germination percentage, germination rate, soluble protein and total phenol decreased with increasing aging time. But, the level of malondyaldeid and electrical conductivity increased due to damage to cell membrane with increasing aging time. Investigation of interaction between two factors showed that the priming with gibberellic acid reduced the negative effects of seed aging on all traits. | ||
| کلیدواژه‌ها [English] | ||
| Deterioration, Gibberellic acid, Germination percentage, Germination speed, Malondyaldeid | ||
| مراجع | ||
|
Al-Maskri, A.Y., M.M. Khan, M. JavedIqbal, and M. Abbas. 2004. Germinability, vigour and electrical conductivity changes in accelerated aged watermelon (Citrullusl anatus L.) seeds. J. Food Agric. Environ. 3: 100-103. Avila, M.R., A.L. Braccini, C. Giatti, M. Souza, G. Mandarino, and G.L.Bazo, Y.C.F. Cabral. 2012. Physiological quality, content and activity of antioxidants in soybean seeds artificially aged.Revista Brasileira de Sementes. 34 (3): 397 – 407. Bailly, C. 2004. Active oxygen species and antioxidants in seed biology. Seed Sci. Res. 14:93-107 Bailly, C., A. Benamar, F. Corbineau, and D. Come. 2000. Antioxidant systems in sunflower (Helianthus annuus L.) seeds as affected by priming. Seed Sci. Res. 10:35–42. Black, M., and J.D. Bewley. 2009. Seed Technology and its Biological Basis. Translated by R. Tavakkol Afshari. A, Abbasi Surki. E, Ghasemi. University of Tehran Press. Bradford, M.N. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochem. 72:248-254. Chang, S.M., and J.M. Sung. 1998. Deteriorative changes in primed sweetcorn seeds during storage. Seed Sci Technol 26: 613–626. Dahal, P., K.J. Bradford, and R.A. Jones. 1990. Effects of priming and endosperm integrity on seed germination rates of tomato genotypes. J. Exp. Bot. 41: 1441–1453 Goel, A., and I.Sheoran. 2003. Lipid peroxidation and peroxide-scavenging enzymes in cotton seeds undernatural ageing. Biol. Plantarum 46: 429-434. Goel,A., A.K.Goel, and I. Sheoran. 2003. Changes in oxidative stress enzymes during artificial ageing in cotton (Gossypium hirsutum L.) seeds J. Plant Physiol. 160: 1093–1100. Grilli, I., E. Bacci, T. Lombardi, C. Spano, and C. Floris. 1995. Natural Aging: Poly (A) polymerase in germination embryos of Triticum durum wheat. Ann. Bot. 76: 15–21. Ghaderi-Far, F., A.Soltani, H. Sadeghipour. 2014. Changes in soluble carbohydrates and reactive oxygen species -scavenger enzymes activity in Medicinal Pumpkin during storage at different temperature and seed moisture. EJCP., 7 (1): 157-179. Ghahremani,S., M. Sedghi, and H. Tavakoli. 2014. Effect of gibberellin and salicylic acid on germination and enzyme activity Antioxidant in of aging cucurbit. J. Seed Res. 2:20-29. Hampton, J.G., and D.M. TeKrony. 1995. Handbook of Vigor Test Methods. The International Seed Testing Association, Zurich. Heath, R.L., and L. Packer. 1969. Photoperoxidation in isolated chloroplast, Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys. 125: 189-198. Hoseinikhah, F.S., S. Parsa, R. Tavakkol Afshari,M. Jami Al Ahmadi, A.Esmaeili. 2014. Effect of Salicylic Acid and Glycerol Acid on Prevention Processes Improve the decay of two sesame seeds(Sesamum indicum).Iranian Crop Sci. 5:425-431. (In Persian) Hu, D., M.a. Gang, Q. Wang, J. Yao, Y. Wang, H.W. Pritchard, and X.F. Wang. 2012. Spatial and temporal nature of reactive oxygen species production and programmed cell death in elm (Ulmus pumila L.) seeds during controlled deterioration. Plant Cell. Environ. 35: 2045-2059. Jeng, T.L., and J.M. Sung. 1994. Hydration effect on lipidperoxidation and peroxide-scavenging enzymes activity of artificially age peanut seed. Seed Sci.Technol. 22: 531-539. Justice, O.L., and L.N. Bass. 1979. Principles and practices of seed storage. Castele House publications, London. Kalpana, R., and K.V. Madhava Rao. 1994. Absence of the role of lipid peroxidation during accelerated ageing of seed of pigeon pea (Cajanus cajan (L.) Millsp.) Cultivars. Seed Science and Technology 22: 253-260. Klimezak, I., and M. Malecka. 2006. Effect of storage on the content of polyphenols, vitamin C and the antioxidant activity of orange juices. J. Food Composition and Analysis. 20: 313- 322. Karta, k.k., and A. Bekele. 2012. Influence of seed priming on germination and vigor traits of Vicia villosa. Afr. J. Agric. Res. 7 (21): 3202- 3208. Lehner, A., N. Mamadou., P. Poels., D. Come, C. Bailly, and F. Corbineau. 2008. Change in soluble carbohydrates, lipid peroxidation and antioxidant enzyme activityes in the embryo during aging in wheat grains. J. Cereal Sci. 47: 555-565. Lu, X.X., X.L. Chen, and Y.H. Guo. 2005. Seed germinability of 23 crop species after a decade of storage in the National Gene Bank of China. Agric. Sci. China 4: 408-412. McDonald, M.B. 1999. Seed deterioration: physiology, repair and assessment. Seed Science and Technology 27: 177–237. Mehravar, M., A. Sateii, A. Hamidi, M. Ahmadi, M. Salehi. 2014. Evaluation of lipid peroxidation and antioxidant enzymes activity in seeds of two soybean cultivars Accelerated by aging. Science and Technology of Seeds of Iran.1 (3):17-30. Mo'aveni, P., A. Voldabadi, A. Ebrahimi. 2010. Wheat. First Edition. University Press Azad Islamic Library Shahre-Ghods unit. Shahre-Ghods. (In Persian) Sairam, R.K., and D.C. Saxena. 2000. Oxidative stress and antioxidant in wheat genotypes,possible mechanism of water stress tolerance. Journal of Argonomy and Grop Science. 184: 55-61. Shaaban, M. 2016. Effect of aging on enzymatic and non-enzymatic antioxidant changes and biochemical characteristics in barley (Hordeum vulgare L.) seeds cv. Valfajr. Iranian J. Seed Sci. Res. 3(3): 79-93. (In Persian, with English Abstract) Simic, A., S. Sredojevic, M. Todorovic, L. Đukanovic, and C. Radenovic. 2004. Studies on the relationship between the content of total phenolics in exudates and germination ability of maize seed during accelerated aging. Seed Sci. Technol.32(1): 213-218. Slinkard, K., V.L.Singleton. 1977. Total phenol analysis; automation and comparison with manual methods. Am. J. Enol. Viticult. 28: 49-55. Soltani, A., M. Gholipoor, and E. Zeinali. 2006. Seed reserve utilization and seedling growth of wheat as affected by drought and salinity. J. Environ. Exp. Bot. 55: 195-200. Spanò, C., M.R. Castiglione, S. Bottega, and I. Grilli. 2004. Natural ageing of wheat seeds. J. Curr. Topics in Plant Biol. 5: 89–94. Srivall, B., and R. Khanna-Chopra. 2004. The developing reproductive sink induces oxidative stress to mediate nitrogen mobilization during monocarpic senescence in wheat. Biochem. Biophys. Res. Commun. 325: 198- 202. Stewart, R. R. C., and J. D. Bewley.1980. Lipid peroxidation associated with accelerated aging of soybean. Plant Physiol. 65: 245-248. Sung, J.M., T.L. Jeng. 1994. Lipid peroxidation and peroxide-scavenging enzymes associated with accelerated ageing of peanut seed. Physiol Plant. 91: 51–55. Veselovsky, V.A., and T.V. Veselova. 2012. Lipid peroxidation, carbohydrate hydrolysis, and amadori-maillard reaction at early stages of dry seed aging. Russ. J. Plant Physiol. 59: 763-770. Walters, C., D. Ballesteros, and V. Vertucci. 2010. Structural mechanics of seed deterioration: Standing the test of time. Plant Sci. 179: 565-573. | ||
|
آمار تعداد مشاهده مقاله: 481 تعداد دریافت فایل اصل مقاله: 500 |
||