Heybati, P., Lotfi, M., Eshaghi, A. (2021). Evaluation of cellular substrates used in research and production of biological products for infection with BVDV 1 & 2. , 34(4), 8-16. doi: 10.22092/vj.2020.351820.1763
P Heybati; M Lotfi; A. Eshaghi. "Evaluation of cellular substrates used in research and production of biological products for infection with BVDV 1 & 2". , 34, 4, 2021, 8-16. doi: 10.22092/vj.2020.351820.1763
Heybati, P., Lotfi, M., Eshaghi, A. (2021). 'Evaluation of cellular substrates used in research and production of biological products for infection with BVDV 1 & 2', , 34(4), pp. 8-16. doi: 10.22092/vj.2020.351820.1763
Heybati, P., Lotfi, M., Eshaghi, A. Evaluation of cellular substrates used in research and production of biological products for infection with BVDV 1 & 2. , 2021; 34(4): 8-16. doi: 10.22092/vj.2020.351820.1763

Evaluation of cellular substrates used in research and production of biological products for infection with BVDV 1 & 2

تحقیقات دامپزشکی و فرآورده‌های بیولوژیک
Article 2, Volume 34, Issue 4, January 2022, Pages 8-16    PDF (913.05 K)
Document Type: Full Research Paper
DOI: 10.22092/vj.2020.351820.1763
Authors
P Heybati1; M Lotfi* 2; A. Eshaghi2
1Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj
2Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
Abstract
Simultaneously with the expansion of vaccine production on cell culture media, identification and elimination of adventitious viruses infecting culture media of animal origin, as well as prevention of contamination of biological products from cell culture media to one of the main concerns of production Vaccine suppliers. The aim of this study was to investigate the possible contamination of cellular substrates used in research and production of biological products of Razi Vaccine and Serum Research Institute with BVD1 and BVD2 viruses. The address of this investigate was 44 samples from 44 cell lines with different passage numbers and storage dates in a total of 100 samples. After extracting RNA from the above cells, a screening test was performed using Real Time PCR and a pair of primers to identify all Pestivirus. In this experiment, the cytopathic biotype BVDV1a NADL was used as a positive control. Then the species and their subspecies were determined by RT-PCR method. Infection was observed in five cells Hek293, IBRS2, RBK, FLK, RK13, all cells except IBRS2 in BVDV1b typing. All of these cells are used in research and quality control of biological products.
Keywords
Virus; BVDV; Cell culture substrates; adventitious agents; Biological products
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