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Ibrahim, Ibrahim, Abdullah, Shamall, Karim, Abdulkarim. (1399). Determination of the optimal conditions of cloning Aerolysin gene from the common carp pathogen Aeromonas hydrophila in Escherichia coli BL21. سامانه مدیریت نشریات علمی, 19(5), 2258-2273. doi: 10.22092/ijfs.2020.122373
Ibrahim Ibrahim; Shamall Abdullah; Abdulkarim Karim. "Determination of the optimal conditions of cloning Aerolysin gene from the common carp pathogen Aeromonas hydrophila in Escherichia coli BL21". سامانه مدیریت نشریات علمی, 19, 5, 1399, 2258-2273. doi: 10.22092/ijfs.2020.122373
Ibrahim, Ibrahim, Abdullah, Shamall, Karim, Abdulkarim. (1399). 'Determination of the optimal conditions of cloning Aerolysin gene from the common carp pathogen Aeromonas hydrophila in Escherichia coli BL21', سامانه مدیریت نشریات علمی, 19(5), pp. 2258-2273. doi: 10.22092/ijfs.2020.122373
Ibrahim, Ibrahim, Abdullah, Shamall, Karim, Abdulkarim. Determination of the optimal conditions of cloning Aerolysin gene from the common carp pathogen Aeromonas hydrophila in Escherichia coli BL21. سامانه مدیریت نشریات علمی, 1399; 19(5): 2258-2273. doi: 10.22092/ijfs.2020.122373

Determination of the optimal conditions of cloning Aerolysin gene from the common carp pathogen Aeromonas hydrophila in Escherichia coli BL21

Iranian Journal of Fisheries Sciences
دوره 19، شماره 5، آذر 2020، صفحه 2258-2273    اصل مقاله (745.95 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22092/ijfs.2020.122373
نویسندگان
Ibrahim Ibrahim* 1؛ Shamall Abdullah1؛ Abdulkarim Karim2
1Fish Recourses and Aquatic Animals Department, College of Agriculture Salahaddin University-Erbil, Iraq.
2Biology Department, College of Science Salahaddin University-Erbil, Iraq.and Biology Department, College of science Knowledge University, Iraq.
چکیده
Aeromonas hydrophila is a gram-negative bacterium which associated with gastrointestinal diseases and septicaemia. This pathogenic bacterium has several virulence factors ranging from pili to the excreted protein which called (Aerolysin) with minor and major effects, respectively. Additionally, Aeromonas hydrophila is a widely distributed bacterium that commonly causes ulcers in cyprinid fish such as carps and secondary diseases in humans as well. In the present study, characteristics and haemolytic activities of the recombinant Aerolysin protein and optimal conditions for cloning are determined using the synthesized cloning/expression Aerolysin gene, assembled into the Escherichia coli BL21 (DE3) through pGEX-6P1 vector, using SDS-PAGE and western blotting techniques. The results declared that, the Aerolysin gene (1482 bp) was cloned by transforming the recombinant pGEX-6P1 vector into Escherichia coli BL21 (DE3) as a prokaryotic expression host. The SDS-PAGE results indicated that the estimated protein size was 54 KDa. Recombinant Aerolysin protein synthesis at both selected temperatures, 25°C and 37°C, indicated that 1 mM of isopropyl-β-D-thiogalactopyranoside (IPTG) was the optimum concentration for induction. However, the recombinant protein was unable to synthesize in the absence of IPTG inducer. Western blot analysis indicated the efficient sensitivity and specificity of the recombinant Aerolysin protein. In conclusion, the recombinant protein showed potential advantages for immunoassay approaches in order to decrease the economic losses caused by disease in the aquaculture industry.
کلیدواژه‌ها
Aeromonas hydrophila؛ Escherichia coli common Carp؛ Fish disease؛ Haemolytic activity؛ Recombinant protein؛ Aerolysin
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تعداد مشاهده مقاله: 187
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