| تعداد نشریات | 285 |
| تعداد نشریات سازمان | 83 |
| تعداد شماره‌ها | 3,086 |
| تعداد مقالات | 34,441 |
| تعداد مشاهده مقاله | 48,875,023 |
| تعداد دریافت فایل اصل مقاله | 79,333,239 |
بررسی تأثیر کیفیت اولیه بذر و شرایط انبارداری بر تغییرات بیوشیمیایی و فیزیولوژیکی بذر سویا | ||
| علوم و فناوری بذر ایران | ||
| مقاله 8، دوره 9، شماره 2 - شماره پیاپی 18، شهریور 1399، صفحه 101-118 اصل مقاله (1.01 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22034/ijsst.2019.123739.1232 | ||
| نویسندگان | ||
| سامان شیدایی* ؛ آیدین حمیدی؛ حسین صادقی؛ بیتا اسکویی | ||
| عضو هیأت علمی، مؤسسه تحقیقات ثبت و گواهی بذر و نهال، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران | ||
| چکیده | ||
| به‌منظور ارزیابی تغییرات بیوشیمیایی در طی زوال بذر سویا، تحقیقی به‌صورت آزمایش فاکتوریل بر پایه طرح کامل تصادفی اجرا گردید. فاکتورهای آزمایش شامل قوه نامیه در دو سطح قوه نامیه 80% و 90%، سه میزان رطوبت اولیه بذر شامل رطوبت پایین (10%)، رطوبت متوسط (12%) و رطوبت بالا (14%) و دو شرایط انبارکردن شامل انبار متداول نگهداری بذر سویا در مغان و انبار با دما و رطوبت کنترل شده در نظر گرفته شد. صفات درصد جوانه‌زنی، بنیه، میزان پروتئین، قندهای محلول، مالون دی آلدئید، فعالیت آنزیم‌های کاتالاز و پراکسیداز مورد سنجش قرار گرفتند. بررسی تغییرات بیوشیمیایی بذرهای زوال یافته در طی انبار کردن نشان داد که با افزایش زوال بذرها میزان قندهای محلول و درصد پروتئین کاهش یافت و نگهداری بذرها در شرایط نامناسب باعث کاهش معنی‌دار در میزان قندهای محلول و پروتئین کل بذر شد. همچنین با افزایش رطوبت بذر که افزایش زوال بذر را در پی داشت از محتوای قندهای محلول و پروتئین کل به-طور معنی‌داری کاسته شد. بین بذرهای با قوه نامیه اولیه متفاوت تفاوت معنی‌داری دیده شد و بذرهای با قوه نامیه اولیه 90 درصد به‌طور معنی‌داری محتوای قندهای محلول و درصد پروتئین بالاتری داشتند. همچنین با اندازه‌گیری میزان مالون دی-آلدئید و فعالیت آنزیم‌های کاتالاز و پراکسیداز مشخص گردید بین میزان زوال بذر با میزان مالون دی‌آلدئید و فعالیت آنزیم‌های تنظیف همبستگی بالایی مشاهده گردید. همراه با کاهش میزان جوانه‌زنی و کیفیت بذر سویا پس از انبارکردن، میزان پراکسیداسیون چربی‌ها افزایش و زوال بذر از فعالیت آنزیم‌های کاتالاز و پراکسیداز در طی انبارکردن کاست. | ||
| کلیدواژه‌ها | ||
| انبارداری؛ پروتئین؛ کاتالاز؛ مالون‌دی‌آلدئید؛ قندهای محلول | ||
| عنوان مقاله [English] | ||
| Evaluation of initial seed quality and storage conditions on biochemical and physiological changes of soybean seeds | ||
| نویسندگان [English] | ||
| S. Sheidaei؛ Aidin Hamidi؛ Hossein Sadeghi؛ Bita Oskouei | ||
| Seed and Plant Certification and Registration Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran. | ||
| چکیده [English] | ||
| A factorial experiment was conducted to evaluate the biochemical changes during the soybean seed deterioration based on a completely randomized design. The treatments consisted of germination in two levels of minimum and high quality, three initial seed moisture content (low, medium and high moisture), and two storage conditions were considered including the common storage in Moghan and the controlled storage condition. Indices of germination percentage, vigor and biochemical changes including protein content, soluble sugars, malondialdehyde, catalase and peroxidase enzymes were evaluated. The study of biochemical changes of deteriorated seeds during the storage showed that, with the increase of seeds’ deterioration, the soluble sugars and protein percentage decreased, as well. Moreover, seeds’ storage in poor condition caused a significant decrease in seeds’ soluble sugar and total protein content. Also, the increase of seed moisture was followed by further seed deterioration that significantly decreased the content of soluble sugars and total protein. Significant differences were observed between seeds with different initial germinations and they had significantly higher content of soluble sugars and protein percentages. Furthermore, measuring the amount of malondialdehyde and the activity of catalase and peroxidase enzymes indicated that there exists a significant correlation between the degree of seed deterioration with the level of malondialdehyde and the activity of scavenging enzymes. Overall, along with the decrease of germination and the quality of soybean seeds after the storage, the amount of lipids peroxidation increased and the seed deterioration decreased the activity of catalase and peroxidase enzymes during the storage. | ||
| کلیدواژه‌ها [English] | ||
| Catalase, Soluble sugar, Storage, Malondialdehyde | ||
| مراجع | ||
|
Abdul-baki, A.A., and J. D. Anderson. 1973. Vigor determination in soybean seed by multiplication. Crop Sci. 3: 630-633. AOAC (Association of Official Analytical Chemists). 1995. Official Methods of Analysis, 16th Ed. AOAC International, Gaithersburg, MD. USA. Bailly, C., A. Benamar, F. Corbineau, and D. Come. 1996. Changes in malondialdehyde content and in superoxide dismutase, catalase and glutathione reductase activities in sunflower seeds as related to deterioration during accelerated aging. Physiol. Plant. 97(1): 104-110 Basra, S.M.A., K.U. Rehman, and S. Iqbal. 2000. Cotton Seed Deterioration: Assessment of some Physiological and Biochemical Aspects. Int. J. Agric. Biol. 2 (3). Bernal, L., A. Camacho, and A. Caballo. 2000. Effect of seed aging in the enzymic antioxidant system of maize cultivars. PP. 151-160. In: M. Black, K.J. Bradford. And J. Vazqez-Ramos. Seed biology: advances and applications (eds). CABI publishing .UK. Bewley, J.D., K.J. Bradford, H.W. Hilhorst, and H. Nonogaki. 2013. Seeds: Physiology of Development, Germination and Dormancy. 3rd ed. Springer, New York. Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein day binding. Anal. Biochem. 72: 248-254. Cabrera, E.R., and H. Lansakara. 1995. Open storage of soybean seed in Mississippi. Tech. Bulletin Mississippi Agric. Forestry. No. 30776 Caja (343). Mississippi State University Chuansin, S., S. Vearasilp, S. Srichuwong, and E. Pawelzik. 2006. Selection of Packaging Materials for Soybean Seed Storage. Conference on International Agricultural Research for Development, University of Bonn. Davey, M.W., E. Stals, B. Panis, J. Keulemans, and R.L. Swennen. 2005. High-throughput of malondialdehyde in plant tissues. Anal. Biochem. 347: 201-207. Ghasemnezhad, A., and B. Honermeier. 2007. Influence of storage conditions on quality and viability of high and low oleic sunflower seeds. Intern. J. Plant Prod. 3(4): 41-50. Goel, A., and I.S. Sheoran. 2003. Lipid peroxidation and peroxide-scavenging enzymes in cotton seeds under natural ageing. Biol. Plant. 46(3): 429-434. Goel, A., A.K. Goel, and I.S. Sheoran. 2003. Changes in oxidative stress enzyme during artificial aging in cotton (Gossypium hirsutum L.) seed. J. Plant Physiol. 160: 1093-1100. Heatherly, L.G., M.M. Kenty, and T.C. Kilen. 1995. Effects of storage environment and duration on impermeable seed coat in soybean. Field Crops Res. 40: 57- 62. Hendry, G.A.F. 1993. Oxygen, free radical processes and seed longevity. Seed Sci, Res. 3: 141-153. Hosamani, J., M. Dadlani, I.M. Santha, M.A. Kumar, and S.R. Jacob. 2013. Biochemical Phenotyping of Soybean [Glycine max (L.) Merill] Genotypes to Establish the Role of Lipid Peroxidation and Antioxidant Enzymes in Seed Longevity. Agricultural Research. 2 (2): 119-126. ISTA. 2018. International Rules for Seed Testing. Zurichtstr.50. CH 8303. Bassersdorf, Switzerland, Edition 2018. Janmohammadi, M., Y. Fallahnezhad, M. Golshan, and H. Mohammadi. 2008. Controlled ageing for storaibility assessment and predicting seedling early growth of canola cultivars (Brassica napus L.). ARPN J. Agric. Biol. Sci. 3 (5&6): 22-26. Kapoor, N., A. Arya, M.A. Siddiqui, H. Kumar, and A. Amir. 2011. Physiological and Biochemical Changes During Seed Deterioration in Aged Seeds of Rice (Oryza sativa L.). Am. J. Plant Physiol. 6 (1): 28-35. Kapoor, R., A. Arya., M.A. Siddiqui, A. Amir, andH. Kumar. 2010. Seed Deterioration in Chickpea (Cicer arietinum L.) under Accelerated Ageing”, Asian J Plant Sci. 9 (3): 158-162. Kaur, S., A.K. Gupta, and N. Kaur. 1998. Gibberellic acid and kinetin partially reverse the effect of water stress on germination and seedling growth in chickpea. Plant Growth Regulation. 25: 29-33. Kibinza, S., D. Vinel, D. Côme., C. Bailly, andF. Corbineau. 2006. Sunflower seed deterioration as related to moisture content during ageing, energy metabolism and active oxygen species scavenging. Physiol. Plant. 128(3): 496-506. Krishnan, P., K.S. Chatitanya, S. Keshavkant, and S.C. Naithani. 2000. Changes in total protein and protease activity in dehydrating recalcitrant sal (Shorea robustd) seed. Silver Fennica. 34: 71-77. Krishnan, P., S. Nagarajan, M. Dadlani, and A.V. Moharir. 2003. Characterization of wheat (Triticum aestivum) and soybean (Glycine max) seeds under accelerated ageing conditions by Proton nuclear magnetic spectroscopy. Seed Sci. Technol. 31: 541- 550. Kuchlan, M.K. 2006. Identification of physical, physiological and biochemical factors and molecular analysis for longevity of soybean (Glycine max l. merill) seeds. Doctoral dissertation. Indian Agricultural Research Institute, New Delhi. Kurdilkle, M.B., G.T. Basavaraj, and H.N. Danapure. 1996. Storability of soybean (Glycine max (L.) Merr.) seed under ambient conditions. Karnataka J. Agri Sci. 9: 552-554. Locher, R., and P. Bucheli. 1998. Comparison of soluble sugar degradation in soybean seed under simulated tropical storage conditions. Crop Sci. 38 (5): 1229-1235. Ma, F., C. Ewa, M. Tasneem, C.A. Peterson, and M. Gijzen, 2004. Cracks in the palisade cuticle of soybean seed coats correlate with their permeability to water. Annals of Botany. 94: 213-228. Maguire, J.D. 1962. Speed of germination in selection and evaluation for seedling vigor. Crop Sci. 2: 176-177. McDonald, M.B. 2004. Orthodox seed deterioration and its repair. Pp. 273-304. In: Benech- Arnold, R.L. and R.L. Sanchez. (eds). Handbook of Seed Physiology. Food Product Press. Argentina. Morello, J.R., M.J. Motilva, M.J. Tovar, and M.P. Romero. 2004. Changes in commercial virgin olive oil (CV Arbequina) during storage with special emphasis on the phenolic fraction. J. Food Chem. 85: 357-364. Murty, U.M., P. Kumarand, and W.Q. Sun. 2003. Mechanisms of seed aging under different storage conditions for Vigna radiate wilczek. Lipid peroxidation, sugar hydrolysis, millard reactions and their relationship to glass state transition. J. Exp. Bot. 54: 1057-1067. Nkang, A., and E.O. Umoh. 1997. Six month storability of five soybean cultivars as influenced by stage of harvest, storage temperature and relative humidity. Seed Sci. Technol. 25: 93-99. Powel, A., and S. Mathews. 1992. Seed vigour and its measurement. PP: 98-109. Techninques in Seed Science and Technology, 2nd. ed. South Asain Publishers, New Delhi, India. Prochazkova, D., R.K. Sairam, G.C. Srivastava, and D.V. Singh. 2001. Oxidative stress and antioxidant activity as the basis of senescence in maize leaves. Plant Sci. 161: 765-771. Puntarulo, S., M. Galleano, R.A. Sanchez, and A. Boveris. 1991. Superoxide anion and hydrogen peroxide metabolism in soybean embryonic axes during germination. Biochim. Biophys. Acta. 1047: 277-283. Rastegar, Z., M. Sedighi, and S. Khomari. 2011. Effects of Accelerated Aging on Soybean Seed Germination Indexes at Laboratory Conditions. Not Sci Biol. 3: 126-129. Shelar, V.R., R.S. Shaikh, and A.S. Nikam. 2008. Soybean seed quality during storage: A review. Agric. Rev. 29(2): 125-131. Stan, O. 1997. The test of accelerated ageing to stablish the vigour potential in maize and sunflower hybrid seed. Romanian Agriculture Research. 7-8: 59-66. Sung, J.M. 1996. Lipid Peroxidation and peroxidase scavenging in soybean seeds during aging. Physiol. Plant. 97: 85–90. Sung, J.M., and C.C. Chiu. 1995. Lipid peroxidation and peroxide-scavenging enzymes of naturally aged soybean seed. Plant Sci. 110 (1): 45-52. Tatipata, A. 2009. Effect of seed moisture content packaging and storage period on mitochondria inner membrane of soybean seed. J. Agric. Technol. 5: 51-64. Vashisth, A., and S. Nagarajan. 2009. Germination Characteristics of Seeds of Maize (Zea mays L.) Exposed to Magnetic Fields under Accelerated Ageing Condition. J. Agric Physics. 9: 50-58. Walters, C., D. Ballesteros, and V.A. Vertucci. 2010. Structural mechanics of seed deterioration: Standing the test of time. Plant Sci. 179: 565–573. Malik. C.P. 2013. Seed deterioration: A Review. Int. J. Life Sci. Biotech. Pharm. Res. 2(3): 374-385. | ||
|
آمار تعداد مشاهده مقاله: 557 تعداد دریافت فایل اصل مقاله: 727 |
||