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تأثیر منابع کربن و نیتروژن بر رشد و اسپورزایی Bacillus thuringiensis var. tenebrionis | ||
| مهار زیستی در گیاه پزشکی | ||
| دوره 7، شماره 2 - شماره پیاپی 14، پاییز و زمستان 1398، صفحه 49-62 اصل مقاله (382.97 K) | ||
| نوع مقاله: مقاله علمی-پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/bcpp.2020.122492 | ||
| نویسندگان | ||
فاطمه صابری1؛ رسول مرزبان   2؛ مهدی ارجمند3؛ فرشید پژوم شریعتی1؛ امید توکلی4
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| 1دانشکده مهندسی شیمی، دانشگاه آزاد واحد علوم تحقیقات، تهران، ایران | ||
| 2مؤسسه تحقیقات گیاه پزشکی، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران | ||
| 3دانشکده مهندسی شیمی، دانشگاه آزاد واحد تهران جنوب، تهران، ایران | ||
| 4دانشکده مهندسی شیمی، پردیس دانشکده‌های فنی، دانشگاه تهران، تهران، ایران | ||
| چکیده | ||
| برای بومی‌سازی دانش تولید tenebrionisvar. Bacillus thuringiensis کنترل‌کننده آفات سخت بالپوش، اولین گام پس از جداسازی باکتری، بهینه‌سازی محیط تولید می‌باشد که در پروژه حاضر مورد مطالعه قرار گرفت. در این مطالعه ازB. thuringiensis var. tenebrionis–BN1 (Btt–BN1) به عنوان یک آفت‌کش بیولوژیک برای کنترل سوسک برگخوار نارون استفاده شد. هدف از بهینه کردن محیط کشت مطلوب، افزایش راندمان تولید آفت‌کش بیولوژیکB. thuringiensis با ارزش افزوده بالا می‌باشد که در این محیط کشت از ضایعات کشاورزی و صنایع غذایی (ملاس نیشکر، جو دوسر، سبوس گندم و شربت ذرت) به عنوان مواد اولیه ارزان قیمت استفاده شده است. برای بهینه‌سازی پارامترهای محیط کشت از طراحی آزمایش به روش پاسخ سطح RSM)) و با رویکرد طراحی مرکب مرکزی استفاده شد. متغیرهای مستقل شامل مقدار منبع کربن و مقدار منبع نیتروژن در 3 سطح غلظت (6/0 تا 1 درصد ملاس نیشکر، 3/0 تا 9/0 درصد جو دوسر، 3/0 تا 9/0 درصد سبوس گندم و 1 تا 5 درصد شربت ذرت) با پاسخ واحدهای تشکیل‌دهنده پرگنه (CFU) و درصد تلفات برگخوار نارون مورد بررسی قرار گرفت. نتایج بدست آمده در شرایط عملیاتی pH = 7 و دمای 30 درجه سلسیوس پس از 72 ساعت نشان داد که حداکثر میزان CFU برای حداقل غلظت جو دوسر (3/0%)، حداقل غلظت سبوس گندم (3/0%)، حداکثر غلظت ملاس نیشکر (1 %) و حداقل غلظت شربت ذرت (1 %) معادل spores.ml−18.3611 بدست آمد. همچنین نتایج زیست‌سنجی Btt–BN1 روی برگخوار نارون نشان داد دو غلظت 5/0 و 1 درصد به ترتیب 7/67% و 80% تلفات ایجاد می‌کند. | ||
| کلیدواژه‌ها | ||
| Bacillus thuringiensis var. tenebrionis؛ برگخوار نارون؛ راندمان تولید؛ بستر تولید؛ شرایط رشد | ||
| عنوان مقاله [English] | ||
| Influence of carbon and nitrogen sources on the growth and sporulation of Bacillus thuringiensis var. tenebrionis | ||
| نویسندگان [English] | ||
| Fatemeh Saberi1؛ Rasoul Marzban2؛ Mehdi Ardjmand3؛ Farshid Pajoum Shariati1؛ Omid Tavakoli4 | ||
| 1Department of Chemical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 2Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran | ||
| 3Department of Chemical Engineering, Tehran South Branch, Islamic Azad University, Tehran, Iran | ||
| 4School of Chemical Engineering, College of Engineering, University of Tehran, Tehran 14176, Iran | ||
| چکیده [English] | ||
| For knowledge localization of Bacillus thuringiensis (Bt) production as biological pest control, optimization of culture medium is necessary. In this study, Bacillus thuringiensis var. tenebrionis–BN1 (Btt–BN1) was used as a biological pesticide to control elm leaf beetles. The aim of optimizing the desire culture medium is to increase the production efficiency of Bt biopesticide using agricultural and food wastes as cheap raw materials. To optimize the parameters of the culture medium, experimental design of response surface methodology (RSM) was applied with a central composite design (CCD) approach. The parameters including the amount of carbon (sugar cane molasses, oats and wheat bran sources) and the amount of nitrogen (corn syrup source) at three levels (0.6 to 1 percent of sugar cane molasses, 0.3 to 0.9 percent of oat, 0.3 to 0.9 percent of wheat bran and 1 to 5 percent of corn syrup) were investigated with colony forming unit (CFU) response. The results obtained under operating conditions of pH = 7, temperature of 30oC after 72 hours. The results showed that the maximum CFU for the minimum concentration of oats (0.3%), the minimum concentration of wheat bran (0.3%), the maximum concentration of sugar cane molasses (1%) and the minimum concentration of corn syrup (1%) were equivalent to 8.36 11 spores.ml–1. In addition, the results of Btt–BN1 bioassay survey on elm leaf beetle showed that two concentrations of 0.5 and 1.0 percent cause 67.7% and 80.0% of mortality, respectively. | ||
| کلیدواژه‌ها [English] | ||
| Bacillus thuringiensis var. tenebrionis, elm leaf beetle, production efficiency, production substrate, growth conditions | ||
| مراجع | ||
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