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بررسی خاصیت ضد‌باکتریایی نانوذرات نقره بیوسنتز شده با استفاده از عصاره برگ زوفا (Hyssopus officinalis L.) تحت تأثیر تنش شوری | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 13، دوره 36، شماره 4 - شماره پیاپی 102، مهر و آبان 1399، صفحه 691-708 اصل مقاله (1.31 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2020.128037.2651 | ||
| نویسندگان | ||
| ایمان رسایی1؛ مریم قنادنیا* 2؛ سعید باغشاهی3 | ||
| 1کارشناسی ارشد بیوتکنولوژی کشاورزی، گروه بیوتکنولوژی کشاورزی، دانشکده کشاورزی و منابع طبیعی، دانشگاه بین‌المللی امام خمینی (ره)، قزوین، ایران | ||
| 2استادیار، گروه مهندسی علوم باغبانی، دانشکده کشاورزی و منابع طبیعی، دانشگاه بین‌المللی امام خمینی (ره)، قزوین، ایران | ||
| 3استاد، گروه مهندسی مواد، دانشکده فنی و مهندسی، دانشگاه بین‌المللی امام خمینی (ره)، قزوین، ایران | ||
| چکیده | ||
| شناسه دیجیتال (DOR): 98.1000/1735-0905.1399.36.691.102.4.1576.1610 پیشرفت‌های اخیر در علوم زیست‌شناسی به‌دلیل پایه‌ای بودن آنها برای تعدادی از علوم مرتبط مانند کشاورزی، پزشکی، داروسازی، بیوتکنولوژی و حتی بیونانوتکنولوژی اهمیت ویژه‌ای یافته است. در این تحقیق تأثیر تیمارهای مختلف شوری (0، 50، 100 و 150 میکرومولار) بر گیاه زوفا (Hyssopus officinalis L.) و ویژگی‌های نانوذرات نقره (Ag NPs) بیوسنتز شده توسط عصاره این گیاه بررسی شد. تغییر رنگ محلول‌ها، وجود پیک در ناحیه nm 450 اسپکتروفتومتری و نتایج حاصل از تفرق اشعه ایکس (XRD)، بیوسنتز نانوذرات نقره را تأیید کرد. تصاویر مربوط به میکروسکوپ الکترونی روبشی نشر میدانی (FESEM) بیشتر ذرات را کروی شکل نشان داد که اشکال زاویه‌دار نیز در تیمارهای 50 و 100 میکرومولار قابل مشاهده بودند. نتایج حاصل از دستگاه تبدیل طیف‌سنجی مادون‌قرمز (FTIR) عوامل شیمیایی مختلف مانند OH، CO، =CH و C=C را که در فرایند بیوسنتز شرکت کرده بودند نشان داد. غلظت 50 میکرومولار از نمک بیشترین تأثیر را بر افزایش متابولیت‌های گیاهان داشت. کوچکترین نانوذرات (nm25.3) مربوط به تیمار شاهد (کروی شکل) بود. برخی نانوذرات بیوسنتز شده توسط غلظت 150 میکرومولار با اندازه nm 34.2، زاویه‌دار بوده و بیشترین خاصیت ضد‌باکتریایی را نشان دادند. نانوذرات نقره بیوسنتز شده، تأثیر منفی بیشتری بر باکتری‌های گرم منفی نسبت به گرم مثبت داشتند. بیشترین خاصیت ضد‌باکتریایی را نانوذرات بیوسنتز شده توسط غلظت 150 میکرومولار نشان دادند. این نتایج در ادامه تحقیقات قبلی ما برای اولین بار تأثیر تیمارهای شوری را بر ویژگی‌های نانوذرات بیوسنتز شده توسط عصاره گیاه زوفا نشان داد. این نتایج می‌توانند زمینه جالب توجهی برای بیوسنتز نانوذرات نقره ایجاد کنند که بتوانند جایگزین مناسبی برای آنتی‌بیوتیک‌ها باشند. | ||
| کلیدواژه‌ها | ||
| زوفا (Hyssopus officinalis L.)؛ زیست‌شناسی؛ نانو ذرات نقره؛ NaCl | ||
| عنوان مقاله [English] | ||
| Assesment of antibacterial properties of silver nanoparticles biosynthesized using leaf extract of Hyssopus officinalis L. grown under salinity stress | ||
| نویسندگان [English] | ||
| I. Rasaee1؛ M. Ghannadnia2؛ S. Baghshahi3 | ||
| 1Department of Biotechnology, Imam Khomeini International University, Qazvin, Iran | ||
| 2Department of Horticultural Sciences Engineering, Faculty of Agriculture and Natural Resources, Imam Khomeini International University, Qazvin, Iran | ||
| 3Department of Materials Engineering, Imam Khomeini International University, Qazvin, Iran | ||
| چکیده [English] | ||
| Recent advances in the biological sciences have become particularly important because they are the basis for some related sciences such as agriculture, medicine, pharmacology, biotechnology, and even bionanotechnology. In this study, the effect of different salinity treatments (0, 50, 100, and 150 µM) on Hyssopus officinalis L. and the properties of silver nanoparticles) Ag NPs) biosynthesized using these under-salinity stress plants leaves extract were investigated. The color change of the solutions, surface plasmon resonance at 450 nm and X-ray diffraction pattern confirmed the biosynthesis of Ag NPs. Field emission scanning electron microscopy (FESEM) images showed that most of the nanoparticles were spherical, with few angular shapes visible in 50 and 100 µM treatments. Fourier-transform infrared spectroscopy (FTIR) results revealed the participant functional groups of the plant extract in the biosynthesis process such as OH, CO, =CH and C=C. The 50 µM salinity treatment had the highest effect on increasing plant metabolites. The smallest nanoparticles (25.3 nm and spherical) were related to the control treatment. Some nanoparticles biosynthesized using the extract obtained from 150 µM salinity treatment were angular in shape with 34.2 nm in size and showed the highest antibacterial properties. Gram-negative bacteria were more sensitive to Ag NPs than the gram-positive ones. These results, following our previous research, revealed for the first time the effect of salinity treatments on the properties of Ag NPs biosynthesized using hyssop extract. The present results can provide an interesting background for Ag NPs biosynthesis that can be a good alternative to antibiotics. | ||
| کلیدواژه‌ها [English] | ||
| Hyssopus officinalis L, biology, silver nanoparticles, NaCl | ||
| مراجع | ||
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