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Yousefian, Shirin, Ahmadi Nik, Fatemeh, Lohrasebi, Tahmineh, Mirshahvalad, Sharareh, Gharanjik, Shahrokh, Esfahani, Kasra. (1400). Investigation of Nitrogen and Phosphate Effect on Growth and Rosmarinic Acid Production in Transgenic Mentha aquatica Hairy Root Induction. سامانه مدیریت نشریات علمی, 10(2), 199-207. doi: 10.22092/jmpb.2020.351569.1249
Shirin Yousefian; Fatemeh Ahmadi Nik; Tahmineh Lohrasebi; Sharareh Mirshahvalad; Shahrokh Gharanjik; Kasra Esfahani. "Investigation of Nitrogen and Phosphate Effect on Growth and Rosmarinic Acid Production in Transgenic Mentha aquatica Hairy Root Induction". سامانه مدیریت نشریات علمی, 10, 2, 1400, 199-207. doi: 10.22092/jmpb.2020.351569.1249
Yousefian, Shirin, Ahmadi Nik, Fatemeh, Lohrasebi, Tahmineh, Mirshahvalad, Sharareh, Gharanjik, Shahrokh, Esfahani, Kasra. (1400). 'Investigation of Nitrogen and Phosphate Effect on Growth and Rosmarinic Acid Production in Transgenic Mentha aquatica Hairy Root Induction', سامانه مدیریت نشریات علمی, 10(2), pp. 199-207. doi: 10.22092/jmpb.2020.351569.1249
Yousefian, Shirin, Ahmadi Nik, Fatemeh, Lohrasebi, Tahmineh, Mirshahvalad, Sharareh, Gharanjik, Shahrokh, Esfahani, Kasra. Investigation of Nitrogen and Phosphate Effect on Growth and Rosmarinic Acid Production in Transgenic Mentha aquatica Hairy Root Induction. سامانه مدیریت نشریات علمی, 1400; 10(2): 199-207. doi: 10.22092/jmpb.2020.351569.1249

Investigation of Nitrogen and Phosphate Effect on Growth and Rosmarinic Acid Production in Transgenic Mentha aquatica Hairy Root Induction

Journal of Medicinal plants and By-products
دوره 10، شماره 2، آذر 2021، صفحه 199-207    اصل مقاله (685.21 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22092/jmpb.2020.351569.1249
نویسندگان
Shirin Yousefian1؛ Fatemeh Ahmadi Nik1، 2؛ Tahmineh Lohrasebi* 1؛ Sharareh Mirshahvalad1؛ Shahrokh Gharanjik2؛ Kasra Esfahani1
1Department of Plant Bioproducts, National Institude of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
2Department of Agricultural Biotechnology, Shahrood University of Technology, Shahrood, Iran
چکیده
Mentha aquatica L. as an important medicinal plant is known for its phenolic acids with pharmaceutical properties, especially rosmarinic acid. In order to obtain high amounts of secondary metabolites, the establishment of hairy root cultures through Agrobacterium rhizogenes infection is considered as a suitable alternative. In order to compare the abilities of A.rhizogenes strains to induce hairy roots, the leaf and shoot explants of M.aquatica were used for hairy root induction by five different bacterial strains (ATCC 15834, A13, R318, A4, 9534). Furthermore, the effect of phosphate, nitrogen, and reducing environmental acidity on growth and rosmarinic acid content of hairy roots were studied. The results indicated that the percentage of rooting in stem explants (50%) was higher than the leaves (42.6%). Among the bacterial strains, the strain A4 showed the highest rooting efficiency. It was also demonstrated that phosphate deficiency and increased phosphate levels significantly increased the rosmarinic acid content of hairy roots. Various effects on hairy root biomass were observed in response to different phosphate levels. However, increasing the amount of nitrogen did not affect the studied factors. It was concluded that different types of explants have different potentials of hairy root formation in interaction with bacterial strains.
کلیدواژه‌ها
Hairy Roots؛ Mentha Aquatic؛ Phosphate؛ Nitrogen؛ Rosmarinic Acid
مراجع
  1. Raja RR. Medicinally Potential Plants of Labiatae (Lamiaceae) Family: An Overview. Res J Med Plant. 2012;6:203-213.
  2. Jafari A, Kahrizi D, Mansouri M. Effects of plant growth regulators and explant on callus induction in pennyroyal (Mentha pulegium L.). Biharean Biol. 2016;10:134-136.
  3. Darvishi E, Kahrizi D, Bahraminejad S, Mansouri M. In vitro induction of α-pinene, pulegone, menthol, menthone and limonene in cell suspension culture of pennyroyal (Mentha pulegium). Cell Mol Biol. 2016;62:7-9.
  4. Darvishi E, Kazemi E, Kahrizi D, Bahraminejad S, Mansouri M, Chaghakaboudi SR, Khani Y. Optimization of callus induction in Pennyroyal (Mentha pulegium). Appl Biotechnol. Rep. 2014;1:97-100.
  5. Jäger AK, Almqvist JP, Vangsøe SAK, Stafford GI, Adsersen A, Van Staden J. Compounds from Mentha aquatica with Affinity to the GABA-Benzodiazepine Receptor. South African J Bot. 2007;73:518-21.
  6. Shekarchi M, Hajimehdipoor H, Saeidnia S, Gohari AR, Hamedani MP. Comparative Study of Rosmarinic Acid Content in Some Plants of Labiatae Family. Phcog Mag. 2012;8:37-41.
  7. Kim YB, Kim JK, Uddin MR, Xu H, Park WT, Tuan PA, et al. Metabolomics Analysis and Biosynthesis of Rosmarinic Acid in Agastache rugosa Kuntze Treated with Methyl Jasmonate. PLoS One. 2013;8:e64199.
  8. Petersen M, Simmonds MSJ. Rosmarinic acid. Phytochemistry. 2003;62:121-125.
  9. Fadel D. Study of phenolic compounds production in spearmint clones regenerated in vitro. PhD Thesis, Department of horticulture, Aristotle University of Thessaloniki, Greece. 2010.
  1. Pistelli L, Giovannini A, Ruffoni B, Bertoli A, Pistelli Luisa. Hairy root cultures for secondary metabolites production. Adv. Exp Med Biol. 2010;698:167-84.
  2. Zarei B, Kahrizi D, Mousavi SA, Nasrollahnezhad Ghomi AA. Agrobacterium rhizogense - mediated transformation of Atropa belladonna. J. Agric. Biotech. 2013;5:59-67.
  3. Motamedi J, Zebarjadi A, Kahrizi D, Salmanian A H. Transformation of bacterial mutated aroA gene to safflower (Carthamus tinctorius L.) via Agrobacterium-mediated method. Modern Gen J (MGJ). 2012;7:57-63.
  4. Zárate R, Cequier-Sánchez E, Rodríguez C, Dorta-Guerra R, El Jaber-Vazdekis N, Ravelo ÁG. Improvement of Polyunsaturated Fatty Acid Production in Echiu acanthocarpum Transformed Hairy Root Cultures by Application of Different Abiotic Stress Conditions. ISRN Biotechnol. 2013;169510.
  5. Sambrook J. and Russell Dw. “Molecular Cloning: A Laboratory Manual.” Cold Spring Harbor Laboratory Press. 2001.
  6. Thwe A, Valan Arasu M, Li X, Park CH, Kim SJ, Al-Dhabi NA, Park SU. Effect of Different Agrobacterium rhizogenes Strains on Hairy Root Induction and Phenylpropanoid Biosynthesis in Tartary Buckwheat (Fagopyrum tataricum Gaertn). Front Microbiol. 2016;7:318.
  7. Srivastava S, Conlan XA, Adholeya A, Cahill DM. Elite hairy roots of Ocimum basilicum as a new source of rosmarinic acid and antioxidants. Plant Cell, Tissue Organ Cult. 2016;126:19-32.
  8. Esmaeili F, Shiran BJ, Fallahi H, Mirakhorli N, Budak H, and Martínez-Gómez P. In silico search and biological validation of microRNAs related to drought response in peach and almond. Funct Integr Genomics. 2016;17:189-201.
  9. Dorman HJD, Koşar M, Kahlos K, Holm Y, Hiltunen R. Antioxidant properties and composition of aqueous extracts from Mentha species, hybrids, varieties, and cultivars. J Agric Food Chem. 2003;51:4563-4569.
  10. Kanatt SR, Chander R, Sharma A, Antioxidant potential of mint (Mentha spicata L.) in radiation-processed lamb meat. Food Chem. 2007;100:451-458.
  11. Cirlini M, Mena P, Tassotti M, Herrlinger KA, Nieman KM, Dall’Asta C, et al. Phenolic and Volatile Composition of a Dry Spearmint (Mentha spicata L.) Extract. Molecules. 2016;21:1007.
  12. Md Setamam N, Jaafar Sidik N, Abdul Rahman Z, Che Mohd Zain CR. Induction of hairy roots by various strains of Agrobacterium rhizogenes in different types of Capsicum species explants. BMC Res. Notes. 2014;7:414.
  13. Zhang S, Yan Y, Wang B, Liang Z, Liu Y, Liu F, Qi Z. Selective responses of enzymes in the two parallel pathways of rosmarinic acid biosynthetic pathway to elicitors in Salvia miltiorrhiza hairy root cultures. J Biosci Bioeng. 2014;117:645-651.
  14. Lee SY, Xu H, Kim YK, Park SU. Rosmarinic acid production in hairy root cultures of Agastache rugosa Kuntze. World J Microbiol Biotechnol. 2008;24:969-972.
  15. Chen H, Chen F, Zhang YL, Song JY. Production of lithospermic acid B and rosmarinic acid in hairy root cultures of Salvia miltiorrhiza. J Ind Microbiol Biotechnol. 1999;22:133-138.
  16. Bauer N, Kiseljak D, Jelaska S. The effect of yeast extract and methyl jasmonate on rosmarinic acid accumulation in Coleus blumei hairy roots. Biol Plant. 2009; 53:650-656.
  17. Giri A, Banerjee S, Ahuja PS, Giri CC. Production of hairy roots in Aconitum heterophyllum wall. using Agrobacterium rhizogenes. Vitr. Cell. Dev. Biol. – Plant. 1997;33:280-284.
  18. Bonhomme V, Laurain-Mattar D, Fliniaux MA. Effects of the rol C gene on hairy root: induction development and tropane alkaloid production by Atropa belladonna. J Nat Prod. 2000;63:1249-1252.
  19. Argôlo AC, Charlwood BV, Pletsch M. The regulation of solasodine production by Agrobacterium rhizogenes-transformed roots of Solanum aviculare. Planta Med. 2000;66:448-451.
  20. Park WT, Baskar TB, Yeo SK, Park N Il, Park JS, Park S. Response of Different Agrobacterium Rhizogenes Strains for in vitro Hairy Root Induction and Accumulation of Rosmarinic Acid Production in Agastache Rugosa. Online J Biol Sci. 2017;17:136-142.
  21. Malik S. Production of Plant Derived Natural Compounds through Hairy Root Culture, Springer. 2017.
  22. Nourozi E, Hosseini B, Hassani A. A reliable and efficient protocol for induction of hairy roots in Agastache foeniculum. Biologia (Bratisl). 2014;69:870-879.
  23. Marsh Z, Yang T, Nopo-Olazabal L, Wu S, Ingle T, Joshee N, Medina-Bolivar F. Effect of light, methyl jasmonate and cyclodextrin on production of phenolic compounds in hairy root cultures of Scutellaria lateriflora. Phytochemistry. 2014;107:50-60.
  24. Wilczańska-Barska A, Królicka A, Głód D, Majdan M, Kawiak A, Krauze-Baranowska M. Enhanced accumulation of secondary metabolites in hairy root cultures of Scutellaria lateriflora following elicitation. Biotechnol. Lett. 2012;34:1757-1763.
  25. Liu L, Yang D, Xing B, Zhang H, Liang Z. Salvia castanea Hairy Roots are More Tolerant to Phosphate Deficiency than Salvia miltiorrhiza Hairy Roots Based on the Secondary Metabolism and Antioxidant Defenses. Mol A J Synth Chem. Nat. Prod. Chem. 2018;23.
  26. Ahn JC, Chong WS, Kim YS, Hwang B. Optimization of the Sucrose and Ion Concentrations for Saikosaponin Production in Hairy Root Culture of Bupleurum falcatum. Biotechnol. Bioprocess Eng. 2006;11.
  27. Liu S, Zhong JJ. Phosphate effect on production of ginseng saponin and polysaccharide by cell suspension cultures of Panax ginseng and Panax quinquefolium. Process Biochem. 1998;33:69-74.
  28. Uozumi N, Makino S, Kobayashi T. 20-Hydroxyecdysone production in Ajuga hairy root controlling intracellular phosphate content based on kinetic model. J Ferment Bioeng. 1995;80:362-368.
  29. Długosz M, Markowski M, Pączkowski C. Source of nitrogen as a factor limiting saponin production by hairy root and suspension cultures of Calendula officinalis L. Acta Physiol Plant. 2018;40:35.
  30. Ilieva M, Pavlov A. Rosmarinic acid production by Lavandula vera MM cell suspension culture: nitrogen effect. World J Microbiol Biotechnol. 1999;15:711-714.
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