Immunity Evaluation of an Experimental Designed Nanoliposomal Vaccine Containing FMDV Immunodominant Peptides

Heshmati, L.; Rezayat, S. M.; Madani, R.; Emami, T.; Jaafari, M. R.; Golchinfar, F.; Kazemi, M.; Azizmi Dezfouli, S. M.

doi:10.22092/ari.2021.352498.1566

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	Immunity Evaluation of an Experimental Designed Nanoliposomal Vaccine Containing FMDV Immunodominant Peptides
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نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2021.352498.1566
نویسندگان
L. Heshmati¹؛ S. M. Rezayat²^{، 3}^{، 4}؛ R. Madani ⁵^{، 6}؛ T. Emami ⁵؛ M. R. Jaafari⁷^{، 8}؛ F. Golchinfar⁵؛ M. Kazemi⁹؛ S. M. Azizmi Dezfouli ¹⁰
¹Department of Medical Nanotechnology, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
²Department of Pharmacology & Toxicology, Faculty of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University of Tehran, Iran (IAUPS)
³Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
⁴Department of Nanotechnology in medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
⁵Department of Proteomics and Biochemistry, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran
⁶Department of Microbiology, School of Specialized Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
⁷Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
⁸Department of Pharmaceutical Nanotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
⁹Department of biology, faculty of sciences, university of Guilan, Rasht, Iran
¹⁰Department of Foot and Mouth Vaccine Production, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, IranExtension Organization (AREEO), Karaj, Iran
چکیده
Foot-and - mouth disease (FMD) is a highly contagious viral disease affecting cloven-hoofed animals. Iran is an endemic region of foot-and - mouth disease. Today, conventionally attenuated and killed virus vaccines are being used worldwide. In Iran, animals have been vaccinated every 105 days with an inactivated FMD vaccine. Although commercially available FMD vaccines are effective, they provide short-term immunity requiring regular boosters. A new FMD vaccine is needed to improve immunization, safety and long-term immune responses. A synthetic peptide vaccine is one of the safe and important vaccines. Peptide vaccine has low immunogenicity and applying strong adjuvant is necessary. nanoliposomes as new adjuvants could be used to improve the immune response. In the current study, Nano liposomal carrier as an adjuvant containing two immunodominant synthetic FMDV peptides was selected. The efficacy of the vaccines was evaluated in guinea pigs. Indirect ELISA was used to detect FMDV-specific IgG in the serum of vaccinated guinea pigs.
کلیدواژه‌ها
Guinean pigs؛ adjuvants؛ ELISA؛ Encapsulation Efficiency
عنوان مقاله [English]
Immunity Evaluation of an experimental designed nanoliposomal vaccine containing FMDV immunodominant peptides
چکیده [English]
Foot-and - mouth disease (FMD) is a highly contagious viral disease affecting cloven-hoofed animals. The particular virus that causes FMD disease is called FMD virus that is a member of the Aphthovirus genus in the Picornaviridae family. FMD virus has a single strain positive RNA genome with a length of 8500 nt with one ORF which are trapped in an icosahedral capsid protein. This virus genome doesn't have proofreading property which tends to highly mutagenesis. It has seven serotypes, such as, O, A, ASIA, SAT1, SAT2 and C and a lot of subtypes. Iran is an endemic region of foot-and - mouth disease. Vaccination of susceptible animals with inactivated whole-virus vaccine is the only way to control the epidemic in many developing countries .Today, conventionally attenuated and killed virus vaccines are being used worldwide. In Iran, animals have been vaccinated every 105 days with an inactivated FMD vaccine. Although commercially available FMD vaccines are effective, they provide short-term immunity requiring regular boosters. A new FMD vaccine is needed to improve immunization, safety and long-term immune responses. A synthetic peptide vaccine is one of the safe and important vaccines. Peptide vaccine has low immunogenicity and applying strong adjuvant is necessary. nanoliposomes as new adjuvants could be used to improve the immune response. In the current study, Nano liposomal carrier as an adjuvant containing two immunodominant synthetic FMDV peptides was selected. The efficacy of the vaccines was evaluated in guinea pigs. Indirect ELISA was used to detect FMDV-specific IgG in the serum of vaccinated guinea pigs.
کلیدواژه‌ها [English]
adjuvants, Guinean pigs, ELISA, IgG, Encapsulation Efficiency

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Immunity Evaluation of an Experimental Designed Nanoliposomal Vaccine Containing FMDV Immunodominant Peptides