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ارزیابی فنوتیپی، بیماری‌زایی و مولکولی جدایههای استرپتومایسس بیمارگر سیبزمینی در خراسان رضوی | ||
| آفات و بیماری های گیاهی | ||
| دوره 88، شماره 2 - شماره پیاپی 111، اسفند 1399، صفحه 227-239 اصل مقاله (649.98 K) | ||
| نوع مقاله: بیماری‌شناسی گیاهی | ||
| شناسه دیجیتال (DOI): 10.22092/jaep.2020.127992.1306 | ||
| نویسندگان | ||
| فریده بی سیار؛ ساره بقائی راوری* ؛ عصمت مهدیخانی مقدم | ||
| گروه گیاه پزشکی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران | ||
| چکیده | ||
| اسکب معمولی یکی از بیماری‌های مهم باکتریایی سیبزمینی است که تولید بهینه این محصول اقتصادی را به‍طور‍فراگیر محدود می‍سازد. متعاقب نمونه برداری سال‍های 1396 و 1397، پرگنه‍های پودری با شکل‍شناسی جنس استرپتومایسس خالص‍سازی و از نظر ویژگی های فنوتپی، بیماری‌زایی و ردیابی ژنهای مستقر روی جزیره بیماری‌زایی شامل txtAB،nec1 و tomA ارزیابی شدند. شکل‍شناسی پرگنه جدایه‍ها روی محیط عصاره مخمر-مالت-آگار متنوع و جدایههای بیمارگر علایم عدم‍جوانه‍زنی، نکروز وکوتولگی را روی گیاهچه‍های تربچه نشان داده و از نظر فنوتیپی به شش گروه تفکیک شدند. از 20 جدایه بیمارگر روی تربچه، 11 جدایه قادر به ایجاد نکروز ویا فرورفتگی در ورقه‍های سیبزمینی و هشت جدایه علایم کلروز و یا نکروز را روی برگ‍های توتون پس‍از‍گذشت 10 روز ایجاد نمودند. نتایج بیماری‌زایی در شرایط گلخانه، به‍صورت زخم‍های سطحی و یا برجسته با شاخص شدت لکه بین یک تا چهار مشخص شد. قطعه 700 جفت‍بازی nec1، در شش جدایه از مجموع هشت جدایه منتخب ردیابی شد. درحالی‌که ژنهای txt AB و tom A به‍ترتیب در جدایههای JR-12 و TM-29 تکثیر شدند. بر‍اساس نتایج توالی‍یابی 16SrRNA، جدایه TM-29 به‍عنوان Streptomyces bottropensis و جدایه JR-12 به‍عنوان Streptomyces sp. مشخص شد. به‍نظر می‍رسد جدایههای خراسان رضوی، دارای فاکتور بیماری‌زایی به‍غیر از تاکستومین A هستند که برای تأیید آن، انجام آزمایشات تکمیلی ضرورت دارد. | ||
| کلیدواژه‌ها | ||
| استرپتومایسس؛ بیماری‌زایی؛ پرآزاری؛ شناسایی مولکولی | ||
| عنوان مقاله [English] | ||
| Phenotypic, pathogenicity and molecular evaluation of potato pathogenic Streptomyces strains in Razavi-Khorasan | ||
| نویسندگان [English] | ||
| Farideh Bisayar؛ Sareh Baghaee-Ravari؛ Esmat Mahdikhani-Moghaddam | ||
| Department of Crop Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran | ||
| چکیده [English] | ||
| Common scab is one of the most important bacterial diseases of potato which restricts the optimal production of this economical crop. Following sampling in 2018-2019, the powdery colonies with Streptomyces morphology were purified and evaluated based on phenotypic features, pathogenicity and detecting the genes on Pathogenicity Island including txtAB, nec1 and tomA. The colony morphology of the strains on malt yeaste extract agar was variable and pathogenic strains showed symptoms such as no germination, dwarfing and necrosis on radish seedlings which were divided into six phenotypic groups. 11 out of 20 pathogenic strains on radish, were capable of causing necrosis or pitting on potato slices and eight of them showed chlorosis or necrosis on tobacco leaves after 10 days. The results of pathogenicity including superficial or raised lesions with the lesion severity index of 1-4 were recorded in greenhouse conditions. The 700 bp fragment was detected in six out of eight selected strains. While, txtAB and tomA were amplified in JR-12 and TM-29, respectively. Based on 16rSRNA sequencing results, JR-12 and TM-29 strains were characterized as Streptomyces bottropensis and Streptomyces sp < em>. Respectively.It seems that the Razavi-Khorasan strains have a virulence factor other than Thaxtomine A, which needs further experiments to confirm. | ||
| کلیدواژه‌ها [English] | ||
| Molecular characterization, pathogenicity, Streptomyces, virulence | ||
| مراجع | ||
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