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Feng, Junchang, Guo, Xiangrui, Chang, Xulu, Zhang, Yuru, Meng, Xiaolin, Lu, Ronghua, Huang, Mengyuan, Nie, Guoxing, Zhang, Jianxin. (1399). Characterization of a high-affinity polyclonal antibody against the Cyprinus carpio (Linnaeus, 1758) intestinal tight junction protein occluding. سامانه مدیریت نشریات علمی, 20(2), 343-357. doi: 10.22092/ijfs.2021.123785
Junchang Feng; Xiangrui Guo; Xulu Chang; Yuru Zhang; Xiaolin Meng; Ronghua Lu; Mengyuan Huang; Guoxing Nie; Jianxin Zhang. "Characterization of a high-affinity polyclonal antibody against the Cyprinus carpio (Linnaeus, 1758) intestinal tight junction protein occluding". سامانه مدیریت نشریات علمی, 20, 2, 1399, 343-357. doi: 10.22092/ijfs.2021.123785
Feng, Junchang, Guo, Xiangrui, Chang, Xulu, Zhang, Yuru, Meng, Xiaolin, Lu, Ronghua, Huang, Mengyuan, Nie, Guoxing, Zhang, Jianxin. (1399). 'Characterization of a high-affinity polyclonal antibody against the Cyprinus carpio (Linnaeus, 1758) intestinal tight junction protein occluding', سامانه مدیریت نشریات علمی, 20(2), pp. 343-357. doi: 10.22092/ijfs.2021.123785
Feng, Junchang, Guo, Xiangrui, Chang, Xulu, Zhang, Yuru, Meng, Xiaolin, Lu, Ronghua, Huang, Mengyuan, Nie, Guoxing, Zhang, Jianxin. Characterization of a high-affinity polyclonal antibody against the Cyprinus carpio (Linnaeus, 1758) intestinal tight junction protein occluding. سامانه مدیریت نشریات علمی, 1399; 20(2): 343-357. doi: 10.22092/ijfs.2021.123785

Characterization of a high-affinity polyclonal antibody against the Cyprinus carpio (Linnaeus, 1758) intestinal tight junction protein occluding

Iranian Journal of Fisheries Sciences
دوره 20، شماره 2، خرداد 2021، صفحه 343-357    اصل مقاله (479.61 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22092/ijfs.2021.123785
نویسندگان
Junchang Feng؛ Xiangrui Guo؛ Xulu Chang؛ Yuru Zhang؛ Xiaolin Meng؛ Ronghua Lu؛ Mengyuan Huang؛ Guoxing Nie؛ Jianxin Zhang*
College of Fisheries
چکیده
Tight junction protein, occludin, plays an important role in intestinal health of fish. To study the function of occludin in the intestinal barrier at the protein level, a rabbit occludin polyclonal antibody was prepared against heterologously expressed Cyprinus carpio A fragment of the occludin gene containing antigenic determinants was first ligated into the pET-21a, which is an expression vector and transformed into E. coli BL21 (DE3) strain. Expression of the target fusion protein was induced by isopropyl-beta-D-thiogalactopyranoside (IPTG). The purified fusion protein was used as an antigen to immunize New Zealand long-eared rabbits (Oryctolagus cuniculus) through ear margin vein and subcutaneous injection to obtain rabbit anti-carp polyclonal antibodies against occludin. Enzyme-linked immunosorbent assay (ELISA) was used to evaluate the antibody titre, and the antibody was used to determine the distribution and expression of occludin in the intestine of carp after infection with Aeromonas hydrophila. The target fusion protein had a molecular weight of approximately 31.7 ku, the antibody titre was 2.4 × 106, and the integrity of occludin protein was lower in various intestinal segments after A. hydrophila infection. The results indicated that the prepared antibody had a high titre, affinity, and specificity and can be applied to study the expression and distribution of occludin in C. carpio. The availability of this polyclonal antibody laid the foundation for the systematic study of the intestinal barrier of Cyprinus carpio . Additionally, this polyclonal antibody could also be used for explorative studies of the biological function of occludin in other fishes.
 
کلیدواژه‌ها
Cyprinus carpio؛ Occludin؛ Prokaryotic expression؛ Antibody titre؛ Immunohistochemistry
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