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Inspection the Linkage between Bovine GDF9 SNPs and Calving Rate (Superovulation) in Holstein Friesians Cows | ||
| Archives of Razi Institute | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 12 خرداد 1400 اصل مقاله (610.72 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2021.354310.1632 | ||
| نویسندگان | ||
S. T Rasheed 1؛ L. S Younis  2؛ Q. M Aboud 2
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| 1Veterinary College, Tikrit University | ||
| 2Veterinary College, Fallujah University | ||
| چکیده | ||
| This study prepared to explore the relation between Growth Differentiation Factor 9 (GDF9) genotypes and calving rate, Follicle-stimulating hormone (FSH) and Estradiol (E2) in Iraqi Holstein Friesians breed. Fifteen blood samples collected from a mother of DZTB (with high calving rate records), another blood sample collected from 15 SB cows, the DNA extracted and six primers designed for PCR and sequencing analysis. The FSH and E2 level tested through estrus phase for the two groups (10 n for each group). The sequence evaluation revealed that presence 2 single nucleotide polymorphisms (SNPs) in exon II; A (1109) T and G (1133) A. The genotypic frequency for mutant genotypes was higher significantly (P<0.01) in DZTB cows (with calving rate) than wild at the same loci. On the other hand, the wild genotypes recorded higher significant increment (P<0.01) for SB cows when compared with mutant genotypes in the same loci. Moreover, a significant rise (P<0.05) was reported in E2 and FSH levels for DZTB cows and mutant genotypes (P<0.01) against SB cows and wild genotypes respectively in 0 and 24 h of estrus phase. Non-significant differences recorded in E2 concentration between the same genotypes at same period. In conclusion, the GDF9 exon II SNPs increase the calving rate in Holstein Friesians cows. The blood FSH and E2 concentrations are higher in the mother of DZTB may control the superovulation. Finally, these SNPs can be considered as markers to accelerate the breeding programs and used in embryo transfer and in vitro embryo production for Iraqi Holstein Friesians cows breed. | ||
| کلیدواژه‌ها | ||
| Calving rate؛ Dizygotic twinning؛ GDF9 polymorphism؛ FSH؛ Estradiol | ||
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آمار تعداد مشاهده مقاله: 3 تعداد دریافت فایل اصل مقاله: 8 |
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