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Alwaeely, F. A, Madlum, K. N, Alsaadi, M. A. (1400). The Immunomodulatory Effect of Propolis on Foxp3 Gene Expression in Human Peripheral Blood Mononuclear Cells Stimulated in vitro with pseudomonas aeruginosa Ag.. سامانه مدیریت نشریات علمی, (), -. doi: 10.22092/ari.2021.355389.1678
F. A Alwaeely; K. N Madlum; M. A Alsaadi. "The Immunomodulatory Effect of Propolis on Foxp3 Gene Expression in Human Peripheral Blood Mononuclear Cells Stimulated in vitro with pseudomonas aeruginosa Ag.". سامانه مدیریت نشریات علمی, , , 1400, -. doi: 10.22092/ari.2021.355389.1678
Alwaeely, F. A, Madlum, K. N, Alsaadi, M. A. (1400). 'The Immunomodulatory Effect of Propolis on Foxp3 Gene Expression in Human Peripheral Blood Mononuclear Cells Stimulated in vitro with pseudomonas aeruginosa Ag.', سامانه مدیریت نشریات علمی, (), pp. -. doi: 10.22092/ari.2021.355389.1678
Alwaeely, F. A, Madlum, K. N, Alsaadi, M. A. The Immunomodulatory Effect of Propolis on Foxp3 Gene Expression in Human Peripheral Blood Mononuclear Cells Stimulated in vitro with pseudomonas aeruginosa Ag.. سامانه مدیریت نشریات علمی, 1400; (): -. doi: 10.22092/ari.2021.355389.1678

The Immunomodulatory Effect of Propolis on Foxp3 Gene Expression in Human Peripheral Blood Mononuclear Cells Stimulated in vitro with pseudomonas aeruginosa Ag.

Archives of Razi Institute
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 15 مرداد 1400  XML اصل مقاله (550.05 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2021.355389.1678
نویسندگان
F. A Alwaeely email ؛ K. N Madlum؛ M. A Alsaadi
College of Medicine, University of Babylon, Hilla, IRAQ
چکیده
Immune balance during infection is critical for both supporting the body's immune system's defense and preventing an overly aggressive immune response. Foxp3, a transcription factor of regulatory T cells, plays critical roles in balancing the body's immune system. Propolis has been shown to have an effect on Foxp3 expression. The aim of this study was to verify the effect of propolis extracts on in vitro Foxp3 gene expression in peripheral blood mononuclear cells (PBMCs) stimulated with P. aeruginosa Ag. In this study, a total of 20 apparently healthy volunteers were included, with 10 males and 10 females ranging in age from 20-40 year-olds. Five ml of blood were drawn from each participant in order to assess Foxp3 gene expression in PBMCs using density gradient lymphoprep and stimulated with pseudomonas aeruginosa LPS in vitro. The samples allocated into four distinct groups as follows; LPS stimulated PBMCs, Ethanol extracted propolis EEP+LPS stimulated PBMCs, water extracted propolis WEP+LPS stimulated PBMCs, and PBMCs  as a control group. The Foxp3 gene expression level were estimated, in all the four groups following a period of 48 h of cultivation, by Real time PCR technique uses SYBR green dye. Results of the study indicated that propolis had a great effect on the mRNA Foxp3 expression. Both ethanol extracted propolis and water extracted propolis had immunomodulatory effects through the Foxp3 mRNA expression, both the EEP and WEP could significantly inhibit Foxp3 mRNA gene expression by human PBMCs after stimulation with pseudomonas Ag in vitro. Propolis exhibits an immunoregulatory effect with the same effect with ethanol and water extracts on Foxp3 mRNA gene expression.
کلیدواژه‌ها
Immunomodulatory؛ Propolis؛ Foxp3؛ Gene expression
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