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Alwaeely, F. A, Madlum, K. N, Alsaadi, M. A. (1400). Immunomodulatory Effect of Propolis on Foxp3 Gene Expression in Human Peripheral Blood Mononuclear Cells Stimulated in vitro with Pseudomonas Aeruginosa Ag. سامانه مدیریت نشریات علمی, 76(4), 887-894. doi: 10.22092/ari.2021.355389.1678
F. A Alwaeely; K. N Madlum; M. A Alsaadi. "Immunomodulatory Effect of Propolis on Foxp3 Gene Expression in Human Peripheral Blood Mononuclear Cells Stimulated in vitro with Pseudomonas Aeruginosa Ag". سامانه مدیریت نشریات علمی, 76, 4, 1400, 887-894. doi: 10.22092/ari.2021.355389.1678
Alwaeely, F. A, Madlum, K. N, Alsaadi, M. A. (1400). 'Immunomodulatory Effect of Propolis on Foxp3 Gene Expression in Human Peripheral Blood Mononuclear Cells Stimulated in vitro with Pseudomonas Aeruginosa Ag', سامانه مدیریت نشریات علمی, 76(4), pp. 887-894. doi: 10.22092/ari.2021.355389.1678
Alwaeely, F. A, Madlum, K. N, Alsaadi, M. A. Immunomodulatory Effect of Propolis on Foxp3 Gene Expression in Human Peripheral Blood Mononuclear Cells Stimulated in vitro with Pseudomonas Aeruginosa Ag. سامانه مدیریت نشریات علمی, 1400; 76(4): 887-894. doi: 10.22092/ari.2021.355389.1678

Immunomodulatory Effect of Propolis on Foxp3 Gene Expression in Human Peripheral Blood Mononuclear Cells Stimulated in vitro with Pseudomonas Aeruginosa Ag

Archives of Razi Institute
مقاله 18، دوره 76، شماره 4، آذر و دی 2021، صفحه 887-894    اصل مقاله (416.85 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2021.355389.1678
نویسندگان
F. A Alwaeely* ؛ K. N Madlum؛ M. A Alsaadi
College of Medicine, University of Babylon, Hilla, Iraq
چکیده
Immune balance during infection is critical for both supporting the defense of the immune system of the body and preventing an overly aggressive immune response. Foxp3, a transcription factor of regulatory T cells, plays a critical role in balancing the immune system of the body. Propolis has been shown to affect Foxp3 expression. This study aimed to verify the effect of propolis extracts on in vitro Foxp3 gene expression in peripheral blood mononuclear cells (PBMCs) stimulated with Pseudomonas aeruginosa Ag. In this study, a total of 20 apparently healthy volunteers were included, with 10 males and 10 females within the age range of 20-40 years old. Five ml of blood were drawn from each participant to assess Foxp3 gene expression in PBMCs using density gradient lymphoprep and stimulated with P.aeruginosa lipopolysaccharide (LPS) in vitro. The samples were divided into four distinct groups as follows: LPS stimulated PBMCs, ethanol-extracted propolis (EEP) + LPS stimulated PBMCs, and water-extracted propolis (WEP) + LPS stimulated PBMCs and PBMCs as the control group. The Foxp3 gene expression level was estimated in all four groups following a period of 48 h of cultivation by real-time polymerase chain reaction technique using SYBR green dye. Results of the study indicated that propolis had a great effect on the mRNA Foxp3 expression. Both EEP and WEP had immunomodulatory effects through the Foxp3 mRNA expression, both the EEP and WEP could significantly inhibit Foxp3 mRNA gene expression by human PBMCs after stimulation with pseudomonas Ag in vitro. Propolis exhibited an immunoregulatory effect which was the same with ethanol and water extracts on Foxp3 mRNA gene expression.
کلیدواژه‌ها
Foxp3؛ Gene expression؛ Immunomodulatory؛ Propolis
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مراجع
  1. Belkaid Y. Regulatory T cells and infection: a dangerous necessity. Nat Rev Immunol. 2007;7(11):875-88.
  2. Wohlfert E, Belkaid Y. Role of endogenous and induced regulatory T cells during infections. J Clin Immunol. 2008;28(6):707-15.
  3. Williams LM, Rudensky AY. Maintenance of the Foxp3-dependent developmental program in mature regulatory T cells requires continued expression of Foxp3. Nat Immunol. 2007;8(3):277-84.
  4. Sudrajad H, Mudigdo A, Purwanto B, Setiamika M. Ethanol extract of propolis decreases the Interleukin-8 (IL-8) expression and blood Malondialdehyde (MDA) level in otitis media rat model induced by Pseudomonas aeruginosa. Bali Medical Journal. 2020;9(2):7
  5. Sanz-Rubio D, Sanz A, Varona L, Bolea R, Forner M, Gil AV, et al. Forkhead Box P3 Methylation and Expression in Men with Obstructive Sleep Apnea. Int J Mol Sci. 2020;21(6).
  6. Morgan ME, van Bilsen JH, Bakker AM, Heemskerk B, Schilham MW, Hartgers FC, et al. Expression of FOXP3 mRNA is not confined to CD4+CD25+ T regulatory cells in humans. Hum Immunol. 2005;66(1):13-20.
  7. Sakaguchi S, Mikami N, Wing JB, Tanaka A, Ichiyama K, Ohkura N. Regulatory T Cells and Human Disease. Annu Rev Immunol. 2020;38:541-66.
  8. Anjum SI, Ullah A, Khan KA, Attaullah M, Khan H, Ali H, et al. Composition and functional properties of propolis (bee glue): A review. Saudi J Biol Sci. 2019;26(7):1695-703.
  9. Tani H, Hikami S, Takahashi S, Kimura Y, Matsuura N, Nakamura T, et al. Isolation, Identification, and Synthesis of a New Prenylated Cinnamic Acid Derivative from Brazilian Green Propolis and Simultaneous Quantification of Bioactive Components by LC-MS/MS. J Agric Food Chem. 2019;67(44):12303-12.
  10. Pagliarone AC, Missima F, Orsatti CL, Bachiega TF, Sforcin JM. Propolis effect on Th1/Th2 cytokines production by acutely stressed mice. J Ethnopharmacol. 2009;125(2):230-3.
  11. Tani H, Hasumi K, Tatefuji T, Hashimoto K, Koshino H, Takahashi S. Inhibitory activity of Brazilian green propolis components and their derivatives on the release of cys-leukotrienes. Bioorg Med Chem. 2010;18(1):151-7.
  12. Shaha A, Mizuguchi H, Kitamura Y, Fujino H, Yabumoto M, Takeda N, et al. Effect of Royal Jelly and Brazilian Green Propolis on the Signaling for Histamine H1 Receptor and Interleukin-9 Gene Expressions Responsible for the Pathogenesis of the Allergic Rhinitis. Biol Pharm Bull. 2018;41(9):1440-7.
  13. Szliszka E, Mertas A, Czuba ZP, Krol W. Inhibition of Inflammatory Response by Artepillin C in Activated RAW264.7 Macrophages. Evid Based Complement Alternat Med. 2013;2013:735176.
  14. Mikami N, Tani H, Kawakami R, Sugimoto A, Sakaguchi S, Ikuta T. Brazilian green propolis promotes TNFR2 expression on regulatory T cells. Food Sci Nutr. 2021;9(6):3200-8.
  15. Takeda K, Nagamatsu K, Okumura K. A water-soluble derivative of propolis augments the cytotoxic activity of natural killer cells. J Ethnopharmacol. 2018;218:51-8.
  16. Paviani LC, Dariva C, Marcucci MC, Cabral FA. Supercritical Carbon Dioxide Selectivity to Fractionate Phenolic Compounds from the Dry Ethanolic Extract of Propolis. J Food Process Eng. 2010;33(1):15-27.
  17. Contari G. Process for the propolis extract Preparation. Apicolt Mod. 1987;78:50-147.
  18. Carlone GM, Thomas ML, Rumschlag HS, Sottnek FO. Rapid microprocedure for isolating detergent-insoluble outer membrane proteins from Haemophilus species. J Clin Microbiol. 1986;24(3):330-2.
  19. Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77-89.
  20. Kolbe U, Yi B, Poth T, Saunders A, Boutin S, Dalpke AH. Early Cytokine Induction Upon Pseudomonas aeruginosa Infection in Murine Precision Cut Lung Slices Depends on Sensing of Bacterial Viability. Front Immunol. 2020;11:598636.
  21. Tone Y, Furuuchi K, Kojima Y, Tykocinski ML, Greene MI, Tone M. Smad3 and NFAT cooperate to induce Foxp3 expression through its enhancer. Nat Immunol. 2008;9(2):194-202.
  22. Hozzein WN, Badr G, Al Ghamdi AA, Sayed A, Al-Waili NS, Garraud O. Topical application of propolis enhances cutaneous wound healing by promoting TGF-beta/Smad-mediated collagen production in a streptozotocin-induced type I diabetic mouse model. Cell Physiol Biochem. 2015;37(3):940-54.
  23. Benson A, Murray S, Divakar P, Burnaevskiy N, Pifer R, Forman J, et al. Microbial infection-induced expansion of effector T cells overcomes the suppressive effects of regulatory T cells via an IL-2 deprivation mechanism. J Immunol. 2012;188(2):800-10.
  24. Kassim M, Yusoff KM, Ong G, Sekaran S, Yusof MY, Mansor M. Gelam honey inhibits lipopolysaccharide induced endotoxemia in rats through the induction of heme oxygenase-1 and the inhibition of cytokines, nitric oxide, and high-mobility group protein B1. Fitoterapia. 2012;83(6):1054-9.
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