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Isolation, Extraction, Purification and Characterization of Fibrinolytic Enzyme from Pseudomonas aeruginosa and Estimate the Molecular Weight of the Enzyme | ||
| Archives of Razi Institute | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 18 شهریور 1400 اصل مقاله (577.47 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2021.355745.1716 | ||
| نویسندگان | ||
B H. Jasim  ؛ E H. Ali
| ||
| Biotechnology Branch, Departments of Applied Sciences, University of Technology, Iraq | ||
| چکیده | ||
| Pseudomonas aeruginosa was isolated from injuries of wounds and burned patient to ensure that the isolate was belonging to Pseudomonas aeruginosa, several tests are performed such as staining techniques, biochemical test, morphological, Vitek 2 system and sensitivity test. The gram stain test results showed rod pink gram negative bacteria, these findings served as a proof that the isolate was belong to Pseudomonas aeruginosa. Growth optimization of bacterial were done by assessing different combination of pH and temperatures. It is revealed that the best conditions for increasing the number of bacteria achieved at 37°C with bacterial number of 5.53×108 and pH 6 with bacterial number of 5.87×108. Fibrinolytic enzyme is agent that lysis fibrin clots. This fibrinolytic factor has prospective use to treat cardiovascular diseases, such as stroke and heart attack. Cardiovascular diseases attracted worldwide attention for its elevation morbidity and mortality. Fibrinolytic enzyme was extracted by centrifugation at 10000 × g for 10 min in 4°C, supernatant was taking and the pellet having bacterial cells was discarding. Purification of the fibrinolytic enzyme was achieved by using salt precipitation, ion exchange and gel filtrations chromatographic techniques. The results showed that, the gel filtration chromatography has optimal specific activity and purification fold at 562.6 U/ml, the final specific activity of the purified enzyme increased 4.1 times. The molecular weight of the fibrinolytic enzyme was determined as 26 kDa by gel filtration chromatography. The purified fibrinolytic enzyme has optimum activity in pH 7 and 40°C. The pH stability for the enzyme activity found in pH 6–7 and range of 10°C from 30° to 40°C. | ||
| کلیدواژه‌ها | ||
| Characterization؛ Isolation؛ Identification؛ Pseudomonas Aeruginosa؛ Fibrinolytic enzyme | ||
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آمار تعداد مشاهده مقاله: 128 تعداد دریافت فایل اصل مقاله: 109 |
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