اخبار و اعلانات

 آمار

تعداد نشریات284
تعداد نشریات سازمان82
تعداد شماره‌ها3,043
تعداد مقالات34,036
تعداد مشاهده مقاله46,421,594
تعداد دریافت فایل اصل مقاله77,487,827
Ahmed Hasan, S, Fakhraddin Raheem, T, Mohammed Abdulla, H. (1400). Phenotypic, Antibiotyping, and Molecular Detection of Klebsiella Pneumoniae Isolates from Clinical Specimens in Kirkuk, Iraq. سامانه مدیریت نشریات علمی, 76(4), 1061-1067. doi: 10.22092/ari.2021.355770.1721
S Ahmed Hasan; T Fakhraddin Raheem; H Mohammed Abdulla. "Phenotypic, Antibiotyping, and Molecular Detection of Klebsiella Pneumoniae Isolates from Clinical Specimens in Kirkuk, Iraq". سامانه مدیریت نشریات علمی, 76, 4, 1400, 1061-1067. doi: 10.22092/ari.2021.355770.1721
Ahmed Hasan, S, Fakhraddin Raheem, T, Mohammed Abdulla, H. (1400). 'Phenotypic, Antibiotyping, and Molecular Detection of Klebsiella Pneumoniae Isolates from Clinical Specimens in Kirkuk, Iraq', سامانه مدیریت نشریات علمی, 76(4), pp. 1061-1067. doi: 10.22092/ari.2021.355770.1721
Ahmed Hasan, S, Fakhraddin Raheem, T, Mohammed Abdulla, H. Phenotypic, Antibiotyping, and Molecular Detection of Klebsiella Pneumoniae Isolates from Clinical Specimens in Kirkuk, Iraq. سامانه مدیریت نشریات علمی, 1400; 76(4): 1061-1067. doi: 10.22092/ari.2021.355770.1721

Phenotypic, Antibiotyping, and Molecular Detection of Klebsiella Pneumoniae Isolates from Clinical Specimens in Kirkuk, Iraq

Archives of Razi Institute
مقاله 36، دوره 76، شماره 4، آذر و دی 2021، صفحه 1061-1067    اصل مقاله (441.99 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2021.355770.1721
نویسندگان
S Ahmed Hasan* 1؛ T Fakhraddin Raheem2؛ H Mohammed Abdulla2
1Department of Biology, College of Education of Pure Science Kirkuk University, Iraq
2Basic Science Department, Faculty of Dentistry, Al. Kitab University, Iraq
چکیده
Klebsiella pneumoniae is globally responsible for hospital- and community-acquired infections. This study aimed to determine the prevalence of K. pneumoniae and investigate the antibiotic resistance profile among clinical specimens at Azadi Teaching Hospital in Kirkuk, Iraq, and detect the rpoB gene for molecular identification of  K. pneumoniae in comparison with phenotypic and biochemical methods. In total, 250 clinical specimens were collected from patients in Azadi Teaching Hospital in Kirkuk, Iraq, between January 2018 and May 2018. The isolates were identified by morphologic and biochemical testing. Kirby-Bauer disk diffusion method was used in the antibiotics susceptibility test. Following that, 19 (7.6%) K. pneumoniae isolates were isolated from 250 clinical specimens (5 [5.61%] and 14 [8.69%] from males and females, respectively), and most of them (n=12; 11.76%) were isolated from the age group of 10-35 years old. The isolates were reported high resistance towards various types of antibiotics, especially penicillins and cephalosporins. In contrast, K. pneumoniae showed very low resistance to imipenem and amikacin (5.26% and 10.52%, respectively). The range of multidrug-resistant K. pneumoniae isolates in this study was estimated at 100%. In gene detection, all isolates in this study showed PCR product with 108 bp by K. pneumonia specific primer (rpoB). Developed antibiotic policies and regular surveillance of antibiotic susceptibility patterns may help to overcome the indiscriminate use of antibiotics that is a major cause of the emergence of drug resistance among pathogens.
کلیدواژه‌ها
K. pneumoniae؛ Multidrug Resistance؛ rpoB Gene
سایر فایل های مرتبط با مقاله
مراجع
  1. Munita JM, Arias CA. Mechanisms of antibiotic resistance. Microbiol Spectr. 2016;4(2):4.2. 15.
  2. Wyres KL, Holt KE. Klebsiella pneumoniae as a key trafficker of drug resistance genes from environmental to clinically important bacteria. Curr Opin Microbiol. 2018;45:131-9.
  3. Bengoechea JA, Sa Pessoa J. Klebsiella pneumoniae infection biology: living to counteract host defences. FEMS Microbiol Rev. 2019;43(2):123-44.
  4. Prestinaci F, Pezzotti P, Pantosti A. Antimicrobial resistance: a global multifaceted phenomenon. Pathog Glob Health. 2015;109(7):309-18.
  5. Ashurst JV, Dawson A. Klebsiella Pneumonia: StatPearls Publishing, Treasure Island (FL); 2020.
  6. Seifi K, Kazemian H, Heidari H, Rezagholizadeh F, Saee Y, Shirvani F, et al. Evaluation of biofilm formation among Klebsiella pneumoniae isolates and molecular characterization by ERIC-PCR. Jundishapur J Microbiol. 2016;9(1).
  7. Börner T, Aleynikova AY, Zubo YO, Kusnetsov VV. Chloroplast RNA polymerases: Role in chloroplast biogenesis. Biochim Biophys Acta Bioenerg. 2015;1847(9):761-9.
  8. Goldstein BP. Resistance to rifampicin: a review. J Antibiot 2014;67(9):625-30.
  9. Beyene G, Tsegaye W. Bacterial uropathogens in urinary tract infection and antibiotic susceptibility pattern in jimma university specialized hospital, southwest ethiopia. Ethiop J Health Sci. 2011;21(2):141-6.
  10. Zamani A, Mashouf RY, Namvar AME, Alikhani MY. Detection of magA Gene in Klebsiella spp. Isolated from clinical samplesdetection of magA. Iran J Basic Med Sci 2013;16(2):173.
  11. Magiorakos A-P, Srinivasan A, Carey R, Carmeli Y, Falagas M, Giske C, et al. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clin Microbiol Infect. 2012;18(3):268-81.
  12. Kadum SM. Colistin Susceptibility in Carbapenem Resistant Klebsiella Pneumoniae and their Ability of Biofilm Formation. Iraqi J Sci. 2020:517-27.
  13. Namratha K, Sreeshma P, Subbannayya K, Dinesh P, Champa H. Characterization and antibiogram of Klebsiella spp. isolated from clinical specimen in a rural teaching hospital. Sch J App Med Sci. 2015;3(2E):878-83.
  14. Nirwati H, Sinanjung K, Fahrunissa F, Wijaya F, Napitupulu S, Hati VP, et al. Biofilm formation and antibiotic resistance of Klebsiella pneumoniae isolated from clinical samples in a tertiary care hospital, Klaten, Indonesia. BMC Proc. 2019;13(11):20
  1. Hasan SA, Abass KS. Prevalence of Gram Negative Bacteria Isolated from Patients with Burn Infection and their Antimicrobial Susceptibility Patterns in Kirkuk City, Iraq. Indian J Public Health Res Dev 2019;10(8).
  2. Hasan SA, Najati AM, Abass KS. Prevalence and antibiotic resistance of “pseudomonas aeruginosa” isolated from clinical samples in Kirkuk City, Iraq. Eurasia J Biosci. 2020;14(1):1821-5.
  3. Al-Rubaye DS, Hamza HM, Abdulrahman TR. Genotyping of Klebsiella spp. isolated from different clinical sources. Iraqi J Sci. 2016;57(3B):1937-51.
  4. Manjula G, N, CM GNK, Patil S, Gaddad S, Shivannavar C. antibiotic susceptibility pattern of ESbetaL producing Klebsiella pneumoniae isolated from urine samples of pregnant women in Karnataka. J Clin Diagn Res. 2014;8(10).
  5. Wu H, Moser C, Wang H-Z, Høiby N, Song Z-J. Strategies for combating bacterial biofilm infections. Int J Oral Sci. 2015;7(1):1-7.
  6. Alves MS, Dias RCdS, de Castro ACD, Riley LW, Moreira BM. Identification of clinical isolates of indole-positive and indole-negative Klebsiella spp. J Clin Microbiol. 2006;44(10):3640-6.
21.          Hadi Z. Detection of extended-spectrum beta-lactamases of Escherichia coli and Klebsiella spp. isolated from patients with significant bacteriuria in Najaf: M. Sc. Thesis. College of Medicine. Kufa University; 2008. 

آمار
تعداد مشاهده مقاله: 2,066
تعداد دریافت فایل اصل مقاله: 2,737


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب