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آمار
| تعداد نشریات | 278 |
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| تعداد شماره‌ها | 2,175 |
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| تعداد مشاهده مقاله | 13,619,608 |
| تعداد دریافت فایل اصل مقاله | 10,947,749 |
Evolution of Mechanical Stress (In vitro) on Properties of Granulocytes in Acute Lymphoblastic Leukemia | ||
| Archives of Razi Institute | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 18 شهریور 1400 اصل مقاله (307.45 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2021.355848.1727 | ||
| نویسندگان | ||
M Yu. Skorkina  ؛ T S. Shevchenko؛ A S. Taranenko؛ E S. Shentseva؛ L R. Zakirova
| ||
| Belgorod State University, 85, Pobedy St., Belgorod, 308015, Russia | ||
| چکیده | ||
| Acute lymphoid leukemia affects the lymph cells or lymphocytes that make up the lymph tissue and has an acute process, which bone marrow cells do not mature properly. The processes by which cells convert mechanical stimuli into biochemical signals are called mechanical transitions, resulting in the sensation of specific cellular responses. In the performed study the functional properties of granulocytes of the patients with acute lymphoblastic leukaemia were investigated at the model of mechanical stress in vitro.The experimental part of the work was executed using blood from patients with ALL (n=30) being treated in the Hematological Department of Belgorod Region Hospital. The age of patients was 18-45 years old. Sample blood was performed in all patients who underwent a standard course of chemotherapy. Blood sampling was carried out by venepunctures and collected into the vacuum tubes Vacuette K3E. Blood samples from each experimental group were divided into two parts including the control and the experiment. The injection model of mechanical stress was used for the experimental group in vitro. The ATP concentration increased at 1.8 times compared with the control. Under the influence of mechanical stress Young’s module which numerically characterizes the rigidity of the granulocytes’ plasmalemma decreased by 54.4% (p<0.05). The surface potential of plasmalemma between control and experimental samples in the patients with ALL didn’t differ significantly, however, the adhesive force between erythrocyte and granulocyte increased by 30.7% (p<0.05). In the test of the osmotic load was found the increase of cell’s volume during the all-time incubation. At the initial second of incubation, the use of membrane reserve by granulocytes increased by 47% (p<0.05). The obtained data point to the regulatory role of ATP molecules in intercellular signaling and add modern ideas about mechanisms of intercellular interaction in the microvasculature at the development of leukemia. Moreover, the presented results can be taken into account at the development of new pharmacological immunocorrectors. | ||
| کلیدواژه‌ها | ||
| Adhesive properties of biomembranes؛ Atomic force microscopy؛ Granulocytes؛ Potential surface؛ Young’s module | ||
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آمار تعداد مشاهده مقاله: 3 |
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