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مقایسه آثار اسانس دو گونه گیاه Origanum vulgare L. و Origanum majorana L. بر رده سلول سرطانی BCL-1 و سلول‌های سیستم ایمنی | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 6، دوره 37، شماره 5 - شماره پیاپی 109، آذر و دی 1400، صفحه 781-794 اصل مقاله (941.64 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2021.352596.2898 | ||
| نویسندگان | ||
| فاطمه مشهدی1؛ مجید قربانی نهوجی2؛ رویا یارایی* 3 | ||
| 1دانشجوی کارشناسی ارشد، گروه ایمونولوژی، دانشکده پزشکی، دانشگاه شاهد، تهران، ایران | ||
| 2استادیار، سیستماتیک گیاهی، مرکز تحقیقات گیاهان دارویی، پژوهشکده گیاهان دارویی جهاد دانشگاهی، کرج، ایران | ||
| 3دانشیار، گروه ایمونولوژی، دانشکده پزشکی، دانشگاه شاهد، تهران، ایران | ||
| چکیده | ||
| گیاهان خانواده نعناعیان منبع غنی از ترکیب‌های دارویی با خواص ضدسرطان هستند. در بسیاری از تحقیقات تنها به خواص ضد سرطانی این ترکیب‌ها پرداخته شده است و به سمّیت آنها بر سلول‌های سالم توجهی نشده است. این مطالعه با هدف ارزیابی و مقایسه اثرهای سمّی اسانس دو گونه از جنس مرزنجوش (خانواده نعناعیان) شامل Origanum vulagare L. و Origanum majorana L. بر رده سلول سرطانی BCL-1 و لنفوسیت (ایمنی اکتسابی) و ماکروفاژ (ایمنی ذاتی) نرمال در شرایط In vitro طراحی شده است. نمونه گیاهان تهیه و اسانس روغنی با روش تقطیر با آب آماده شد. سپس آنالیز اسانس‌ها با دستگاه‌های GC و GC.MS انجام گردید. رده سلول سرطانی BCL-1، لنفوسیت و ماکروفاژ نرمال با غلظت‌های مختلف اسانس‌ها به مدت 24 ساعت کشت داده شد. حیات سلولی و مسمومیت سلولی تحت تأثیر اسانس‌های روغنی از طریق سنجش MTT و LDH تعیین شد. نتایج GC.MS تنوع ترکیب‌های دو اسانس را نشان می‌دهد، به‌طوری که تنها دارای 12 جزء مشترک می‌باشد. دو اسانس در محدوده غلظت 1-01/0 درصد (O. vulgare 47-79% و O. majorana 30-82%) موجب مهار رشد سلول سرطانی BCL-1 می‌شود (IC50 اسانس O. vulgare: 01/0% و IC50 اسانس O. majorana: 03/0%). هر دو اسانس در بالاترین غلظت (1%) فعالیت حیاتی سلول‌های ایمنی را کاهش نمی‌دهند. همچنین با توجه به نتایج آزمون LDH هیچ‌یک از غلظت‌های مورد آزمایش دو اسانس اثر سمّی بر لنفوسیت و ماکروفاژ نداشتند. اسانس گیاهان O. vulgare و O. majorana کاندید مناسبی برای درمان سرطان می‌باشند، زیرا علاوه‌بر داشتن خاصیت ضدسرطانی قوی، در سلول‌های نرمال سیستم ایمنی آسیبی ایجاد نمی‌کنند. | ||
| کلیدواژه‌ها | ||
| Origanum؛ ضدسرطان؛ BCL-1؛ لنفوسیت؛ ماکروفاژ | ||
| عنوان مقاله [English] | ||
| Effects of essential oils of Origanum vulgare L. and Origanum majorana L. on cancer cells line BCL-1 and immune system cells | ||
| نویسندگان [English] | ||
| F. Mashhadi1؛ M. Ghorbani Nohooji2؛ R. Yaraee3 | ||
| 1M.Sc. student, Department of Immunology, Faculty of Medicine, Shahed University, Tehran, Iran | ||
| 2Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran | ||
| 3Department of Immunology, Faculty of Medicine, Shahed University, Tehran, Iran | ||
| چکیده [English] | ||
| Plants from the fam. lamiaceae are the rich sources of medicinal compounds with the anticancer properties. In many studies, only the anticancer properties of these compounds have been investigated and their toxicity on the healthy cells has not been addressed. The present study was designed to evaluate and compare the toxic effects of Origanum vulgare L. and O. majorana L. essential oils on the cancer cells line BCL-1 and normal lymphocytes and macrophages under in vitro conditions. The plant samples essential oils were extracted using the hydro-distillation method and analyzed by GC and GC/MS. The cancer cells line BCL-1 and normal lymphocytes and macrophages were cultured with the various concentrations of essential oils for 24h. The viability and cells toxicity under the influence of essential oils were determined by the MTT and LDH assays. The GC/MS results showed that the two essential oils had only 12 common components. Both essential oils inhibited the growth of cancer cells line BCL-1 (O. vulgare, 47-79% and O. majorana, 30-82%) at a concentration of 0.01-1% (O. vulgare essential oil IC50: 0.01% and O. majorana essential oil IC50: 0.03%). The essential oils at the highest concentration (1%) did not decrease the immune cells viability. Also, according to the LDH test results, none of the concentrations of both essential oils had a toxic effect on the normal lymphocytes and macrophages. Overall, based on the present study results, the O. vulgare and O. majorana essential oils could be recommended as good candidates for the cancer treatment because they had no cytotoxic effect on the normal immune system cells in addition to having the strong anticancer properties. | ||
| کلیدواژه‌ها [English] | ||
| Origanum, anti-cancer, BCL-1, lymphocyte, macrophage | ||
| مراجع | ||
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