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Hazim Abdul Hameed, D, Hussein Ali, E. (1400). Extraction and Purification of Extracellular L-Glutamate Oxidase from Streptomyces. سامانه مدیریت نشریات علمی, 76(4), 769-779. doi: 10.22092/ari.2021.355928.1738
D Hazim Abdul Hameed; E Hussein Ali. "Extraction and Purification of Extracellular L-Glutamate Oxidase from Streptomyces". سامانه مدیریت نشریات علمی, 76, 4, 1400, 769-779. doi: 10.22092/ari.2021.355928.1738
Hazim Abdul Hameed, D, Hussein Ali, E. (1400). 'Extraction and Purification of Extracellular L-Glutamate Oxidase from Streptomyces', سامانه مدیریت نشریات علمی, 76(4), pp. 769-779. doi: 10.22092/ari.2021.355928.1738
Hazim Abdul Hameed, D, Hussein Ali, E. Extraction and Purification of Extracellular L-Glutamate Oxidase from Streptomyces. سامانه مدیریت نشریات علمی, 1400; 76(4): 769-779. doi: 10.22092/ari.2021.355928.1738

Extraction and Purification of Extracellular L-Glutamate Oxidase from Streptomyces

Archives of Razi Institute
مقاله 8، دوره 76، شماره 4، آذر و دی 2021، صفحه 769-779    اصل مقاله (927.87 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2021.355928.1738
نویسندگان
D Hazim Abdul Hameed* ؛ E Hussein Ali
Department of Biotechnology, Faculty of Applied Sciences, University of Technology, Iraq
چکیده
The bacterial isolates Streptomyces were obtained from the soil and cultivated in a wheat bran medium, which was used to produce the L–glutamate oxidase enzyme. The extracellular enzyme was then extracted using a cooling centrifugation process to obtain the filtrate that represents the crude enzyme. Afterward, the enzyme purification processes were carried out which included precipitation with ammonium sulfate as a preliminary purification step followed by dialysis to remove the salts. Next, ion-exchange chromatography and gel filtration were used to finish the purification process, and the enzyme activity was determined for each purification step. The results of purification of L-glutamate oxidase enzyme from streptomyces using ammonium sulfate showed that the specific activity was 8.25 units/mg protein with a saturation ratio of 60%. Moreover, the results of purification using a dialysis tube indicated that the specific activity was 9.5 units/mg protein. In addition, the result of purification using diethylaminoethyl cellulose ion column revealed that the specific activity was 25 unit/mg protein and the results of purification using gel filtration showed that the specific activity was 56 units/mg protein which was the best step in the purification process due to high specific activity of the enzyme. The optimum temperature and pH for the activity and stability of the enzyme were tested. Based on the findings, the optimum temperature for the activity of the enzyme was 37 °C. In addition, it was found that the optimum temperature range for the stability of the enzyme was 30-50 °C. Besides, the optimum pH for the activity was 7.0 and the optimum pH range for the enzyme stability was 5.0-7.0.
کلیدواژه‌ها
L-glutamate oxidase؛ L-glutamate؛ Streptomyces؛ Specific activity
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