اخبار و اعلانات

 آمار

تعداد نشریات284
تعداد نشریات سازمان82
تعداد شماره‌ها3,063
تعداد مقالات34,185
تعداد مشاهده مقاله46,825,345
تعداد دریافت فایل اصل مقاله77,814,728
Sulaiman, A. H, Ghassan, Z. I, Omar, T. N. (1400). Biochemical Evaluation of Carbonic Anhydrase and Some Antioxidant Markers in Patients with Diabetes Complications. سامانه مدیریت نشریات علمی, 77(1), 169-178. doi: 10.22092/ari.2021.356308.1817
A. H Sulaiman; Z. I Ghassan; T. N Omar. "Biochemical Evaluation of Carbonic Anhydrase and Some Antioxidant Markers in Patients with Diabetes Complications". سامانه مدیریت نشریات علمی, 77, 1, 1400, 169-178. doi: 10.22092/ari.2021.356308.1817
Sulaiman, A. H, Ghassan, Z. I, Omar, T. N. (1400). 'Biochemical Evaluation of Carbonic Anhydrase and Some Antioxidant Markers in Patients with Diabetes Complications', سامانه مدیریت نشریات علمی, 77(1), pp. 169-178. doi: 10.22092/ari.2021.356308.1817
Sulaiman, A. H, Ghassan, Z. I, Omar, T. N. Biochemical Evaluation of Carbonic Anhydrase and Some Antioxidant Markers in Patients with Diabetes Complications. سامانه مدیریت نشریات علمی, 1400; 77(1): 169-178. doi: 10.22092/ari.2021.356308.1817

Biochemical Evaluation of Carbonic Anhydrase and Some Antioxidant Markers in Patients with Diabetes Complications

Archives of Razi Institute
مقاله 24، دوره 77، شماره 1، فروردین و اردیبهشت 2022، صفحه 169-178    اصل مقاله (404.83 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2021.356308.1817
نویسندگان
A. H Sulaiman* ؛ Z. I Ghassan؛ T. N Omar
Chemistry Department, College of Science, University of Kirkuk, Kirkuk, Iraq
چکیده
Diabetes is a category of metabolic illnesses defined by a persistently high blood sugar level. This complication is caused by either the pancreas failing to create enough insulin or the body's cells failing to respond correctly to the insulin produced. Diabetes, if left untreated, can lead to a slew of health issues. Diabetic ketoacidosis, hyperosmolar hyperglycemia, and mortality are all examples of acute complications. There are numerous serious long-term consequences, including chronic renal disease, foot ulcers, as well as nerve and eye damage. This study aimed to extract carbonic anhydrase (CA) from human red blood cells and estimate the activity and specific activity of the enzyme and some biochemical parameters, including total protein, albumin, globulin, free amino acids, free amino acids/total protein (TP), thiol, thiol/TP, as well as carbonyl and carbonyl/TP levels in patients with diabetes complications, compared to the healthy subjects; moreover, it was attempted to investigate the correlation among the aforementioned variables. This study included 60 blood samples obtained from patients with diabetes complications and 40 healthy individuals as control. The results revealed a significant (P≤0.05) decrease in the TP levels, while the CA activity and specific activity were significantly (P≤0.05) increased. Moreover, there was a non-significant (P≥0.05) increase in the free amino levels; however, a significant (P≤0.05) increase was observed in albumin, free amino/TP, thiol, thiol/TP, as well as carbonyl and carbonyl/TP levels. On the other hand, a significant (P≤0.05) decrease was found in the levels of globulin and albumin/globulin ratio (AGR) in the patients, compared to the healthy subjects. The results also indicated a significant (P≤0.05) difference in all cases of diabetes mellitus (DM) complications for the measured parameters, except for the TP in DM nephropathy, albumin in cardiovascular disease, free amino in neuropathy and cardiovascular disease, and free amino/TP in retinopathy that showed a clear non-significant (P≥0.05) difference in the patients' groups, compared to the healthy subjects. The results of correlation indicated a significant (P≤0.05) positive correlation among free amino/TP, free amino/carbonyl, globulin/TP, and AGR/albumin. However, a significant negative correlation was noted between globulin/albumin and AGR/globulin. The results revealed that the protein oxidation markers and CA as antioxidant markers may play a role in monitoring diabetes complications.
کلیدواژه‌ها
Anti-oxidation markers؛ Carbonic anhydrase؛ Diabetes complications
سایر فایل های مرتبط با مقاله
مراجع
  1. Chow L, Seaquist ER. How Significant Is Severe Hypoglycemia in Older Adults With Diabetes? Diabetes Care. 2020;43(3):512-4.
  2. Cole JB, Florez JC. Genetics of diabetes mellitus and diabetes complications. Nat Rev Nephrol. 2020;16(7):377-90.
  3. Giacco F, Brownlee M. Oxidative stress and diabetic complications. Circ Res. 2010;107(9):1058-70.
  4. Ighodaro OM. Molecular pathways associated with oxidative stress in diabetes mellitus. Biomed Pharmacother. 2018;108:656-62.
  5. Brownlee M. The pathobiology of diabetic complications: a unifying mechanism. Diabetes. 2005;54(6):1615-25.
  6. Ismail IS. The Role of Carbonic Anhydrase in Hepatic Glucose Production. Curr Diabetes Rev. 2018;14(2):108-12.
  7. Occhipinti R, Boron WF. Role of Carbonic Anhydrases and Inhibitors in Acid-Base Physiology: Insights from Mathematical Modeling. Int J Mol Sci. 2019;20(15).
  8. Hainsworth R. Acid-Base Balance. Manchester University Press.1986.
  9. Qin J, Qin Y, Wu Y, Wei A, Luo M, Liao L, et al. Application of albumin/globulin ratio in elderly patients with acute exacerbation of chronic obstructive pulmonary disease. J Thorac Dis. 2018;10(8):4923-30.
  10. Wu PP, Hsieh YP, Kor CT, Chiu PF. Association between Albumin-Globulin Ratio and Mortality in Patients with Chronic Kidney Disease. J Clin Med. 2019;8(11).
  11. Murugan P, Pari L. Influence of tetrahydrocurcumin on hepatic and renal functional markers and protein levels in experimental type 2 diabetic rats. Basic Clin Pharmacol Toxicol. 2007;101(4):241-5.
  12. Yamakado M, Nagao K, Imaizumi A, Tani M, Toda A, Tanaka T, et al. Plasma Free Amino Acid Profiles Predict Four-Year Risk of Developing Diabetes, Metabolic Syndrome, Dyslipidemia, and Hypertension in Japanese Population. Sci Rep. 2015;5:11918.
  13. Oliveira PVS, Laurindo FRM. Implications of plasma thiol redox in disease. Clin Sci. 2018;132(12):1257-80.
  14. Kükürt A, Gelen V, Başer Ö, Deveci H, Karapehlivan M. Role in Oxidative Stress-Related Disorders. In: Atukeren P, editor. Accenting Lipid Peroxidation. IntechOpen. 2021.
  15. Winterbourn CC. Biological chemistry of superoxide radicals. ChemTexts. 2020;6(1):7.
  16. Menzel A, Samouda H, Dohet F, Loap S, Ellulu MS, Bohn T. Common and Novel Markers for Measuring Inflammation and Oxidative Stress Ex Vivo in Research and Clinical Practice-Which to Use Regarding Disease Outcomes? Antioxidants. 2021;10(3).
  17. Bora S, Shankarrao Adole P. Carbonyl stress in diabetics with acute coronary syndrome. Clin Chim Acta. 2021;520:78-86.
  18. Ghosh R, Mukherjee K, Maiti S, Bhattacharjee D, Chowdhuri S. Role of pleural fluid-serum protein carbonyl gradient in differentiating exudative and transudative effusions. Asian J Med Sci. 2020;11(6):46-52.
  19. Quattrini L, La Motta C. Aldose reductase inhibitors: 2013-present. Expert Opin Ther Pat. 2019;29(3):199-213.
  20. Pradeep AR, Ramchandraprasad MV, Bajaj P, Rao NS, Agarwal E. Protein carbonyl: An oxidative stress marker in gingival crevicular fluid in healthy, gingivitis, and chronic periodontitis subjects. Contemp Clin Dent. 2013;4(1):27-31.
  21. Tas M, Senturk E, Ekinci D, Demirdag R, Comakli V, Bayram M, et al. Comparison of blood carbonic anhydrase activity of athletes performing interval and continuous running exercise at high altitude. J Enzyme Inhib Med Chem. 2019;34(1):218-24.
  22. Ibrahim S, Amodu A, Ene-Ojo A, Ismaila U, Fakhruddeen M. Effect of hyperglycemia on erythrocyte carbonic anhydrase and lactic acid in type II diabetic subjects. J Diabetes Mellit. 2016;6(2):158-65.
  23. Verpoorte J, Mehta S, Edsall J. Esterase activities of human carbonic anhydrases B and C. J Biol Chem. 1967;242(18):4221-9.
  24. Parui R, Gambhir K, Cruz I, Hosten A. Erythrocyte carbonic anhydrase: a major intracellular enzyme to regulate cellular sodium metabolism in chronic renal failure patients with diabetes and hypertension. Biochem Int. 1992;26(5):809-20.
  25. Lowry O, Rosebrough N, Farr A, Randall R. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193:265-75.
  26. Zaia DAM, Barreto WJ, Santos NJ, Endo AS. Spectrophotometric method for the simultaneous determination of proteins and amino acids with p-benzoquinone. Analytica Chimica Acta. 1993;277(1):89-95.
  27. Hu M-L, Louie S, Cross C, Motchnik P, Halliwell B. Antioxidant protection against hypochlorous acid in human plasma. J Lab Clin Med. 1993;121(2):257-26.
  28. Riddles PW, Blakeley RL, Zerner B. Ellman's reagent: 5,5′-dithiobis(2-nitrobenzoic acid)—a reexamination. Anal Biochem. 1979;94(1):75-81.
  29. Levine RL, Garland D, Oliver CN, Amici A, Climent I, Lenz A-G, et al. Determination of carbonyl content in oxidatively modified proteins. Methods in Enzymology. 186: Academic Press; 1990. p. 464-78.
  30. Muller K, Brunnberg L. Determination of plasma albumin concentration in healthy and diseased turtles: a comparison of protein electrophoresis and the bromcresol green dye-binding method. Vet Clin Pathol. 2010;39(1):79-82.
  31. Jaganjac M, Tirosh O, Cohen G, Sasson S, Zarkovic N. Reactive aldehydes--second messengers of free radicals in diabetes mellitus. Free Radic Res. 2013;47 Suppl 1:39-48.
  32. Bassey I, Udo E, Adesite S. Effect of crude aqueous leaves extract of BryophyllumPinnatum on antioxidant status, blood glucose, lipid profile, liver and renal function indices in albino rats. Glob J Pure Appl Sci. 2021;27(2):231-41.
  33. Hussein S, Zainal I. Human carbonic anhydrase: Purification and characterization study in thalassemia major patients compared to healthy subjects. Med J Babylon. 2018;15(4):349.
  34. Al-Muhtaseb SI. Serum and saliva protein levels in females with breast cancer. Oncol Lett. 2014;8(6):2752-6.
  35. Yassin MA, Soliman AT, De Sanctis V, Hussein RM, Al-Okka R, Kassem N, et al. Jadenu((R)) Substituting Exjade((R)) in Iron Overloaded beta-Thalassemia Major (BTM) Patients: A Preliminary Report of the Effects on the Tolerability, Serum Ferritin Level, Liver Iron Concentration and Biochemical Profiles. Mediterr J Hematol Infect Dis. 2018;10(1):2018064.
  36. Toiyama Y, Yasuda H, Ohi M, Yoshiyama S, Araki T, Tanaka K, et al. Clinical impact of preoperative albumin to globulin ratio in gastric cancer patients with curative intent. Am J Surg. 2017;213(1):120-6.
  37. Sayed N, Huang Y, Nguyen K, Krejciova-Rajaniemi Z, Grawe AP, Gao T, et al. Author Correction: An inflammatory aging clock (iAge) based on deep learning tracks multimorbidity, immunosenescence, frailty and cardiovascular aging. Nature Aging. 2021;1(8):748.
  38. Trifunović-Macedoljan J, Pantelić N, Damjanović A ea. LC/DAD determination of biogenic amines in serum of patients with diabetes mellitus, chronic urticaria or Hashimoto's thyroiditis. J Serbian Chem Soc. 2016;81(5):487-98.
  39. Saleem T, Dahpy M, Ezzat G, Abdelrahman G, Abdel-Aziz E, Farghaly R. The Profile of Plasma Free Amino Acids in Type 2 Diabetes Mellitus with Insulin Resistance: Association with Microalbuminuria and Macroalbuminuria. Appl Biochem Biotechnol. 2019;188(3):854-67.
  40. Mihalik SJ, Michaliszyn SF, de las Heras J, Bacha F, Lee S, Chace DH, et al. Metabolomic profiling of fatty acid and amino acid metabolism in youth with obesity and type 2 diabetes: evidence for enhanced mitochondrial oxidation. Diabetes Care. 2012;35(3):605-11.
  41. Ates I, Kaplan M, Yuksel M, Mese D, Alisik M, Erel O, et al. Determination of thiol/disulphide homeostasis in type 1 diabetes mellitus and the factors associated with thiol oxidation. Endocrine. 2016;51(1):47-51.
  42. Sener S, Akbas A, Kilinc F, Baran P, Erel O, Aktas A. Thiol/disulfide homeostasis as a marker of oxidative stress in rosacea: a controlled spectrophotometric study. Cutan Ocul Toxicol. 2019;38(1):55-8.
  43. Ergin Tuncay M, Erkilic A, Gunes A, Nural C, Erel O. A remarkable point for evaluating the severity of burns: Thiol-disulfide profile. Burns. 2020;46(4):882-7.
  44. Darmaun D, Smith SD, Sweeten S, Sager BK, Welch S, Mauras N. Evidence for accelerated rates of glutathione utilization and glutathione depletion in adolescents with poorly controlled type 1 diabetes. Diabetes. 2005;54(1):190-6.
  45. Matsumoto N, Omagari D, Ushikoshi-Nakayama R, Yamazaki T, Inoue H, Saito I. Hyperglycemia Induces Generation of Reactive Oxygen Species and Accelerates Apoptotic Cell Death in Salivary Gland Cells. Pathobiology. 2021;88(3):234-41.
  46. Abd I, Zainal I. Assessment of biochemical parameters and study its correlation in ß-Thalassemia major patients and healthy controls in Kirkuk City, Iraq. Med J Babylon. 2020;17(2):172.
  47. Mateen S, Moin S, Khan AQ, Zafar A, Fatima N. Increased Reactive Oxygen Species Formation and Oxidative Stress in Rheumatoid Arthritis. PLoS One. 2016;11(4):e0152925.
  48. Odetti P, Garibaldi S, Noberasco G, Aragno I, Valentini S, Traverso N, et al. Levels of carbonyl groups in plasma proteins of type 2 diabetes mellitus subjects. Acta Diabetol. 1999;36(4):179-83.
  49. Noah K, Hmood F, Zainal I. Estimation and isolation of ceruloplasmin and some biochemical indicators in diabetes mellitus type II patients compared to healthy controls in Kirkuk Province, Iraq. Med J Babylon. 2020;17(1):49.
  1. Dalle-Donne I, Rossi R, Giustarini D, Milzani A, Colombo R. Protein carbonyl groups as biomarkers of oxidative stress. Clinica Chimica Acta. 2003;329(1):23-38.
آمار
تعداد مشاهده مقاله: 1,782
تعداد دریافت فایل اصل مقاله: 2,672


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب