| Molds have been used as micro-biofactories for biomanufacturing of metal oxide nanoparticles (MetNps) since they are effortless, immaculate, safe, non-poisonous, vital-biocompatible, and environmentally acceptable. The present study aimed to explore the bioindustry, mold screening protocol, and characterization of zinc oxide nanoparticles (ZnONPs) using a diverse filamentous Green mold (FiGM) isolated from spoiled citrus fruits. Eight filamentous Penicillium digitatum mold strains had been obtained and subjected to investigate the capability of ZnONPs biosynthesis by fungal extracellular free-cell filtrate. P. digitatum (P-digB3) obtained the peak of ZnONps (379 nm) detected by the UV-visible spectrophotometry and was found as a significantly optimum strain in the highest quantity (mean±SD: 0.0138±0.001 gm/100 ml) and the smallest average NPs size. The ZnONPs were characterized by UV-visible scanning spectrophotometry, Atomic Force Microscopy, X-RD, Scanning Electron Microscopy (SEM), and Transmission Electron Microscopy (TEM). The final average size of ZnONPs was obtained at 65.46 nm with diversified shapes and dimensions. The present study concluded the high capabilities of fungi (FiGMs) as eco-friendly and cheap bio-nano factories to manufacture ZnONPs with great nano-level average size, which may consider new boost sources for use in many nano-sectors and applications. |
- Yusof HM, Mohamad R, Zaidan UH. Microbial synthesis of zinc oxide nanoparticles and their potential application as an antimicrobial agent and a feed supplement in animal industry: a review. J Anim Sci Biotechnol. 2019;10(1):1-22.
- Li H, Yan S, Deng C, Dou S, Ren X, editors. Synthesis and characterization of Ag nanoflowers with different morphologies. IOP Conference Series: Materials Science and Engineering; 2021: IOP Publishing.
- Kareem S, Jarullah BA. Improvement of Newcastle disease virus vaccine by using gold nanoparticles and some natural food additives. Univ Thi-Qar J Sci. 2017;6(2):59-64.
- da Silva BL, Abuçafy MP, Manaia EB, Junior JAO, Chiari-Andréo BG, Pietro RCR, et al. Relationship between structure and antimicrobial activity of zinc oxide nanoparticles: An overview. Int J Nanomedicine. 2019;14:9395.
- Kalpana V, Kataru BAS, Sravani N, Vigneshwari T, Panneerselvam A, Rajeswari VD. Biosynthesis of zinc oxide nanoparticles using culture filtrates of Aspergillus niger: Antimicrobial textiles and dye degradation studies. OpenNano. 2018;3:48-55.
- Hefny ME, El-Zamek FI, El-Fattah A, Mahgoub S. Biosynthesis of Zinc Nanoparticles Using Culture Filtrates of Aspergillus, Fusarium and Penicillium Fungal Species and Their Antibacterial Properties against Gram-Positive and Gram-Negative Bacteria. Zagazig J Agric Res. 2019;46(6):2009-21.
- Issa MA, Al-Sheikhly AA, Hamid MK, Nader M. Novel rapid green fabrication of ZnO nps using mycofiltrate by local fungus Aspergillus Parasiticus Ap4. Biosci Res. 2018;15(3):2159-70.
- Taha ZK, Howar SN, Sulaiman GM. Isolation and identification of Penicillium italicum from Iraqi citrus lemon fruits and its ability manufacture of silver nanoparticles and their antibacterial and antifungal activity. Res J Pharm Technol. 2019;12(3):1320-6.
- Elfita E, Muharni M, Yohandini H, Fadhillah F, editors. Antioxidant activity of endophytic fungi isolated from the stem bark of Swietenia mahagoni (L.) Jacq. IOP Conference Series: Materials Science and Engineering; 2021: IOP Publishing.
- Sri V, Rajagopal K. Isolation and Identification of Thermo Tolerant Endophytic Fungi from Melia dubia and Synthesis of Zinc Nano ParticlesJ. Nanosci Nanotechnol. 2016;7(2):99-112.
- Pitt JI, Hocking AD. Fungi and food spoilage: Springer; 2009.
- Visagie C, Houbraken J, Frisvad JC, Hong S-B, Klaassen C, Perrone G, et al. Identification and nomenclature of the genus Penicillium. Stud Mycol. 2014;78:343-71.
- Ottoni CA, Simões MF, Fernandes S, Dos Santos JG, Da Silva ES, de Souza RFB, et al. Screening of filamentous fungi for antimicrobial silver nanoparticles synthesis. AMB Express. 2017;7(1):1-10.
- Rajan A, Cherian E, Baskar G. Biosynthesis of zinc oxide nanoparticles using Aspergillus fumigatus JCF and its antibacterial activity. Int J Mod Sci Technol. 2016;1:52-7.
- Baskar G, Chandhuru J, Fahad KS, Praveen A. Mycological synthesis, characterization and antifungal activity of zinc oxide nanoparticles. Asian J Pharm Technol. 2013;3(4):142-6.
- Mahmood S, Mandal UK. Morphological characterization of lipid structured nanoparticles by atomic force microscopy while minimizing the formation of failed artefacts. Curr Nanosci. 2017;2(1):24-32.
- Bayroodi E, Jalal R. Modulation of antibiotic resistance in Pseudomonas aeruginosa by ZnO nanoparticles. Iran J Microbiol. 2016;8(2):85.
- Chauhan R, Reddy A, Abraham J. Biosynthesis of silver and zinc oxide nanoparticles using Pichia fermentans JA2 and their antimicrobial property. Appl Nanosci. 2015;5(1):63-71.
- Singh P, Kim Y-J, Zhang D, Yang D-C. Biological synthesis of nanoparticles from plants and microorganisms. Trends Biotechnol. 2016;34(7):588-99.
- Khandel P, Shahi SK. Microbes mediated synthesis of metal nanoparticles: current status and future prospects. Int J Nanomater Biostruct. 2016;6(1):1-24.
- Mashrai A, Khanam H, Aljawfi RN. Biological synthesis of ZnO nanoparticles using C. albicans and studying their catalytic performance in the synthesis of steroidal pyrazolines. Arab J Chem. 2017;10:S1530-S6.
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