اخبار و اعلانات

 آمار

تعداد نشریات285
تعداد نشریات سازمان83
تعداد شماره‌ها3,115
تعداد مقالات34,688
تعداد مشاهده مقاله49,905,453
تعداد دریافت فایل اصل مقاله80,224,285
Hammadi Jasim, N, Adel Kareem, D, Majeed AL Ali, M. F, Abbas, B. A. (1400). Effect of Long-Term Treatment with Dexamethasone on the Liver and Kidney Histopathology, as well as Blood Biochemistry in Male Rabbits (Lepus Cuniculus). سامانه مدیریت نشریات علمی, 77(1), 333-343. doi: 10.22092/ari.2021.356468.1847
N Hammadi Jasim; D Adel Kareem; M. F Majeed AL Ali; B. A Abbas. "Effect of Long-Term Treatment with Dexamethasone on the Liver and Kidney Histopathology, as well as Blood Biochemistry in Male Rabbits (Lepus Cuniculus)". سامانه مدیریت نشریات علمی, 77, 1, 1400, 333-343. doi: 10.22092/ari.2021.356468.1847
Hammadi Jasim, N, Adel Kareem, D, Majeed AL Ali, M. F, Abbas, B. A. (1400). 'Effect of Long-Term Treatment with Dexamethasone on the Liver and Kidney Histopathology, as well as Blood Biochemistry in Male Rabbits (Lepus Cuniculus)', سامانه مدیریت نشریات علمی, 77(1), pp. 333-343. doi: 10.22092/ari.2021.356468.1847
Hammadi Jasim, N, Adel Kareem, D, Majeed AL Ali, M. F, Abbas, B. A. Effect of Long-Term Treatment with Dexamethasone on the Liver and Kidney Histopathology, as well as Blood Biochemistry in Male Rabbits (Lepus Cuniculus). سامانه مدیریت نشریات علمی, 1400; 77(1): 333-343. doi: 10.22092/ari.2021.356468.1847

Effect of Long-Term Treatment with Dexamethasone on the Liver and Kidney Histopathology, as well as Blood Biochemistry in Male Rabbits (Lepus Cuniculus)

Archives of Razi Institute
مقاله 43، دوره 77، شماره 1، فروردین و اردیبهشت 2022، صفحه 333-343    اصل مقاله (1.25 M)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2021.356468.1847
نویسندگان
N Hammadi Jasim1؛ D Adel Kareem2؛ M. F Majeed AL Ali* 2؛ B. A Abbas3
1Department of Physiology and Pharmacology and Chemistry, College of Veterinary Medicine, University of Basrah, Basrah, Iraq
2Department of Anatomy and Histology, College of Veterinary Medicine, University of Basrah, Basrah, Iraq
3Department of Microbiology and Parasitology, College of Veterinary Medicine, University of Basrah, Basrah, Iraq
چکیده
Glucocorticoids have a wide range of pharmacological activities. Generally speaking, the steroid drugs, such as dexamethasone (DEX) can have severe side effects on the histology of different organs. In fact, glucocorticoids have been known as powerful medicines which can cure inflammation and work with the immune system to treat a wide range of health problems. Therefore, this study aimed to investigate the effects of DEX on the histological changes of the liver and kidney, as well as blood biochemical parameters. In total, 13 specific pathogen-free male Lepus cuniculus rabbits aged 8-10 months old, with a mean weight of 1.12±0.13 kg were randomly divided into three groups. Group I (n=3) did not receive DEX, and they only received saline solution as a placebo (control). In Group II (n=5), the animals received 0.25 mg DEX/kg body weight/day for a period of 56 days, and the animals in Group III (n=5) received 0.5 mg DEX/kg body weight/day for 56 days. Blood was aspirated from the rabbit's marginal ear vein. All blood samples were centrifuged at 3000×g for 10 min to separate serum samples. Blood lipids and trace elements (zinc, copper, calcium, and iron) were measured. The microscopical analyses of the liver and kidney tissues were performed through the observation of the histological changes in the tissues. The results showed a significant (P≤0.05) decrease in the body and organ weight, as well as serum concentrations for the trace elements. On the other hand, lipid profile showed a significant increase (P≤0.05) in cholesterol, triglycerides, and low-density lipoprotein. However, a significant decrease was recorded in high-density lipoprotein in both treated groups with DEX, compared to the control group. The results of the histological evaluation showed some degrees of degeneration, necrosis, cell vacuolation, and lymphocyte infiltration in the kidney and liver tissues in the treatment groups.
کلیدواژه‌ها
Dexamethasone؛ Essential Mineral؛ Histology؛ Lipid Profile
سایر فایل های مرتبط با مقاله
مراجع
  1. Baxter JD, Forsham PH. Tissue effects of glucocorticoids. Am J Med. 1972;53(5):573-89.
  2. Severino C, Brizzi P, Solinas A, Secchi G, Maioli M, Tonolo G. Low-dose dexamethasone in the rat: a model to study insulin resistance. Am J Physiol Endocrinol Metab. 2002;283(2):E367-73.
  3. Ganong WF. Review of Medical Physiology. 21st ed. New York, U.S.A.: McGraw Hill Company; 2003.
  4. Eid Y, Ebeid T, Moawad M, El-Habbak M. Reduction EMEA (European Medicines Agency) (2004) Committee for medicinal products for veterinary use dexamethasone. Vet Med Inspect. 2008.
  5. Williams TJ, Yarwood H. Effect of glucocorticosteroids on microvascular permeability. Am Rev Respir Dis. 1990;20(2):28-40.
  6. Yarwood H, Nourshargh S, Brain S, Williams TJ. Effect of dexamethasone on neutrophil accumulation and oedema formation in rabbit skin: an investigation of site of action. Br J Pharmacol. 1993;108(4):959-66.
  7. Luna LG. Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology: McGraw-Hill; 1968.
  8. Bennett P, Brown M. Clinical Pharmacology. 10th ed. London: Churchill Livingstone. Elsevier; 2008.
  9. Freake HC, Govoni KE, Guda K, Huang C, Zinn SA. Actions and interactions of thyroid hormone and zinc status in growing rats. J Nutr. 2001;131(4):1135-41.
  10. Franco-Colin M, Villanueva I, Pinon M, Racotta R. The effects of sympathectomy and dexamethasone in rats ingesting sucrose. Int J Biol Sci. 2006;2(1):17-22.
  11. Smith C, Erasmus PJ, Myburgh KH. Endocrine and immune effects of dexamethasone in unilateral total knee replacement. J Int Med Res. 2006;34(6):603-11.
  12. Weiler HA, Wang Z, Atkinson SA. Dexamethasone treatment impairs calcium regulation and reduces bone mineralization in infant pigs. Am J Clin Nutr. 1995;61(4):805-11.
  13. He J, Varma A, Weissfeld LA, Devaskar SU. Postnatal glucocorticoid exposure alters the adult phenotype. Am J Physiol Regul Integr Comp Physiol. 2004;287(1):198-208.
  14. Plonné D, Schulze H-P, Kahlert U, Meltke K, Seidolt H, Bennett AJ, et al. Postnatal development of hepatocellular apolipoprotein B assembly and secretion in the rat. J Lipid Res. 2001;42(11):1865-78.
  15. Bruder ED, Lee PC, Raff H. Metabolic consequences of hypoxia from birth and dexamethasone treatment in the neonatal rat: comprehensive hepatic lipid and fatty acid profiling. Endocrinology. 2004;145(11):5364-72.
  16. Nicod N, Giusti V, Besse C, Tappy L. Metabolic adaptations to dexamethasone-induced insulin resistance in healthy volunteers. Obes Res. 2003;11(5):625-31.
  17. Arab Dolatabadi A, Mahboubi M. A study of the influence of dexamethasone on lipid profile and enzyme lactate dehydrogenase. J Med Life. 2015;8(Spec Iss 3):72-6.
  18. Wang CN, McLeod RS, Yao Z, Brindley DN. Effects of dexamethasone on the synthesis, degradation, and secretion of apolipoprotein B in cultured rat hepatocytes. Arterioscler Thromb Vasc Biol. 1995;15(9):1481-91.
  19. Singh C, Singha SPS. Effect of Dexamethasone Stress on Concentrations of Zinc in Blood Plasma and in Sub-Cellular Fractions of Various Tissues of Neonatal Buffalo Calves. Asian-Australas J Anim Sci. 2002;15(7):1022-5.
  20. Yunice AA, Czerwinski AW, Lindeman RD. Influence of synthetic corticosteroids on plasma zinc and copper levels in humans. Am J Med Sci. 1981;282(2):68-74.
  21. Lukert BP, Raisz LG. Glucocorticoid-induced osteoporosis: pathogenesis and management. Ann Intern Med. 1990;112(5):352-64.
  22. Kernan KF, Carcillo JA. Hyperferritinemia and inflammation. Int Immunol. 2017;29(9):401-9.
  1. Jeklova E, Leva L, Jaglic Z, Faldyna M. Dexamethasone-induced immunosuppression: a rabbit model. Vet Immunol Immunopathol. 2008;122(3-4):231-40.
  2. Martins JP, Monteiro JC, Paixao AD. Renal function in adult rats subjected to prenatal dexamethasone. Clin Exp Pharmacol Physiol. 2003;30(1-2):32-7.
  3. Júnior P, Teixeira A, Teixeira V, Moraes E, Araújo A, Maia C. Morphological Analysis of Neonates of Rats Treated with Dexamethasone in the Initial Phase of Pregnancy. Int J Morphol. 2008;26.
  4. Giannopoulos G, Hassan Z, Solomon S. Glucocorticoid Receptors in Fetal and Adult Rabbit Tissues. J Biol Chem. 1974;249(8):2424-7.
  5. Roy MJ, Walsh TJ. Histopathologic and immunohistochemical changes in gut-associated lymphoid tissues after treatment of rabbits with dexamethasone. Lab Invest. 1992;66(4):437-43.
  6. Rooman R, Koster G, Bloemen R, Gresnigt R, van Buul-Offers SC. The effect of dexamethasone on body and organ growth of normal and IGF-II-transgenic mice. J Endocrinol. 1999;163(3):543-52.
 

آمار
تعداد مشاهده مقاله: 1,965
تعداد دریافت فایل اصل مقاله: 2,862


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب