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بررسی خصوصیات فیتوشیمیایی، آنتی‌اکسیدانی، ضد باکتریایی و ضد التهابی Ducrosia anethifolia (DC.) Boiss. در مراحل مختلف فنولوژی | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| دوره 37، شماره 6 - شماره پیاپی 110، بهمن و اسفند 1400، صفحه 971-988 اصل مقاله (851.6 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2021.355808.3056 | ||
| نویسنده | ||
مجید شریفی راد  
| ||
| استادیار، گروه مرتع و آبخیزداری، دانشگاه زابل، زابل، ایران | ||
| چکیده | ||
| مشگک (Ducrosia anethifolia (DC.) Boiss.) یکی از گیاهان دارویی متعلق به خانواده چتریان است که استفاده از آن در درمان اختلالات عصبی توصیه شده است. هدف مطالعه حاضر بررسی تغییرات محتوای ترکیب‌های فیتوشیمیایی و فعالیت آنتی‌اکسیدانی، ضد باکتریایی و ضد التهابی عصاره اتانولی گونه D. anethifolia در مراحل مختلف فنولوژی (رویشی، گلدهی و بذردهی) بود. نمونه‌برداری از سرشاخه‌های هوایی گیاه در هر مرحله به‌صورت تصادفی انجام گرفت. محتوای فنل و فلاونوئید کل عصاره اتانولی با استفاده از روش اسپکتروفتومتری و فعالیت آنتی‌اکسیدانی با استفاده از روش به دام‌اندازی رادیکال آزاد DPPH تعیین شد. فعالیت ضد باکتریایی عصاره گیاه با استفاده از روش انتشار دیسک بر روی باکتری‌های گرم مثبت (Staphylococcus aureus و Bacillus subtilis) و گرم منفی (Escherichia coli و Pseudomonas aeruginosa) بررسی گردید. همچنین، فعالیت ضد التهابی عصاره گیاه با استفاده از روش پایداری غشای گلبول قرمز ارزیابی شد. نتایج نشان داد که بین مراحل مختلف فنولوژی از نظر میزان فنل و فلاونوئید کل و فعالیت آنتی‌اکسیدانی، ضد باکتریایی و ضد التهابی تفاوت معنی‌داری وجود دارد (0.05>P). بیشترین میزان فنل و فلاونوئید کل (به‌ترتیب 1.7 ± 148 میلی‌گرم معادل اسید گالیک بر گرم وزن خشک و 1.5 ± 97 میلی‌گرم معادل کوئرستین بر گرم وزن خشک) و فعالیت آنتی‌اکسیدانی، ضد باکتریایی و ضد التهابی در مرحله گلدهی به‌دست آمد. همچنین نتایج نشان داد که عصاره گیاهی مورد مطالعه اثر بازدارندگی بیشتری بر باکتری‌های گرم مثبت در مقایسه با باکتری‌های گرم منفی دارد. نتایج بیانگر آن است که عصاره اتانولی گونه D. anethifolia می‌تواند به‌عنوان یک آنتی‌اکسیدان، ضد باکتری و ضد التهاب امیدوار کننده در درمان بسیاری از بیماری‌ها استفاده شود. همچنین، مرحله گلدهی بهترین زمان برداشت جهت بهره‌برداری دارویی از این گونه معرفی می‌شود. | ||
| کلیدواژه‌ها | ||
| فعالیت آنتی‌اکسیدانی؛ فعالیت ضد باکتریایی؛ فعالیت ضد التهابی؛ Ducrosia anethifolia (DC.) Boiss؛ فنولوژی | ||
| عنوان مقاله [English] | ||
| Evaluation of phytochemical, antioxidant, antibacterial and anti-inflammatory properties of Moshgak (Ducrosia anethifolia (DC.) Boiss.) at different phenological stages | ||
| نویسندگان [English] | ||
| M. Sharifi-Rad | ||
| Department of Range and Watershed Management, Faculty of Water and Soil, University of Zabol, Zabol, Iran | ||
| چکیده [English] | ||
| Ducrosia anethifolia (DC.) Boiss. is one of the medicinal plants belonging to the fam. apiaceae that has been recommended as a treatment for the neurological disorders. The present study was aimed at investigating the changes in the phytochemicals content and the antioxidant, antibacterial, and anti-inflammatory properties of D. anethifolia ethanolic extract at the different phenological stages (vegetative, flowering, and seeding). The plant shoots were collected at each stage randomly. The total phenols and flavonoids contents of the ethanolic extracts were measured using the spectrophotometry method and the antioxidant activity was determined by the DPPH free radical scavenging method. The disk diffusion method was used to investigate the antibacterial activity of the plant extract against Gram-positive (Staphylococcus aureus and Bacillus subtilis) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria. Also, the anti-inflammatory activity of the plant extract was assessed using the human red blood cell stabilization method. The results showed that there was a significant difference between the different phenological stages in terms of the total phenols and flavonoids contents and the antioxidant, antibacterial, and anti-inflammatory properties (P <0.05). The highest amount of total phenols and flavonoids (148 ± 1.7 mg Gallic acid equivalents (GAE)/g dry weight and 97 ± 1.5 mg Quercetin equivalents (QE)/g dry weight, respectively) and antioxidant, antibacterial, and anti-inflammatory activities was observed at the flowering stage. The results also showed that the plant extract studied had a greater inhibitory effect on the Gram-positive bacteria than the Gram-negative ones. The results indicated that the ethanolic extract of D. anethifolia could be used as a promising agent with the antioxidant, antibacterial, and anti-inflammatory properties to treat many diseases. Also, the flowering stage could be introduced as the best harvest time for the medicinal use of this species. | ||
| کلیدواژه‌ها [English] | ||
| Antioxidant activity, antibacterial activity, anti-inflammatory activity, Ducrosia anethifolia (DC.) Boiss, phenology | ||
| مراجع | ||
|
- Alizadeh, A., 2015. Essential oil composition, phenolic content, antioxidant, and antimicrobial activity of cultivated Satureja rechingeri Jamzad at different phenological stages. Zeitschrift für Naturforschung C, 70: 51-58. - Altemimi, A., Lakhssassi, N., Baharlouei, A., Watson, D.G. and Lightfoot, D.A., 2017. Phytochemicals: Extraction, isolation, and identification of bioactive compounds from plant extracts. Plants, 6(4): 42. - Ambriz-Pérez, D.L., Leyva-López, N., Gutierrez-Grijalva, E.P. and Heredia, J.B., 2016. Phenolic compounds: Natural alternative in inflammation treatment. a Review. Cogent Food & Agriculture, 2(1): 162-171. - Amjad, L., Mohammadi Kamalabadi, M. and Mohammadi Sichani, M., 2011. Antibacterial activity of methanol extract of Achillea wilhe lmsii C. Koch flower and leaf. Qom University of Medical Sciences Journal, 5(3): 50-56. - Arumugam, P., Ramamurthy, P. and Ramesh, A., 2010. Antioxidant and cytotoxic activities of lipophilic and hydrophilic fractions of Mentha spicata L. (Lamiaceae). International Journal of Food Properties, 13(1): 23-31. - Bag, A., Kumar Bhattacharyya, S., Kumar Pal, N. and Ranjan Chattopadhyay, R., 2013. Anti-inflammatory, anti-lipid peroxidative, antioxidant and membrane stabilizing activities of hydroalcoholic extract of Terminalia chebula fruits. Pharmaceutical Biology, 51: 1515-1520. - Bai, N., He, K., Roller, M., Lai, C.S., Shao, X., Pan, M.H. and Ho, C.T., 2010. Flavonoids and phenolic compounds from Rosmarinus officinalis. Journal of Agricultural and Food Chemistry, 58(9): 5363-5367. - Beckman, C.H., 2000. Phenolic storing cells: keys to programmed cell death and periderm formation in wilt disease resistance and in general defense responses in plants? Physiological and Molecular Plant Pathology, 57: 101-110. - Chang, C., Yang, M., Wen, H. and Chern, J., 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis, 10: 178-182. - Cheung, S. and Tai, J., 2007. Anti-proliferative and antioxidant properties of rosemary Rosmarinus officinalis. Oncology Reports, 17: 1525-1531. - Chouaieb, H., Ayadi, I., Zouari, S., Fakhfakh, N., Zaidi, S. and Zouari, N., 2012. Effect of phenological stage and geographical location on antioxidant activities of tunisian horehound: Marrubium vulgare L. (Lamiaceae). Journal of Biologically Active Products from Nature, 2(4): 232-238. - Das, K., Tiwari, R.K.S. and Shrivastava, D.K., 2010. Techniques for evaluation of medicinal plant products as antimicrobial agent: Current methods and future trends. Journal of Medicinal Plants Research, 4(2): 104-111. - de la Lastra, C.A. and Villegas, I., 2005. Resveratrol as an anti-inflammatory and anti-aging agent: Mechanisms and clinical implications. Molecular Nutrition & Food Research, 49(5): 405-430. - Ebrahimi, A., Khayami, M. and Nejati, V., 2012. Comparison of Antimicrobial effect of different parts of Quercus persica against Escherichia coli O157:H7. Horizon of Medical Sciences, 17(4):11-17. - Górniak, I., Bartoszewski, R. and Króliczewski, J., 2019. Comprehensive review of antimicrobial activities of plant flavonoids. Phytochemistry Reviews, 18(1): 241-272. - Haghi, G., Safaei, A. and Safari, J., 2004. Extraction and determination of the main components of the essential oil of Ducrosia anethifolia by GC and GC/MS. Iranian Journal of Pharmaceutical Research, 3(2): 90-91. - Heimer, L. and Alheid, G.F., 1991. Piecing together the puzzle of basal forebrain anatomy. Advances in Experimental Medicine and Biology, 295: 1-42. - Hoodgar, F., Nasri, S. and Amin, G., 2011. Investigation of antinociceptive and anti-inflammatory effects of hydro-alcoholic extract of Securigera securidaca. Horizon of Medical Sciences, 17(1): 12-19. - Jakovljević, D.Z., Stanković, S.M. and Topuzović, D.M., 2013. Seasonal variability of Chelidonium majus L. secondary metabolites content and antioxidant activity. EXCLI Journal, 12: 260-268. - Jardim, M.C., 2005. Role of glutamate ionotropic and benzodiazepine receptors in the ventromedial hypothalamic nucleus on anxiety. Pharmacology Biochemistry and Behavior, 82(1): 182-189. - Joshi, B. and Lekhak, S., 2009. Antibacterial property of different medicinal plants. Journal of Science Engineering and Technology, 5: 143-150. - Khamis, A.D.S. and Chai, L.C., 2021. Chemical and antimicrobial analyses of Juniperus chinensis and Juniperus seravschanica essential oils and comparison with their methanolic crude extracts. International Journal of Analytical Chemistry, 2021: 1-8. - Khodabande, Z., Jafarian, V. and Sariri, R., 2017. Antioxidant activity of Chelidonium majus extract at phenological stages. Applied Biological Chemistry, 60(5): 497-503. - Mckeegan, K.S., Borges Walmsley, M.I. and Walmsley, A.R., 2002. Microbial and viral drug resistance mechanisms. Trends in Microbiology, 10(10): S8-S14. - Meda, A., Lamien, C.E., Romito, M., Millogo, J. and Nacoulma, O.G., 2005. Determination of the total phenolic, flavonoid and pralin contents in Burkina Fasan honey, as well as their scavenging activity. Food Chemistry, 91: 571-577. - Moghaddam, M., Khaleghi Miran, S.N. and Mehdizadeh, L., 2018. Total phenolic content and antioxidant activity of Fumaria vaillantii extract at three phenological stages assayed by various methods. International Journal of Horticultural Science and Technology, 5(1): 93-102. - Mottaghipisheh, J., Maghsoudlou, M.T., Valizadeh, J. and Arjomandi, R., 2014. Antioxidant activity and chemical composition of the essential oil of Ducrosia anethifolia (DC.) Boiss. from Neyriz. Journal of Medicinal plants and By-Product, 3(2): 215-218. - Mottaghipisheh, J., Nové, M., Spengler, G., Kúsz, N., Hohmann, J. and Csupor, D., 2018. Antiproliferative and cytotoxic activities of furocoumarins of Ducrosia anethifolia. Pharmaceutical Biology, 56: 658-664. - MyungJin, C., Yohannes, S.B., Damte, D., Reza, M. and TaeHan, K., 2012. The in vitro antibacterial activity of enrofloxacin trimethoprim combination against five bacterial species. Pakistan Veterinary Journal, 32(3): 363-366. - Nisar, B., Sultan, A. and Rubab, S.L., 2018. Comparison of medicinally important natural products versus synthetic drugs-a short commentary. Natural Products Chemistry and Research, 6(2): 308. - Nunes, C.D.R., Barreto Arantes, M., Menezes de Faria Pereira, S., Leandro da Cruz, L., de Souza Passos, M., Pereira de Moraes, L., Vieira, I.J.C. and de Oliveira, D.E., 2020. Plants as sources of anti-inflammatory agents. Molecules, 25(16): 3726. - Rankovič, B., Rankovic, D. and Maric, D., 2010. Antioxidant and antimicrobial activity of some lichen species. Microbiology, 79(6): 809-815. - Rojas, J.J., Ochoa, V.J., Ocampo, S.A. and Muñoz, J.F., 2006. Screening for antimicrobial activity of ten medicinal plants used in Colombian folkloric medicine: A possible alternative in the treatment of non-nosocomial infections. BMC Complementary Medicine and Therapies, 6(1): 1-6. - Shahid, M., Shahzad, A., Sobia, F., Sahai, A., Tripathi, T., Singh, A. and Khan, H.M., 2009. Plant natural products as a potential source for antibacterial agents: recent trends. Anti-Infective Agents in Medicinal Chemistry, 8(3): 211-225. - Shailesh, G., Seema, K. and Dwivedi, S., 2011. In-vitro anti-inflammatory activity of Sarcostemma acidum Wight. & Arn. Indian herb by Human red blood cell membrane stabilization method. International Journal of Pharmacy Teaching & Practices, 2: 184-188. - Sharifi-Rad, M., Epifano, F., Fiorito, S. and Álvarez-Suarez, J.M., 2020. Phytochemical analysis and biological investigation of Nepeta juncea Benth. different extracts. Plants, 9(5): 646. - Simpson, T.S., Savage, G.P., Sherlock, R. and Vanhanen, L.P., 2009. Oxalate content of silver beet leaves (Beta vulgaris var. cicla) at different stages of maturation and the effect of cooking with different milk sources. Journal of Agricultural and Food Chemistry, 57(22): 10804-10808. - Smith, H., 1973. Regulatory mechanisms in the photocontrol of flavonoid biosynthesis: 303-320. In: Milborrow, B.V., (Ed.). Biosynthesis and Its Control in Plants. New York, Academic Press, 364p. - Stavri, M., Mathew, K.T., Bucar, F. and Gibbons, S., 2003. Pangelin, an antimycobacterial coumarin from Ducrosia anethifolia. Planta Medica, 69(10): 956-959. - Toyang, N.J. and Verpoorte, R., 2013. A review of the medicinal potentials of plants of the genus Vernonia (Asteraceae). Journal of Ethnopharmacology, 146(3): 681-723. - Tsuruga, M., Nakajima, H. and Magae, J., 2007. Immunosuppressive activity of 4-O-methylascochlorin. The Journal of Antibiotics, 60(1): 20-26. - Vane, J.R. and Botting, R.M., 1995. New insights into the mode of action of anti-inflammatory drugs. Inflammation Research, 44(1): 1-10. - Wegiera, M., Grabarczyk, P., Baraniak, B. and Smolarz, H.D., 2011. Antiradical properties of extracts from roots, leaves and fruits of six Rumex L. species. Acta Biologica Cracoviensia Series Botanica, 53(1): 125-131. - Weli, A.M., Al-Salmi, S., Al Hoqani, H. and Hossain, M.A., 2018. Biological and phytochemical studies of different leaves extracts of Pteropyrum scoparium. Beni-Suef University Journal of Basic and Applied Sciences, 7(4): 481-486. - Wintola, O.A. and Afolayan, A.J., 2015. The antibacterial, phytochemicals and antioxidants evaluation of the root extracts of Hydnora africana Thunb. used as antidysenteric in Eastern Cape Province, South Africa. BMC Complementary and Alternative Medicine, 15(1): 1-12. - Zhivich, A., 2017. Fighting bacterial resistance: Approaches, challenges, and opportunities in the search for new antibiotics. Part 1. Antibiotics used in clinical practice: Mechanisms of action and the development of bacterial resistance. Microbiology Independent Research journal, 4(1): 31-51. | ||
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