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Forouharmehr, Ali, Banan, A, Mousavi, S. M, Jaydari, A. (1400). Development of a Novel Multi-Epitope Vaccine Candidate against Streptococcus Iniae Infection in Fish: An Immunoinformatics Study. سامانه مدیریت نشریات علمی, 77(1), 45-56. doi: 10.22092/ari.2021.353377.1601
Ali Forouharmehr; A Banan; S. M Mousavi; A Jaydari. "Development of a Novel Multi-Epitope Vaccine Candidate against Streptococcus Iniae Infection in Fish: An Immunoinformatics Study". سامانه مدیریت نشریات علمی, 77, 1, 1400, 45-56. doi: 10.22092/ari.2021.353377.1601
Forouharmehr, Ali, Banan, A, Mousavi, S. M, Jaydari, A. (1400). 'Development of a Novel Multi-Epitope Vaccine Candidate against Streptococcus Iniae Infection in Fish: An Immunoinformatics Study', سامانه مدیریت نشریات علمی, 77(1), pp. 45-56. doi: 10.22092/ari.2021.353377.1601
Forouharmehr, Ali, Banan, A, Mousavi, S. M, Jaydari, A. Development of a Novel Multi-Epitope Vaccine Candidate against Streptococcus Iniae Infection in Fish: An Immunoinformatics Study. سامانه مدیریت نشریات علمی, 1400; 77(1): 45-56. doi: 10.22092/ari.2021.353377.1601

Development of a Novel Multi-Epitope Vaccine Candidate against Streptococcus Iniae Infection in Fish: An Immunoinformatics Study

Archives of Razi Institute
مقاله 8، دوره 77، شماره 1، فروردین و اردیبهشت 2022، صفحه 45-56    اصل مقاله (1.39 M)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2021.353377.1601
نویسندگان
Ali Forouharmehr* 1؛ A Banan2؛ S. M Mousavi3؛ A Jaydari4
1Department of animal science, Faculty of agriculture, Lorestan university, Khorramabad, Iran
2Department of Marine Science, Faculty of Agriculture, Lorestan University, Khorramabad, Iran
3Department of Animal Science, Faculty of Agriculture, Lorestan University, Khorramabad, Iran
4Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran
چکیده
Streptococcus iniae infection is recognized as a disease with substantial economic losses, infecting a wide range of fish species. The limitations of current vaccines and strategies have led to the identification of new methods to control this disease. Multi-epitope vaccines which employ various immunogenic proteins can be promising. The current research project aimed to design an efficient multi-epitope vaccine against Streptococcus iniae infection in fish. To this end, six immunogenic proteins of Streptococcus iniae, including FBA, ENO, Sip11, GAPDH, MtsB, and SCPI proteins, were applied for epitope prediction. The best B cell, T cell, and IFNγ epitopes of the immunogenic proteins, as well as interleukin-8, were used to construct a multi-epitope vaccine. Thereafter, different parameters of the designed vaccine, including physicochemical features, antigenicity, secondary structure, and tertiary structure, were evaluated. Moreover, the interaction of the interleukin-8 domain of the designed vaccine and its receptor was investigated by molecular docking strategy. Finally, nucleotide sequence of the vaccine was adapted to express in Escherichia coli. The results of the present study pointed out that the designed vaccine was a stable vaccine with molecular weight and antigenicity score of 45 kDa and 0.936, respectively. Furthermore, the structure analysis results revealed that the designed vaccine contained 23.49% alpha helix, with 90.5% residues in favored region. Finally, it was demonstrated that the interleukin-8 domain of the designed vaccine could be successfully docked to its receptor with the lowest energy of -1020.9. Based on the obtained results, it seems that the designed vaccine can be an efficient candidate to prevent Streptococcus iniae infection in fish.
کلیدواژه‌ها
Multi-epitope scaffold؛ Streptococcus iniae؛ Immunoinformatics؛ Immunogenic proteins
عنوان مقاله [English]
توسعه یک واکسن چند اپیتوپی جدید علیه عفونت استرپتوکوکوس اینیایی در ماهی: یک مطالعه ایمنوانفورماتیک
چکیده [English]
استرپتوکوکوس اینیایی به عنوان یک عامل عفونی سبب ایجاد خسارات اقتصادی زیادی می‌شود که می‌تواند طیف وسیعی از گونه‌های ماهی را آلوده کند. محدودیت‌ها که در مورد واکسن های رایج و استراتژی‌های موجود وجود دارد منجر به شناسایی روش‌های جدیدی برای کنترل این بیماری می‌شود. در این رابطه، واکسن‌های مبتنی بر چند اپی توپ که بر پایه استفاده از پروتئین‌های مختلف ایمنوژنیک هستند می‌توانند امیدوار کننده باشند. مطالعه حاضر در مورد مهندسی یک واکسن چند اپی-توپی کارآمد علیه عامل استرپتوکوکوس اینیایی است. در این ارتباط، شش پروتئین ایمنوژنیک از استرپتوکوکوس اینیایی که شامل پروتئین هایFBA ، ENO، Sip11،GAPDH ، MtsB و SCPI بود برای پیش بینی اپی توپ استفاده شد. برای ساخت واکسن چند اپی توپی از بهترین اپی‌توپ‌های سلول B ، سلول T و IFNγ پروتئین های مذکور به همراه اینترلوکین-8 استفاده شد. سپس، پارامترهای مختلفی از واکسن طراحی شده از جمله ویژگی‌های فیزیکوشیمیایی، آنتی ژنسیتی، ساختارهای ثانویه و ساختار سوم مورد ارزیابی قرار گرفت. علاوه بر این، تعامل بین دمین اینترلوکین-8 از واکسن مهندسی شده و گیرنده آن توسط فرایند داکینگ مولکولی بررسی شد. سرانجام، توالی نوکلئوتیدی واکسن طراحی شده برای بیان در باکتری اشرشیاکلی سازگار شد. نتایج بدست امده از این پژوهش نشان داد که واکسن طراحی شده با وزن مولکولی 45 کیلو دالتون و اسکور آنتی ژنسیتی 936/0، یک واکسن پایدار است. همچنین، نتایج تجزیه و تحلیل ساختار نشان داد که واکسن طراحی شده شامل 49/23٪ مارپیچ آلفا است که 5/90٪ اسید آمینه‌ها در منطقه مطلوب واقع شده‌اند. در نهایت نیز نتایج داکینگ مولکولی نشان دادکه دمین اینترلوکین-8 از واکسن مهندسی شده می‌تواند با کمترین انرژی( 9/1020- ) به گیرنده خود متصل شود. بر اساس نتایج مستخرج از این مطالعه به نظر می‌رسد که واکسن طراحی شده می-تواند کاندید کارآمدی برای جلوگیری از عفونت استرپتوکوکوس اینیایی باشد
کلیدواژه‌ها [English]
سازه چند اپیتوپی, استرپتوکوکوس اینیایی, ایمنوانفورماتیک, پروتئین‌های ایمنوژنیک
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