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Investigation the effects of different salinities on valuable pigments of halophyte microalgae Cyanothece sp. isolated from back barrier Lipar (Chabahar) in laboratory conditions | ||
| مجله علمی شیلات ایران | ||
| Volume 30, Issue 5, January and February 2022, Pages 121-133 PDF (491.9 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.22092/isfj.2021.125503 | ||
| Authors | ||
| Zahra Amini khooi; Elnaz Erfani far; Ashkan Azhdari; Sima Abir | ||
| Abstract | ||
| One of the main prerequisites for success in the microalgae production industry and the extraction of natural pigments from them is the selection of a suitable microalgae strain for culture. In this study, for the first time, isolation, purification and identification of native strain of Cyanothece sp. Chabahar was taken from Lipar back barrier (salinity up to 400 part per thousand, ppt). In the next step, to find suitable conditions for growing, this microalga was cultured in five different salinity levels (37, 60, 90, 120, 180 ppt) and growth rate, biomass production, chlorophyll a and b pigment content, total carotenoids and phycocyanin were assayed. The growth performance was performed by cell counting and measuring pigments using a spectrophotometer method. The analysis of data showed that increasing salinity from 37 to 90 ppt increased the number of microalgae cells and biomass and the maximum number of cells was 5×106 cells per ml and biomass 1.4 g/L was obtained at a salinity of 90 ppt. The maximum contents of chlorophyll a, chlorophyll b and phycocyanin were obtained (32μg/ml), (17μg/ml) and (2.47 μg/ml) at 90 ppt salinity, respectively. By increasing the salinity from 37 to 120 ppt, the total carotenoids production and beta-carotene also increased significantly, so that the amount of carotenoids reached from 0.8 to 4.67 μg/ml. Beta-carotene also increased from 0.03 to 0.17 mg/ml. Based on this information, optimal growth and accumulation of economic pigments in microalgae Cyanothece sp. can be obtained at salinity 90 to 120 ppt. | ||
| Keywords | ||
| Microalgae; Cyanothece sp; Salinity; Growth; Beta-carotene; Phycocyanin | ||
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